Difference between revisions of "Team:Baltimore Bio-Crew/Composite Part"

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<html>
 
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<!-- This is the tab title styling-->
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      <head>
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        <title>Baltimore Bio-Crew</title>
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        <link rel="icon" type="image/png" sizes="16x16" href="http://icons.iconarchive.com/icons/glyphish/glyphish/32/91-beaker-2-icon.png">
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        <meta charset= "utf-8">
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        <link rel="stylesheet" href="style.css" type="text/css"
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        <meta http-equiv="x-ua-compatible" content="ie=edge">
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        <link href="https://fonts.googleapis.com/css?family=Saira|Bubbler+One|Abel|Source+Sans+Pro|Share+Tech+Mono|Kalam" rel="stylesheet">
  
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<style>
  
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<div class="column full_size judges-will-not-evaluate">
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article{
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<h3>★ ALERT! </h3>
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width: 50%;
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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}
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.BbP img {
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box-shadow: 0 10px 20px 0 rgba(71, 232, 247, 1);
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margin-bottom:30px;
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margin-left: 7%;
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display: inline;
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font-style: italic;
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font-family: 'Abel', san-serif;
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color: black;
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<h1>Composite Parts</h1>
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margin-left:30%;
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  font-family: 'Abel', san-serif;
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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</p>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
  
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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font-size: 16px;
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<br>
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<div class="highlight">
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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      </head>
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      <body>
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<section>
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<header>
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        <div class="Intro">
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          <h1>BALTIMORE BIO-CREW</h1>
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          <h4>Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor</h4>
 
</div>
 
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</section>
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<section id="description" class= "BbP">
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<header>
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        <h3>Composite Parts</h3>
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</header>
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    <article>
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We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. The chlorogenate esterase construct (BBa_K2436001) was cloned successfully and submitted to the registry. The lipase construct was not cloned successfully. Our constructs were designed as follows.
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</article>     
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<img src="https://static.igem.org/mediawiki/2017/a/a5/T--Baltimore_Bio-Crew--small_construct_diagrams.png" style="width: 1000px; height:562px; margin-left:auto;margin-right:auto; display: block;" >
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<article>
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<strong>Lactose Regulated Promoter (BBa_R0011):</strong> a DNA sequence that starts the transcription process in the presence of lactose or IPTG. Thus, the promoter is only active in certain times to regulate the amount of proteins the bacteria is able to produce at a time. The promoter causes the gene to activate and begin the process of forming mRNA and eventually proteins.
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</article>
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<article>
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<strong>Ribosome Binding Site (BBa_B0030):</strong> an RNA sequence found in mRNA to which ribosomes can bind and initiate translation. It is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation.
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</article>
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<article>
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<strong>PelB Secretion Tag (BBa_K2010002):</strong> allows for bacteria to secrete the enzymes (lipase and esterase) into the periplasm. Once the enzymes are secreted to the periplasm, they are able to exit the cell on their own.
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</article>
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<article>
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<strong>Protein Coding Sequence (BBa_K2187001 for esterase or BBa_K2187000 for lipase):</strong> the DNA sequence is the lipase and esterase gene sequences. The lipase and esterase are both needed to successfully produce the plastic degrading enzymes because both work together to break down and eliminate the plastic particles. One construct contains the chlorogenate esterase coding sequence, and the other construct has the lipase coding sequence.
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</article>
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<article>
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<strong>His Tag:</strong> allows us to purify the protein on a nickel column once it’s secreted outside of the bacteria
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</article>
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<article>
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<strong>Terminator (BBa_K864600):</strong> a sequence that occurs at the end of the gene and causes transcription to stop
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</article>
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</section>
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<section id="Footer" class="footerSection">
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<hr>
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<h2>
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Sponsors
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</h2>
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<h4>
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The Baltimore Bio-Crew thanks our sponsors for their generous support of our team that made our project and travel to the Jamboree possible. Thank you!
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</h4>
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<footer>
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<a href="http://www.bd.com/en-us">
 +
  <img src="https://upload.wikimedia.org/wikipedia/en/f/f8/Update_Color_BD_PNG_Logo.png" alt="BD Medical Technology, Advancing the World of Health - BD" style="width:100px; height:100px;">
 +
</a>
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<a href="http://familyleague.org/">
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  <img src="http://baltimoreattendance.org/wp-content/uploads/2015/08/flbcinc-360x230.png" alt="Family League of Baltimore" style="width:100px; height:100px;">
 +
</a>
 +
 
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                                      <a>
 +
  <img src="https://static.igem.org/mediawiki/2017/6/6c/T--Baltimore_Bio-Crew--fabian_kolker_small_icon.png" alt="Fabian Kolker Foundation" style="width:100px; height:100px;">
 +
</a>
 +
 
 +
<a href="http://vwrfoundation.org/">
 +
  <img src="https://static.igem.org/mediawiki/2016/1/1a/T--Baltimore_Biocrew--VWR_Foundation_LOGO.jpeg" alt="VWR Charitable Foundation" style="width:100px; height:100px;">
 +
</a>
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<a href="http://www.marylandrecyclingnetwork.org/">
 +
  <img src="https://media.licdn.com/mpr/mpr/shrink_200_200/AAEAAQAAAAAAAAI8AAAAJDY0ZDg0ZjlkLWVlMTItNGI1Mi1iNWEwLWYzMDVlYWMwMTZhZg.png" alt="Maryland Recycling Network" style="width:100px; height:100px;">
 +
</a>
 +
 
 +
<a href="https://www.rwdfoundation.org/">
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  <img src="https://static.igem.org/mediawiki/2016/6/65/T--Baltimore_BioCrew--DeutschFoundation.png" alt="The Robert W. Deutsch Foundation" style="width:100px; height:100px;">
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</a>
  
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</footer>
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</section>
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      </body>
 
</html>
 
</html>

Latest revision as of 19:44, 19 November 2017


BUGSS Logo
Home Team Project Results Design Notebook Composite Part Safety HP Silver Experiment Attribution Collaborations Interlab Contributions

Baltimore Bio-Crew

BALTIMORE BIO-CREW

Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor

Composite Parts

We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. The chlorogenate esterase construct (BBa_K2436001) was cloned successfully and submitted to the registry. The lipase construct was not cloned successfully. Our constructs were designed as follows.
Lactose Regulated Promoter (BBa_R0011): a DNA sequence that starts the transcription process in the presence of lactose or IPTG. Thus, the promoter is only active in certain times to regulate the amount of proteins the bacteria is able to produce at a time. The promoter causes the gene to activate and begin the process of forming mRNA and eventually proteins.
Ribosome Binding Site (BBa_B0030): an RNA sequence found in mRNA to which ribosomes can bind and initiate translation. It is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation.
PelB Secretion Tag (BBa_K2010002): allows for bacteria to secrete the enzymes (lipase and esterase) into the periplasm. Once the enzymes are secreted to the periplasm, they are able to exit the cell on their own.
Protein Coding Sequence (BBa_K2187001 for esterase or BBa_K2187000 for lipase): the DNA sequence is the lipase and esterase gene sequences. The lipase and esterase are both needed to successfully produce the plastic degrading enzymes because both work together to break down and eliminate the plastic particles. One construct contains the chlorogenate esterase coding sequence, and the other construct has the lipase coding sequence.
His Tag: allows us to purify the protein on a nickel column once it’s secreted outside of the bacteria
Terminator (BBa_K864600): a sequence that occurs at the end of the gene and causes transcription to stop