Difference between revisions of "Team:Toronto/InterLab"

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<h1>Interlab</h1>
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<h1>Dry Lab</h1>
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<h2 class="text-yellow">content-yellow</h2>
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<h2 class="text-red">Description</h2>
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<p>iGEM Toronto was excited to participate in the 2017 Interlab Study to help establish a GFP fluorescence measurement protocol and characterize important promoters and RBS devices. In science and engineering, standardization is an important key to generating reliable data and comparing results from different labs. By comparing our data with labs from around the world, iGEM will be able to establish common standards of measurement for GFP fluoresecence with different plate readers.</p>
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<div class="image"><img src="http://www.excel-easy.com/data-analysis/images/charts/column-chart.png" alt="data"></div>
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<figcaption>Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population</figcaption>
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<h2 class="text-red">Plasmids</h2>
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<p>iGEM HQ provided 8 Interlab parts in total, all in the  pSB1C3 backbone. They included a positive control, a negative control, and 6 test devices which contained various combinations of three promoters and two RBS devices. The positive control contained BBa_J23151, a 35 bp constitutive promoter, and BBa_B0032, a 13 bp weak RBS, while the negative control consisted only of BBa_R0040, a 54 bp TetR repressible promoter.</p>
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<figcaption>Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population</figcaption>
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<h3>All the parts:</h3>
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<li>BBa_E0040: a 720 bp GFP gene from the Aequeora victoria jellyfish </li>
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<li>Fluorescence is expected within 8 minutes </li>
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<div class="image"><img src="https://zippy.gfycat.com/MedicalAdventurousCanine.gif" alt="data" width="200px"></div>
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<li>Excitation max: 501 nm </li>
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<li>Emission max: 511 nm </li>
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<li>BBa_B0010: an 80 bp transcriptional terminator from E. coli rrnB with 64 bp stem loop </li>
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<figcaption>Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population</figcaption>
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<li>BBa_B0012: a 41 bp transcriptional terminator from coliphageT7 for E. coli RNA Polymerase </li>
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<li>Not a good terminator </li>
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<li>promoter in reverse direction </li>
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<h3>The test devices were all 918 bp plasmids which constitutively express GFP. All consist of:</h3>
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<li>A 35 bp constitutive promoter in the BBa_J23100-J23119 family </li>
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<li>1 and 4 have BBa_J23101 </li>
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<li>2 and 5 have BBa_J23106 - medium strength; more characterization info from Sheffield 2016 </li>
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<li>3 and 6 have BBa_J23117 </li>
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<li>A 12 bp RBS, </li>
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<li>1-3 have BBa_B0034, a 12 bp RBS </li>
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<li>4-6 have BBa_J364100, an 84 bp bicistronic design element (BCD2) </li>
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<li>BBa_E0040 </li>
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<li>BBa_B0012</li>
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<th>Part</th>
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<th>Time</th>
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<th>Part number</th>
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<th>Quantity</th>
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<th>Length (bp)</th>
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<th>iunno</th>
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<th>Promoter</th>
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<th>RBS</th>
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<th>Reporter Protein</th>
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<td>Positive control</td>
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<td>Hello</td>
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<td>BBa_I20270</td>
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<td>9adsf asd</td>
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<td>919</td>
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<td>2 ads fads fdsa s </td>
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<td>BBa_J23151</td>
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<td>BBa_B0032</td>
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<td>BBa_E0040 (GFP)</td>
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<td>1</td>
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<td>BBa_B0010 and BBa_B0012</td>
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<td>2 ads fads fdsa s </td>
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<td>2 ads fads fdsa s </td>
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<td>Negative control</td>
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<td>BBa_R0040</td>
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<td>1</td>
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<td>54</td>
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<td>2</td>
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<td>N/A</td>
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<td>2 ads fads fdsa s </td>
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<td>N/A</td>
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<td>N/A</td>
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<td>1</td>
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<td>2</td>
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<td>2 ads fads fdsa s </td>
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<td>Test device 1</td>
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<td>BBa_J364000</td>
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<td>918</td>
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<td>Hello</td>
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<td>BBa_J23101</td>
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<td>911 asd afd </td>
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<td>BBa_B0034</td>
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<td>2 ads fads fdsa s </td>
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<td>BBa_E0040 (GFP)</td>
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<td>BBa_B0010 and BBa_B0012</td>
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<td>1</td>
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<td>Test device 2</td>
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<td>2 ads fads fdsa s </td>
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<td>BBa_J364001</td>
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<td>918</td>
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<td>BBa_J23106</td>
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<td>Test device 3</td>
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<td>BBa_J364002</td>
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<td>BBa_J23117</td>
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<td>Test device 4</td>
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<td>BBa_J364003</td>
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<td>918</td>
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<td>BBa_J23101</td>
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<td>BBa_J364100</td>
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<td>Test device 5</td>
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<td>BBa_J364004</td>
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<td>918</td>
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<td>Test device 6</td>
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<td>BBa_J364005</td>
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<figcaption>Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population</figcaption>
 
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<h2 class="text-red">Results</h2>
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Revision as of 20:27, 17 November 2017

Dry Lab

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content-yellow

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data
Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population
data
Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population

Lorem is haha ipsum dolor sit amet consectetur adipisicing elit. Inventore maiores quibusdam, adipisci ipsum quisquam, aspernatur aperiam optio odit deleniti eaque illum nobis, non neque reprehenderit consequatur ipsam ullam perferendis magni.

data
data
Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population

Lorem is haha ipsum dolor sit amet consectetur adipisicing elit. Inventore maiores quibusdam, adipisci ipsum quisquam, aspernatur aperiam optio odit deleniti eaque illum nobis, non neque reprehenderit consequatur ipsam ullam perferendis magni.

Lorem is haha ipsum dolor sit amet consectetur adipisicing elit. Inventore maiores quibusdam, adipisci ipsum quisquam, aspernatur aperiam optio odit deleniti eaque illum nobis, non neque reprehenderit consequatur ipsam ullam perferendis magni.

Time Quantity iunno
Hello 9adsf asd 2 ads fads fdsa s
1 2 ads fads fdsa s 2 ads fads fdsa s
1 2 2 ads fads fdsa s
1 2 2 ads fads fdsa s
Hello 911 asd afd 2 ads fads fdsa s
1 2 2 ads fads fdsa s
Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population

Lorem is haha ipsum dolor sit amet consectetur adipisicing elit. Inventore maiores quibusdam, adipisci ipsum quisquam, aspernatur aperiam optio odit deleniti eaque illum nobis, non neque reprehenderit consequatur ipsam ullam perferendis magni.

References

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General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto