Difference between revisions of "Team:Newcastle/Attributions"

 
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Team Leader: Jessica Birt <br />
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Second Team Leader: Declan Kohl
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Experimental: Valeria Verrone (Head)
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IPTG Sensor: Declan Kohl, Jessica Birt
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Arsenic Biosensor: Valeria Verrone (characterisation), Declan Kohl (design)
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Sarcosine Oxidase: Sophie Badger (characterisation), Jessica Birt (design)
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FimE Switch: Marcia Pryce (characterisation), Bradley Brown (design)
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Cell Free Protein Synthesis Systems: Bradley Brown
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deGFP: Sophie Badger, Bradley Brown, Lais Takiguchi
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Chromoproteins: Sophie Badger, Lais Takiguchi
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Processing Variants: Jessica Birt, Marcia Pryce, Lais Takiguchi
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Synthetic Promoter Library: Lais Takiguchi
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Fluorescene Normalisation: Michaela Chapman, Ansh Vyas
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Assembly Methods: Anna Walsh, Michaela Chapman, Evie Whittaker
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Microfluidics Software: Jack Cooper
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Robotics: Jessica Birt, Declan Kohl
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Interlab Study: Michaela Chapman (Head), Declan Kohl, Zoe Wilson, Marcia Pryce, Ansh Vyas
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Human Practices: Declan Kohl (Head), Zoe Wilson, Jessica Birt
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Integrated HP: Declan Kohl, Jessica Birt
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Outreach and Education: Declan Kohl, Zoe Wilson, Sophie Badger, Marcia Pryce
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Scientific Communication Analysis: Zoe Wilson<br />
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Modelling: Jessica Birt (Head), Bradley Brown
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Simbiotics: Jessica Birt
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Design of Experiments: Bradley Brown
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Wiki: Declan Kohl, Bradley Brown, Jack Cooper, Marcia Pryce
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Poster Development: All Team Members
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Presentation Development: All Team Members
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Presenters: Declan Kohl
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      <h1 class="text-center" style="font-family: Rubik; margin-bottom: 2%">Attributions</h1>
  
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We aim to produce a toolbox that can be used to construct biosensors. This toolbox will contain devices which can be used to construct biosensors. As the toolbox will contain various devices, as well as chassis, several properties and functionalities for biosensors are possible. To assist with design and development the toolbox will contain in silico tools.<br />
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We also aim to develop a biosensor development technique which allows easy and rapid development and testing of biosensor variants. Each device in our toolbox can be expressed in a separate cell and co-cultured with other cells expressing different devices to produce a functional biosensor. For example, three cell strains expressing an inducible promoter (detector device), a signal amplification device (processing device), and a fluorescent protein (reporter device) can be co-cultured together to make a biosensor. Devices in each cell will communicate through the use of 'connectors', which take the form of quorum sensing molecules.<br />
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To surpass beyond the lab we want to contribute to the biological community. We aim to highlight the need for our toolbox, by conducting an investigation into the progression of biosensors from research to distribution, as well as the availability of and demand for biosensors.  How often biosensors successfully progress from design to distribution, and identification of and issues encountered at each stage of this progression will be investigated, and the results implemented into the toolbox, aiming to help overcome these issues.
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      <p style="clear: both; margin-bottom: 2%">This year our team consisted of 8 molecular biologists, a life scientist, a computer scientist, a forensic scientist, a chemical engineer and an English student. We bring different branches of expertise together and are a true example of an interdisciplinary team. We decided on our project idea during April, after months of intense and independent discussions. Unless otherwise stated, all the experiments, constructs, code, wiki and results documented on our Wiki were performed, written and collected by the Sensynova team.</p>
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      <p style="float: left; margin-bottom: 2%"><b>Team Leader</b>: Jessica Birt</p>
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      <p style="float: right; margin-bottom: 2%"><b>Vice Team Leader</b>: Declan Kohl</p>
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        <a class="nav-item nav-link active" id="nav-experimental-tab" data-toggle="tab" href="#nav-experimental" role="tab" aria-controls="nav-experimental" aria-selected="true">Team Members</a>
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        <a class="nav-item nav-link" id="nav-hp-tab" data-toggle="tab" href="#nav-hp" role="tab" aria-controls="nav-hp" aria-selected="false">Supervisors and Advisers</a>
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        <a class="nav-item nav-link" id="nav-model-tab" data-toggle="tab" href="#nav-model" role="tab" aria-controls="nav-model" aria-selected="false">Additional Acknowledgements</a>
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        <b>Experimental Lead</b>: Valeria Verrone</br>
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        <b>Sensynova Framework Proof of Concept</b>: Jessica Birt (design), Valeria Verrone, Declan Kohl (characterisation) </br>
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        <b>Arsenic Biosensor</b>: Valeria Verrone (characterisation), Declan Kohl (design) </br>
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        <b>Sarcosine Oxidase</b>: Sophie Badger (characterisation), Jessica Birt (design) </br>
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        <b>FimE Switch</b>: Marcia Pryce (characterisation), Bradley Brown (design) </br>
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      <b> Cell Free Protein Synthesis Systems</b>: Bradley Brown </br>
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        <b>deGFP</b>: Bradley Brown (design and characterisation), Sophie Badger (implementation) </br>
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        <b>Chromoproteins</b>: Lais Takiguchi (design, implementation, characterisation), Valeria Verrone (characterisation within framework) </br>
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        <b>Processing Variants</b>: Jessica Birt (design), Lais Takiguchi (characterisation) </br>
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        <b>Synthetic Promoter Library</b>: Lais Takiguchi </br>
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        <b>Fluorescene Normalisation</b>: Michaela Chapman, Ansh Vyas </br>
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        <b>Assembly Methods</b>: Anna Walsh, Michaela Chapman, Evie Whittaker </br>
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      <b> Microfluidics Software</b>: Jack Cooper </br>
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        <b>Interlab Study</b>: Michaela Chapman (Head), Declan Kohl, Zoe Wilson, Marcia Pryce, Ansh Vyas</br>
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        </p>
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        <b>Human Practices Lead</b>: Declan Kohl</br>
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        <b>Integrated HP</b>: Declan Kohl, Jessica Birt </br>
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        <b>Outreach and Education</b>: Zoe Wilson (lead), Declan Kohl, Sophie Badger, Marcia Pryce </br>
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        <b>Scientific Communication Analysis</b>: Zoe Wilson, Declan Kohl</br>
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        </p>
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        <p class="text-left" style="font-family: Rubik; margin-top: 2%">
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        <b>Modelling Lead</b>: Jessica Birt</br>
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        <b>Simbiotics</b>: Jessica Birt, Bradley Brown </br>
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        <b>Design of Experiments for CFPS</b>: Bradley Brown </br>
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        <b>Microfluidics Software</b>: Jack Cooper</br>
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        </p>
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        <b>Wiki Coding and Design</b>: Jack Cooper, Bradley Brown</br>
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        <b>Poster Development</b>: All Team Members</br>
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        <b>Presentation</b>: Declan Kohl, Sophie Badger and Zoe Wilson</br>
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        All supervisors and advisers gave advice to the team over a range of areas, including the initial project idea and help in the laboratory.</br></br>
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        <b>Anil Wipat</b>: PI - general advice</br>
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        <b>Thomas Howard</b>: Second PI - general advice</br>
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        <b>Jon Marles-Wright</b>: Instructor - general advice</br>
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        <b>Dana Ofiteru</b>: Instructor - general advice</br>
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        <b>Angel Goñi-Moreno</b>: Instructor - modelling advice</br>
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        <b>Wendy Smith</b>: Adviser - general and laboratory advice</br>
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        <b>Michael White</b>: Adviser - laboratory advice</br>
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        <b>Alice Banks</b>: Adviser - laboratory advice</br>
  
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        <b>Jonny Naylor</b>: Help with the modelling aspect of the project, specifically with his Simbiotics software (which he customised to our needs!)</br>
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        <b>James McLaughlin</b>: Assisted us with the technical aspects of our wiki.</br>
  
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        <b>Exeter iGEM 2017</b>: Analysed our deGFP construct for us using their fluorescence Activated Cell Sorting machine.</br>
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        <b>Evry Paris-Saclay 2017</b>: collaborated with us to test their novel synthetic promoter regulated by psicose in our Sensynova framework.</br>
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<b>Dr Chris French, Dr Martin Peacock, Dr Karen Polizzi</b> and <b>Dr Oliver Purcell</b> for their invaluable insight into biosensor development and their help in influencing our project.</br>
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<b>Ivy Wellman from DEFRA</b>: For her insight into the legislative procedure deliberate release of GMOs into the environment.</br>
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<b>Jasmine Bird</b>: Took time to help proof-read the wiki and suggest improvements.<br />
  
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Attributions</h1>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
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This is a bronze medal requirement. Please see the <a href="https://2017.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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<h5> Why is this page needed? </h5>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<li>Project support and advice</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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<h5>Inspiration</h5>
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<p>Take a look at what other teams have done:</p>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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Latest revision as of 21:24, 16 November 2017

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Attributions












This year our team consisted of 8 molecular biologists, a life scientist, a computer scientist, a forensic scientist, a chemical engineer and an English student. We bring different branches of expertise together and are a true example of an interdisciplinary team. We decided on our project idea during April, after months of intense and independent discussions. Unless otherwise stated, all the experiments, constructs, code, wiki and results documented on our Wiki were performed, written and collected by the Sensynova team.


Team Leader: Jessica Birt

Vice Team Leader: Declan Kohl