Difference between revisions of "Team:Vilnius-Lithuania/Solution"

 
(9 intermediate revisions by 3 users not shown)
Line 11: Line 11:
 
<div class="wrapper solution-page">
 
<div class="wrapper solution-page">
 
     <div class="container">
 
     <div class="container">
−
         <h1>Our solution</h1>
+
         <h1>Our Solution: the SynORI Framework</h1>
−
         <h5>Lorem ipsum dolor sit amet consectetur</h5>
+
         <h5></h5>
 
         <div class="presentation">
 
         <div class="presentation">
 
             <div class="left">
 
             <div class="left">
Line 21: Line 21:
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 1.</div>
 
                         <div class="step">1. 1.</div>
−
                         <div class="title">Design your ORI</div>
+
                         <div class="title">Plasmid Groups</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>Multi-plasmid framework would not be much without multiple plasmids. The synthetic origin of replication allows to co-maintain different unique plasmid groups in a single cell.</p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 2.</div>
 
                         <div class="step">1. 2.</div>
−
                         <div class="title">Design your ORI</div>
+
                         <div class="title">Copy Number Control</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>With the SynORI system you can adjust your plasmids' copy number in constitutive and inducible manner or virtually any signal pattern.</p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 3.</div>
 
                         <div class="step">1. 3.</div>
−
                         <div class="title">Design your ORI</div>
+
                         <div class="title">Adjusting Copy Number</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>With the SynORI we introduce the world’s first inducible precise copy number plasmids. You may choose any particular plasmid to be low or high copy number.</p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 4.</div>
 
                         <div class="step">1. 4.</div>
−
                         <div class="title">Design your ORI</div>
+
                         <div class="title">Rop </div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>Would you like to globally control your plasmid groups’ copy number, we have optimized the effects of Rop protein. By inducing the expression of Rop you can lower the copy number of all plasmid groups universally. </p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 5.</div>
 
                         <div class="step">1. 5.</div>
−
                         <div class="title">Design your ORI</div>
+
                         <div class="title">Selection System</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>Five different plasmids equals to five different antibiotics for external selection? Not anymore. SynORI contains a five-part self-assembling selection system which uses only one antibiotic resistance gene.</p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 6.</div>
 
                         <div class="step">1. 6.</div>
−
                         <div class="title">Segregation system</div>
+
                         <div class="title">Segregation System</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p>SynORI also contains an active partitioning device, which ensures stable specific plasmid group inheritance and can be used for low copy number plasmids.</p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                     <div class="left-step">
 
                     <div class="left-step">
 
                         <div class="step">1. 7.</div>
 
                         <div class="step">1. 7.</div>
 
                         <div class="title">Your SynORI system overview</div>
 
                         <div class="title">Your SynORI system overview</div>
−
                         <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut
+
                         <p></p>
−
                            labore et dolore magna aliqua. Ut enim ad minim veniam.</p>
+
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
Line 73: Line 66:
 
                         <div class="content visible">
 
                         <div class="content visible">
 
                             <img class="synlogo" src="https://static.igem.org/mediawiki/2017/7/71/T--Vilnius-Lithuania--step-1-1-logo.png" width="228" height="150">
 
                             <img class="synlogo" src="https://static.igem.org/mediawiki/2017/7/71/T--Vilnius-Lithuania--step-1-1-logo.png" width="228" height="150">
−
                             <h3 class="fstep">A framework for multi-plasmid sytems</h3>
+
                             <h3 class="fstep">A framework for multi-plasmid systems</h3>
 
                             <p class="fstep">It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.</p>
 
                             <p class="fstep">It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.</p>
 
                         </div>
 
                         </div>
Line 107: Line 100:
 
                             <div class="s11-center s11-center-ind">
 
                             <div class="s11-center s11-center-ind">
 
                                 <img src="https://static.igem.org/mediawiki/2017/7/7f/T--Vilnius-Lithuania--step-1-2-centerind.png" width="291" height="226">
 
                                 <img src="https://static.igem.org/mediawiki/2017/7/7f/T--Vilnius-Lithuania--step-1-2-centerind.png" width="291" height="226">
−
                                 <div class="cenname">Inducable</div>
+
                                 <div class="cenname">Inducible</div>
 
                             </div>
 
                             </div>
 
                             <div class="s11-center s11-center-osc">
 
                             <div class="s11-center s11-center-osc">
Line 230: Line 223:
 
                         <div class="content">
 
                         <div class="content">
 
                             <h2>Your personal SynORI system is now ready. It is as simple as that.</h2>
 
                             <h2>Your personal SynORI system is now ready. It is as simple as that.</h2>
−
                             <a href="#" class="insol">Explore synori system in detail</a>
+
                             <a href="https://2017.igem.org/Team:Vilnius-Lithuania/Description" class="insol">Explore synori system in detail</a>
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
Line 276: Line 269:
 
                                 <div class="s22-box-radio showsol4" data-rline="4" data-rtype="ind"></div>
 
                                 <div class="s22-box-radio showsol4" data-rline="4" data-rtype="ind"></div>
 
                                 <div class="s22-box-radio showsol5" data-rline="5" data-rtype="ind"></div>
 
                                 <div class="s22-box-radio showsol5" data-rline="5" data-rtype="ind"></div>
−
                                 <div class="abname">Inducable</div>
+
                                 <div class="abname">Inducible</div>
 
                             </div>
 
                             </div>
 
                             <div class="s22-box-radios">
 
                             <div class="s22-box-radios">
Line 297: Line 290:
 
                                         <img src="https://static.igem.org/mediawiki/2017/8/86/T--Vilnius-Lithuania--sol-plasmid1.png" width="37" height="40">
 
                                         <img src="https://static.igem.org/mediawiki/2017/8/86/T--Vilnius-Lithuania--sol-plasmid1.png" width="37" height="40">
 
                                         <div class="s13-line-plasmid-type">Constant</div>
 
                                         <div class="s13-line-plasmid-type">Constant</div>
−
                                         <div class="s13-line-plasmid-type">Inducable</div>
+
                                         <div class="s13-line-plasmid-type">Inducible</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                     </div>
 
                                     </div>
Line 320: Line 313:
 
                                         <img src="https://static.igem.org/mediawiki/2017/4/47/T--Vilnius-Lithuania--sol-plasmid2.png" width="37" height="39">
 
                                         <img src="https://static.igem.org/mediawiki/2017/4/47/T--Vilnius-Lithuania--sol-plasmid2.png" width="37" height="39">
 
                                         <div class="s13-line-plasmid-type">Constant</div>
 
                                         <div class="s13-line-plasmid-type">Constant</div>
−
                                         <div class="s13-line-plasmid-type">Inducable</div>
+
                                         <div class="s13-line-plasmid-type">Inducible</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                     </div>
 
                                     </div>
Line 343: Line 336:
 
                                         <img src="https://static.igem.org/mediawiki/2017/c/c1/T--Vilnius-Lithuania--sol-plasmid3.png" width="37" height="39">
 
                                         <img src="https://static.igem.org/mediawiki/2017/c/c1/T--Vilnius-Lithuania--sol-plasmid3.png" width="37" height="39">
 
                                         <div class="s13-line-plasmid-type">Constant</div>
 
                                         <div class="s13-line-plasmid-type">Constant</div>
−
                                         <div class="s13-line-plasmid-type">Inducable</div>
+
                                         <div class="s13-line-plasmid-type">Inducible</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                     </div>
 
                                     </div>
Line 366: Line 359:
 
                                         <img src="https://static.igem.org/mediawiki/2017/c/ce/T--Vilnius-Lithuania--sol-plasmid4.png" width="37" height="39">
 
                                         <img src="https://static.igem.org/mediawiki/2017/c/ce/T--Vilnius-Lithuania--sol-plasmid4.png" width="37" height="39">
 
                                         <div class="s13-line-plasmid-type">Constant</div>
 
                                         <div class="s13-line-plasmid-type">Constant</div>
−
                                         <div class="s13-line-plasmid-type">Inducable</div>
+
                                         <div class="s13-line-plasmid-type">Inducible</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                     </div>
 
                                     </div>
Line 389: Line 382:
 
                                         <img src="https://static.igem.org/mediawiki/2017/d/d0/T--Vilnius-Lithuania--sol-plasmid5.png" width="37" height="39">
 
                                         <img src="https://static.igem.org/mediawiki/2017/d/d0/T--Vilnius-Lithuania--sol-plasmid5.png" width="37" height="39">
 
                                         <div class="s13-line-plasmid-type">Constant</div>
 
                                         <div class="s13-line-plasmid-type">Constant</div>
−
                                         <div class="s13-line-plasmid-type">Inducable</div>
+
                                         <div class="s13-line-plasmid-type">Inducible</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                         <div class="s13-line-plasmid-type">Oscillating</div>
 
                                     </div>
 
                                     </div>

Latest revision as of 02:08, 16 November 2017

use keyboard, swipe or scroll

Our Solution: the SynORI Framework

1. 1.
Plasmid Groups

Multi-plasmid framework would not be much without multiple plasmids. The synthetic origin of replication allows to co-maintain different unique plasmid groups in a single cell.

1. 2.
Copy Number Control

With the SynORI system you can adjust your plasmids' copy number in constitutive and inducible manner or virtually any signal pattern.

1. 3.
Adjusting Copy Number

With the SynORI we introduce the world’s first inducible precise copy number plasmids. You may choose any particular plasmid to be low or high copy number.

1. 4.
Rop

Would you like to globally control your plasmid groups’ copy number, we have optimized the effects of Rop protein. By inducing the expression of Rop you can lower the copy number of all plasmid groups universally.

1. 5.
Selection System

Five different plasmids equals to five different antibiotics for external selection? Not anymore. SynORI contains a five-part self-assembling selection system which uses only one antibiotic resistance gene.

1. 6.
Segregation System

SynORI also contains an active partitioning device, which ensures stable specific plasmid group inheritance and can be used for low copy number plasmids.

1. 7.
Your SynORI system overview

A framework for multi-plasmid systems

It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.

Constant
Inducible
Oscillating

Your personal SynORI system is now ready. It is as simple as that.

Explore synori system in detail
0
Select at least 2!

Please select plasmid number that will appear in a cell.

Constant
Inducible
Oscillating
Try selecting all types!
Select type of copy number control
Constant
Inducible
Oscillating
Oscillation period
Constant
Inducible
Oscillating
Oscillation period
Constant
Inducible
Oscillating
Oscillation period
Constant
Inducible
Oscillating
Oscillation period
Constant
Inducible
Oscillating
Oscillation period
Preview of Rop induction

Choose level of ROP induction

Off
Medium
High

Do you want to add ROP protein to your SynORI system?

Yes
No
Transcription factor
Riboswitch trigger
Riboswitch
248
+
248
+
248
+
38-415
+
248
+
Plasmid group
Type of copy number control
Copy number
SynORI selection system
Segregation system
This might be helpful:

If your copy number is lower than 30, we recommend checking the box.

248
+
248
+
248
+
38-415
+
248
+
Plasmid group
Type of copy number control
Copy number
SynORI selection system
Segregation system
ROP PROTEIN is not included
Level of ROP induction: Off
SYNORI