EllaKestrel (Talk | contribs) |
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− | Working hard, we managed to successfully clone the Esterase gene into E.coli. Both colony PCR and sequencing were positive for the CE 5 esterase clone. | + | Working hard, we managed to successfully clone the Esterase gene into E.coli. Both colony PCR and sequencing were positive for the CE 5 esterase clone. However, Lipase was not successfully cloned and the sequences came up negative and truncated.</article> |
+ | <article> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ee/BaltimoreBiocrewFigure1.png" style="width:960px; height: 540px;"><!--original size: 1,920 × 1,080 pixels--> | ||
+ | </article> | ||
<article> | <article> | ||
Due to the negative results with the lipase, we hypothesized that the lipase enzyme was toxic to the strain of E. coli we were using, causing it to reject the DNA. | Due to the negative results with the lipase, we hypothesized that the lipase enzyme was toxic to the strain of E. coli we were using, causing it to reject the DNA. | ||
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</a> | </a> | ||
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− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/6/6c/T--Baltimore_Bio-Crew--fabian_kolker_small_icon.png" alt="Fabian Kolker Foundation" style="width:100px; height:100px;"> |
</a> | </a> | ||
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--Baltimore_Biocrew--VWR_Foundation_LOGO.jpeg" alt="VWR Charitable Foundation" style="width:100px; height:100px;"> | <img src="https://static.igem.org/mediawiki/2016/1/1a/T--Baltimore_Biocrew--VWR_Foundation_LOGO.jpeg" alt="VWR Charitable Foundation" style="width:100px; height:100px;"> | ||
</a> | </a> | ||
− | <a href="http:// | + | <a href="http://www.marylandrecyclingnetwork.org/"> |
<img src="https://media.licdn.com/mpr/mpr/shrink_200_200/AAEAAQAAAAAAAAI8AAAAJDY0ZDg0ZjlkLWVlMTItNGI1Mi1iNWEwLWYzMDVlYWMwMTZhZg.png" alt="Maryland Recycling Network" style="width:100px; height:100px;"> | <img src="https://media.licdn.com/mpr/mpr/shrink_200_200/AAEAAQAAAAAAAAI8AAAAJDY0ZDg0ZjlkLWVlMTItNGI1Mi1iNWEwLWYzMDVlYWMwMTZhZg.png" alt="Maryland Recycling Network" style="width:100px; height:100px;"> | ||
+ | </a> | ||
+ | |||
+ | <a href="https://www.rwdfoundation.org/"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/6/65/T--Baltimore_BioCrew--DeutschFoundation.png" alt="The Robert W. Deutsch Foundation" style="width:100px; height:100px;"> | ||
</a> | </a> | ||
Latest revision as of 19:40, 19 November 2017
![BUGSS Logo](https://static.igem.org/mediawiki/2017/e/e2/Baltimore_biocre_BugssLOGO.png)
BALTIMORE BIO-CREW
Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor
Results
![](https://static.igem.org/mediawiki/2017/e/ee/BaltimoreBiocrewFigure1.png)
![](https://static.igem.org/mediawiki/2017/5/5e/T--Baltimore_Bio-Crew--results_fig2.jpg)
![](https://static.igem.org/mediawiki/2017/e/ec/T--Baltimore_Bio-Crew--results_fig3.jpg)
![](https://static.igem.org/mediawiki/2017/3/38/T--Baltimore_Bio-Crew--results_fig4.jpg)