Difference between revisions of "Team:Northwestern"

 
(869 intermediate revisions by 5 users not shown)
Line 1: Line 1:
 +
{{Northwestern_Page_Head_temp}}
 +
{{Navbar_temp}}
  
−
<html lang="en">
+
<html>
−
<head>
+
<style>
−
  <meta charset="utf-8">
+
p.big {
−
  <meta http-equiv="X-UA-Compatible" content="IE=edge">
+
    line-height: 1.8;
−
  <meta name="viewport"content="width=device-width, initial-scale=1">
+
}
−
  <title>WikiSetup</title>
+
</style>
−
  <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:Northwestern/css/bootstrap?action=raw&ctype=text/css">
+
<head>  
−
  <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:Northwestern/css/custom?action=raw&ctype=text/css">
+
−
  <link rel="stylesheet" type="text/css" href="https://fonts.googleapis.com/css?family=Montserrat:400,700?action=raw&ctype=text/css">
+
−
  <style type='text/css'>
+
−
        ul.nav li.dropdown:hover ul.dropdown-menu {
+
−
            display: block;
+
−
        }
+
−
    </style>
+
−
<!--[if lt IE 9]>
+
−
  <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
−
  <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
−
  <![endif]-->  
+
  
  
 +
<meta charset="UTF-8">
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
    <title>Home</title>
 +
    <!-- Bootstrap -->
 +
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Northwestern/CSS?action=raw&ctype=text/css" />
 +
 +
<style>
 +
.foo img:last-child{display:none}
 +
.foo:hover img:first-child{display:none}
 +
.foo:hover img:last-child{display:inline-block}
 +
}
 +
 +
/*h2 {
 +
padding: 0;
 +
font-weight: 300;*/
 +
}
 +
h2 span {
 +
margin-left: 1em;
 +
color: #aaa;
 +
font-size: 85%;
 +
}
 +
 +
 +
</style>
 +
 +
 +
<style>
 +
button{border: 0; background-color: #D3D3D3
 +
}
 +
</style>
 
</head>
 
</head>
−
 
−
<body>
 
  
−
<nav class="navbar navbar-inverse navbar-static-top">
+
</style>
−
<div class="container-fluid">
+
 
−
<div class="navbar-header">
+
<script src="https://ajax.googleapis.com/ajax/libs/jquery/2.1.4/jquery.min.js"></script>
−
<button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
+
<script src="https://github.com/kswedberg/jquery-smooth-scroll/blob/master/jquery.smooth-scroll.min.js"></script>
−
<span class="sr-only">Toggle navigation</span>
+
<script>
−
<span class="icon-bar"></span>
+
$('.smooth').on('click', function() {
−
<span class="icon-bar"></span>
+
    $.smoothScroll({
−
</button>
+
        scrollElement: $('body'),
−
<a class="navbar-brand" href="">
+
        scrollTarget: '#' + this.id
−
<img alt="Brand" src="">
+
    });
−
</a>
+
   
−
</div>
+
    return false;
−
<div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
+
});
−
<ul class="nav navbar-nav navbar-right">
+
</script>
−
<li><a href="">Home</a></li>
+
<!-- First Parallax Image with Logo Text -->
−
<li><a href="">Mission</a></li>
+
<div class="w3-display-container w3-opacity-min" id="top">
−
<li><a href="">In the Lab</a></li>
+
  <div class="logo">
−
<li><a href="">About</a></li>
+
  </div>
−
<li class="dropdown">
+
<div style="z-index:1 !important;" class="w3-display-bottommiddle w3-hide-medium w3-hide-small">
−
<a href="">People<span class="caret"></span></a>
+
<button style="position:relative !important; z-index:1 !important;" class="icon-button" onclick="window.location.href='#first'">
−
<ul class="dropdown-menu">
+
    <span style="color:#4C177D;" class="w3-xxxlarge glyphicon glyphicon-chevron-down">
−
<li><a href="">Lulu Sum</a></li>
+
    </span>
−
<li><a href="">Jack Arnold</a></li>
+
 
−
<li><a href="">Ayesha Rahman</a></li>
+
 
−
<li><a href="">Will Reinhardt</a></li>
+
 
−
                                <li><a href="">Katerina Cheronis</a></li>
+
 
−
                                <li><a href="">Charley Rees</a></li>
+
 
−
                                <li><a href="">Karen Taylor</a></li>
+
  </button>
−
                                <li><a href="">Tyler Kramlich</a></li>
+
</div>
−
</ul>
+
</div>
−
</li>
+
 
−
     
+
 
−
</ul>
+
<div class="bgimg-2 w3-display-container" id="first">
 +
  <div style="padding: 5% 10%;">
 +
  <h3 style="color:white" class="w3-center w3-padding-large"><br><br>Engineering pathways for integrating functional Cas9 protein into OMVs </h3><br>  
 +
<p style="padding-right: 10%; padding-left:10%; font-size:14px; color:white;" class="big">  Inappropriate use of antibiotics has escalated the growing problem of antibiotic resistance in many threatening diseases. In 2014, the World Health Organization classified antibiotic resistance as a global epidemic. Inactivating resistance genes via Cas9 nuclease-mediated cleavage has been shown to be an effective means of combating this epidemic; however, methods of in vivo delivery are currently limited. Our team aims to deliver Cas9 to antibiotic-resistant, pathogenic bacteria through submicron bacterial outer membrane vesicles (OMVs) as a companion re-sensitization therapeutic to antibiotic treatment. OMVs are naturally produced by all Gram-negative bacteria and are used for crosstalk, stress responses, and nutrient acquisition. Their ability to be modified and directed with relative ease makes them an ideal carrier of CRISPR-Cas9. Aiding conventional antibiotic treatment, our technology will model a complete protein delivery system and transport functional Cas9 to target cells.<br><br>
 +
</p>
 +
 
 +
  <ul class="ch-grid" height="50%" style=padding:0;">
 +
    <li>
 +
      <div class="ch-item ch-img-2">
 +
        <div class="ch-info">
 +
          <h3 class="ch-bubble">About us</h3>
 +
          <p class="ch-bubble">Northwestern University iGEM<br>
 +
          <a class="ch-bubble" href="https://2017.igem.org/Team:Northwestern/Team#">LEARN MORE</a></p>
 +
        </div>
 +
      </div>
 +
    </li>
 +
    <li>
 +
      <div class="ch-item ch-img-1">
 +
        <div class="ch-info">
 +
          <h3 class="ch-bubble">Project description</h3>
 +
          <p class="ch-bubble">Outer Membrane Vesicles<br>
 +
          <a class="ch-bubble" href="https://2017.igem.org/Team:Northwestern/project">LEARN MORE</a></p>
 +
        </div>
 +
      </div>
 +
    </li>
 +
    <li>
 +
      <div class="ch-item ch-img-3">
 +
        <div class="ch-info">
 +
          <h3 class="ch-bubble">Parts</h3>
 +
          <p class="ch-bubble">Cas9 fusion proteins<br>
 +
            <a class="ch-bubble" href="https://2017.igem.org/Team:Northwestern/Parts">LEARN MORE</a></p>
 +
          </div>
 +
      </div>
 +
    </li>
 +
  </ul>
 +
 
 +
 
 +
</div>
 +
<div style="z-index:1 !important;" class="w3-display-bottommiddle">
 +
<button height="80%" width="auto" style="position:relative !important; z-index:1 !important;" class="icon-button w3-hide-small w3-hide-medium" onclick="scrollWin2()">
 +
    <span style="color:white;" class="w3-xxxlarge glyphicon glyphicon-chevron-down">
 +
    </span>
 +
  </button>
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
<div class="bgimg-3 w3-display-container" id="second">
 +
<div style="padding: 5% 10%;">
 +
<h3 style="black;" class="w3-center w3-padding-large"><br><br>Project significance</h3><br>  
 +
<p style="padding-bottom:0; padding-right: 10%; padding-left:10%; black; font-size:14px;" class="big"> VesiCure aims to create a therapeutic delivery system for CRISPR gene-editing technology using bacterial outer-membrane vesicles (OMVs). We hope to be able to utilize this delivery system to target the antibiotic resistant genes in bacteria, thereby allowing antibiotic treatments to be effective by eliminating the adaptive ability of bacterial resistance. <b>By genetically altering the resistant bacteria, this treatment would help combat the problem of antibiotic resistance directly from the source</b>.<br><br> </p>
 +
 
 +
<figure>  
 +
<br><br><br>
 +
<center><img src="https://static.igem.org/mediawiki/2017/c/c0/T--Northwestern--GlobePractices.png" width="350px" border="0">
 +
</center></figure>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div style="z-index:1 !important;" class="w3-display-bottommiddle">
 +
<button style="margin:0; padding:0; position:relative !important; z-index:1 !important;" class="icon-button w3-hide-small w3-hide-medium" onclick="scrollWin3()">
 +
    <span style="color:black;" class="w3-xxxlarge glyphicon glyphicon-chevron-down">
 +
    </span>
 +
  </button>
 +
</div></p>
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
<div class="w3-display-container">
 +
<div>
 +
  <h3 class="w3-center w3-padding-large">Check out the video below to learn more about our project!</h3><br>
 +
</div>
 +
<div class="w3-center container">  
 +
<span class="w3-center"> <video class="w3-center" controls><source src= "https://static.igem.org/mediawiki/2017/6/60/T--Northwestern--videofast.mp4" type="video/mp4" type="video/mp4"></video> </span>  
 +
</div>  
 +
</div>
 +
    <!-- Hide this text on small devices -->
 +
</div>
 +
 
 +
<!-- Container (Team Section) -->
 +
 
 +
  <!-- Responsive Grid. Four columns on tablets, laptops and desktops. Will stack on mobile devices/small screens (100% width)
 +
 
 +
 
 +
 
 +
<!-- Modal for full size images on click-->
 +
<div id="modal01" class="w3-modal w3-black" onclick="this.style.display='none'">
 +
  <span class="w3-button w3-large w3-black w3-display-topright" title="Close Modal Image"><i class="fa fa-remove"></i></span>
 +
  <div class="w3-modal-content w3-animate-zoom w3-center w3-transparent w3-padding-64">
 +
    <img id="img01" class="w3-image">
 +
    <p id="caption" class="w3-opacity w3-large"></p>
 +
  </div>
 
</div>
 
</div>
−
</div>
+
<br>
−
</nav>
+
<br>
−
+
−
<h1>TEST</h1>  
+
  
 
</body>
 
</body>
−
<div class="navbar navbar-inverse navbar-fixed-bottom">
 
−
<ul class="navbar-brand"><span class="glyphicon glyphicon-copyright-mark"></span>2017 Northwestern iGEM</ul> </div>
 
 
</html>
 
</html>
 +
{{Northwestern_Page_Foot_temp}}

Latest revision as of 17:09, 20 November 2017

Northwestern Template Northwestern Template

Overview Silver Gold Collaborations Engagement
Overview
Experiments Modeling Results Notebook Safety Interlab
Description Design Medal Criteria
About Us Attributions

Home



Engineering pathways for integrating functional Cas9 protein into OMVs


Inappropriate use of antibiotics has escalated the growing problem of antibiotic resistance in many threatening diseases. In 2014, the World Health Organization classified antibiotic resistance as a global epidemic. Inactivating resistance genes via Cas9 nuclease-mediated cleavage has been shown to be an effective means of combating this epidemic; however, methods of in vivo delivery are currently limited. Our team aims to deliver Cas9 to antibiotic-resistant, pathogenic bacteria through submicron bacterial outer membrane vesicles (OMVs) as a companion re-sensitization therapeutic to antibiotic treatment. OMVs are naturally produced by all Gram-negative bacteria and are used for crosstalk, stress responses, and nutrient acquisition. Their ability to be modified and directed with relative ease makes them an ideal carrier of CRISPR-Cas9. Aiding conventional antibiotic treatment, our technology will model a complete protein delivery system and transport functional Cas9 to target cells.



Project significance


VesiCure aims to create a therapeutic delivery system for CRISPR gene-editing technology using bacterial outer-membrane vesicles (OMVs). We hope to be able to utilize this delivery system to target the antibiotic resistant genes in bacteria, thereby allowing antibiotic treatments to be effective by eliminating the adaptive ability of bacterial resistance. By genetically altering the resistant bacteria, this treatment would help combat the problem of antibiotic resistance directly from the source.




Check out the video below to learn more about our project!