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<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 1.</div> | <div class="step">1. 1.</div> | ||
− | <div class="title"> | + | <div class="title">Plasmid Groups</div> |
− | <p> | + | <p>Multi-plasmid framework would not be much without multiple plasmids. The synthetic origin of replication allows to co-maintain different unique plasmid groups in a single cell.</p> |
− | + | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 2.</div> | <div class="step">1. 2.</div> | ||
− | <div class="title"> | + | <div class="title">Copy Number Control</div> |
− | <p> | + | <p>With the SynORI system you can adjust your plasmids' copy number in constitutive and inducible manner or virtually any signal pattern.</p> |
− | + | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 3.</div> | <div class="step">1. 3.</div> | ||
− | <div class="title"> | + | <div class="title">Adjusting Copy Number</div> |
− | <p> | + | <p>With the SynORI we introduce the world’s first inducible precise copy number plasmids. You may choose any particular plasmid to be low or high copy number.</p> |
− | + | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 4.</div> | <div class="step">1. 4.</div> | ||
− | <div class="title"> | + | <div class="title">Rop </div> |
<p>Would you like to globally control your plasmid groups’ copy number, we have optimized the effects of Rop protein. By inducing the expression of Rop you can lower the copy number of all plasmid groups universally. </p> | <p>Would you like to globally control your plasmid groups’ copy number, we have optimized the effects of Rop protein. By inducing the expression of Rop you can lower the copy number of all plasmid groups universally. </p> | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 5.</div> | <div class="step">1. 5.</div> | ||
− | <div class="title"> | + | <div class="title">Selection System</div> |
− | <p> | + | <p>Five different plasmids equals to five different antibiotics for external selection? Not anymore. SynORI contains a five-part self-assembling selection system which uses only one antibiotic resistance gene.</p> |
− | + | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 6.</div> | <div class="step">1. 6.</div> | ||
− | <div class="title">Segregation | + | <div class="title">Segregation System</div> |
− | <p> | + | <p>SynORI also contains an active partitioning device, which ensures stable specific plasmid group inheritance and can be used for low copy number plasmids.</p> |
− | + | ||
</div> | </div> | ||
<div class="left-step"> | <div class="left-step"> | ||
<div class="step">1. 7.</div> | <div class="step">1. 7.</div> | ||
<div class="title">Your SynORI system overview</div> | <div class="title">Your SynORI system overview</div> | ||
− | <p> | + | <p></p> |
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="content visible"> | <div class="content visible"> | ||
<img class="synlogo" src="https://static.igem.org/mediawiki/2017/7/71/T--Vilnius-Lithuania--step-1-1-logo.png" width="228" height="150"> | <img class="synlogo" src="https://static.igem.org/mediawiki/2017/7/71/T--Vilnius-Lithuania--step-1-1-logo.png" width="228" height="150"> | ||
− | <h3 class="fstep">A framework for multi-plasmid | + | <h3 class="fstep">A framework for multi-plasmid systems</h3> |
<p class="fstep">It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.</p> | <p class="fstep">It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.</p> | ||
</div> | </div> | ||
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<div class="s11-center s11-center-ind"> | <div class="s11-center s11-center-ind"> | ||
<img src="https://static.igem.org/mediawiki/2017/7/7f/T--Vilnius-Lithuania--step-1-2-centerind.png" width="291" height="226"> | <img src="https://static.igem.org/mediawiki/2017/7/7f/T--Vilnius-Lithuania--step-1-2-centerind.png" width="291" height="226"> | ||
− | <div class="cenname"> | + | <div class="cenname">Inducible</div> |
</div> | </div> | ||
<div class="s11-center s11-center-osc"> | <div class="s11-center s11-center-osc"> | ||
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<div class="content"> | <div class="content"> | ||
<h2>Your personal SynORI system is now ready. It is as simple as that.</h2> | <h2>Your personal SynORI system is now ready. It is as simple as that.</h2> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:Vilnius-Lithuania/Description" class="insol">Explore synori system in detail</a> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="s22-box-radio showsol4" data-rline="4" data-rtype="ind"></div> | <div class="s22-box-radio showsol4" data-rline="4" data-rtype="ind"></div> | ||
<div class="s22-box-radio showsol5" data-rline="5" data-rtype="ind"></div> | <div class="s22-box-radio showsol5" data-rline="5" data-rtype="ind"></div> | ||
− | <div class="abname"> | + | <div class="abname">Inducible</div> |
</div> | </div> | ||
<div class="s22-box-radios"> | <div class="s22-box-radios"> | ||
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<img src="https://static.igem.org/mediawiki/2017/8/86/T--Vilnius-Lithuania--sol-plasmid1.png" width="37" height="40"> | <img src="https://static.igem.org/mediawiki/2017/8/86/T--Vilnius-Lithuania--sol-plasmid1.png" width="37" height="40"> | ||
<div class="s13-line-plasmid-type">Constant</div> | <div class="s13-line-plasmid-type">Constant</div> | ||
− | <div class="s13-line-plasmid-type"> | + | <div class="s13-line-plasmid-type">Inducible</div> |
<div class="s13-line-plasmid-type">Oscillating</div> | <div class="s13-line-plasmid-type">Oscillating</div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2017/4/47/T--Vilnius-Lithuania--sol-plasmid2.png" width="37" height="39"> | <img src="https://static.igem.org/mediawiki/2017/4/47/T--Vilnius-Lithuania--sol-plasmid2.png" width="37" height="39"> | ||
<div class="s13-line-plasmid-type">Constant</div> | <div class="s13-line-plasmid-type">Constant</div> | ||
− | <div class="s13-line-plasmid-type"> | + | <div class="s13-line-plasmid-type">Inducible</div> |
<div class="s13-line-plasmid-type">Oscillating</div> | <div class="s13-line-plasmid-type">Oscillating</div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2017/c/c1/T--Vilnius-Lithuania--sol-plasmid3.png" width="37" height="39"> | <img src="https://static.igem.org/mediawiki/2017/c/c1/T--Vilnius-Lithuania--sol-plasmid3.png" width="37" height="39"> | ||
<div class="s13-line-plasmid-type">Constant</div> | <div class="s13-line-plasmid-type">Constant</div> | ||
− | <div class="s13-line-plasmid-type"> | + | <div class="s13-line-plasmid-type">Inducible</div> |
<div class="s13-line-plasmid-type">Oscillating</div> | <div class="s13-line-plasmid-type">Oscillating</div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2017/c/ce/T--Vilnius-Lithuania--sol-plasmid4.png" width="37" height="39"> | <img src="https://static.igem.org/mediawiki/2017/c/ce/T--Vilnius-Lithuania--sol-plasmid4.png" width="37" height="39"> | ||
<div class="s13-line-plasmid-type">Constant</div> | <div class="s13-line-plasmid-type">Constant</div> | ||
− | <div class="s13-line-plasmid-type"> | + | <div class="s13-line-plasmid-type">Inducible</div> |
<div class="s13-line-plasmid-type">Oscillating</div> | <div class="s13-line-plasmid-type">Oscillating</div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2017/d/d0/T--Vilnius-Lithuania--sol-plasmid5.png" width="37" height="39"> | <img src="https://static.igem.org/mediawiki/2017/d/d0/T--Vilnius-Lithuania--sol-plasmid5.png" width="37" height="39"> | ||
<div class="s13-line-plasmid-type">Constant</div> | <div class="s13-line-plasmid-type">Constant</div> | ||
− | <div class="s13-line-plasmid-type"> | + | <div class="s13-line-plasmid-type">Inducible</div> |
<div class="s13-line-plasmid-type">Oscillating</div> | <div class="s13-line-plasmid-type">Oscillating</div> | ||
</div> | </div> |
Latest revision as of 02:08, 16 November 2017
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Our Solution: the SynORI Framework
Multi-plasmid framework would not be much without multiple plasmids. The synthetic origin of replication allows to co-maintain different unique plasmid groups in a single cell.
With the SynORI system you can adjust your plasmids' copy number in constitutive and inducible manner or virtually any signal pattern.
With the SynORI we introduce the world’s first inducible precise copy number plasmids. You may choose any particular plasmid to be low or high copy number.
Would you like to globally control your plasmid groups’ copy number, we have optimized the effects of Rop protein. By inducing the expression of Rop you can lower the copy number of all plasmid groups universally.
Five different plasmids equals to five different antibiotics for external selection? Not anymore. SynORI contains a five-part self-assembling selection system which uses only one antibiotic resistance gene.
SynORI also contains an active partitioning device, which ensures stable specific plasmid group inheritance and can be used for low copy number plasmids.
A framework for multi-plasmid systems
It is time to make the work with plasmid systems more precise, convenient and extend its possibilities.
Your personal SynORI system is now ready. It is as simple as that.
Explore synori system in detailPlease select plasmid number that will appear in a cell.
Preview of Rop induction
Choose level of ROP induction
Do you want to add ROP protein to your SynORI system?
This might be helpful:
If your copy number is lower than 30, we recommend checking the box.