Difference between revisions of "Team:WLC-Milwaukee"

 
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<div class="container-fluid">
 
<div class="container-fluid">
  
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<img id="gauge" src="https://static.igem.org/mediawiki/2017/a/a9/T--WLC-Milwaukee--Phage_Gauge_4.gif">
  
  
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<img id="infographic" src="https://static.igem.org/mediawiki/2017/9/94/T--WLC-Milwaukee--Main_Page_Infographic_7_800px.png">
<h1> Welcome to iGEM 2017! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</div>  
 
</div>  
  
<div class="column full_size" >
 
<h1> Project Description </h1>
 
<p>Using Lambda Phage tails, the WLC iGEM team plans to develop a novel way to detect E. coli in water samples. Lambda Phage is an extremely well-studied and highly specific bacteriophage that binds to LamB, an outer membrane protein in E. coli. For our detection method, only the tail portion, which is the necessary component for LamB recognition is used. Our “Phage Gauge” will involve a purified phage tail solution conjugated to an enzyme that will act as a color indicator.  The phage tail will bind to LamB in E. coli cells, and require low CFU/mL for clear results. With a range of color brightness, our test will be quantitative in nature and provide a solution to the strictly qualitative tests on the market. Because the test will yield results in minutes, it can compete with already marketed tests. </p>
 
</div>
 
 
<div class="clear"></div>
 
 
<div class="column half_size" >
 
<h5>Before you start: </h5>
 
<p> Please read the following pages:</p>
 
<ul>
 
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
</ul>
 
</div>
 
 
<div class="column half_size" >
 
<div class="highlight">
 
<h5> Styling your wiki </h5>
 
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
</div>
 
</div>
 
 
<div class="column full_size" >
 
<h5> Wiki template information </h5>
 
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
 
</div>
 
 
 
 
 
<div class="column half_size" >
 
<h5> Editing your wiki </h5>
 
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
 
</div>
 
 
 
<div class="column half_size" >
 
<h5>Tips</h5>
 
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
<ul>
 
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
<li>Be clear about what you are doing and how you plan to do this.</li>
 
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
 
<li>Have lots of fun! </li>
 
</ul>
 
</div>
 
 
 
<div class="column half_size" >
 
<h5>Inspiration</h5>
 
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
<ul>
 
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
</ul>
 
</div>
 
 
<div class="column half_size" >
 
<h5> Uploading pictures and files </h5>
 
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
 
 
<a href="https://2017.igem.org/Special:Upload">
 
UPLOAD FILES
 
</a>
 
</p>
 
</div>
 
 
 
 
 
 
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<div class="main">
 
<div class="main">
<img id="infographic" src="https://static.igem.org/mediawiki/2017/9/99/T--WLC-Milwaukee--Main_Page_Infographic_1.png">
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<p id="text"><span class="bold medium">Using Lambda bacteriophage tails,</span> the WLC-Milwaukee iGEM team plans to develop a novel way to detect <i>Escherichia coli</i> in water samples. Lambda phage is an extremely well studied and highly specific bacteriophage that binds to LamB, an outer membrane protein in <i>E. coli</i>. For our detection method we use the
 +
J protein that resides in the tail region, which is a necessary component for LamB recognition. Our “Phage Gauge” will involve a purified phage tail solution conjugated to an enzyme that will act as a color indicator.  The phage tail will bind to LamB in <i>E. coli</i> cells, and allow detection of <i>E. coli</i> at low concentrations. With a range of color brightness, our test will be quantitative in nature and provide a solution to the largely qualitative tests on the market. Because the test will yield results in minutes, it can compete with already marketed tests. Our testing method, while currently specific to <i>E. coli</i> could be applied and used to detect other bacterial strains based on the bacteriophage tail protein used. </p>
  
 
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<a class="tile" href="https://2017.igem.org/Team:WLC-Milwaukee/Notebook"> <!-- Timeline or Parts or Judging?-->
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<a class="tile" href="https://2017.igem.org/Team:WLC-Milwaukee/Notebook">
<img src="https://static.igem.org/mediawiki/2017/7/72/T--WLC-Milwaukee--Documentation_Tile_Med.jpeg">
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<img src="https://static.igem.org/mediawiki/2017/0/04/T--WLC-Milwaukee--Documentation_Tile_Laura_Med.jpg">
 
<h2>Documentation</h2>
 
<h2>Documentation</h2>
 
</a>
 
</a>

Latest revision as of 00:00, 21 November 2017

Using Lambda bacteriophage tails, the WLC-Milwaukee iGEM team plans to develop a novel way to detect Escherichia coli in water samples. Lambda phage is an extremely well studied and highly specific bacteriophage that binds to LamB, an outer membrane protein in E. coli. For our detection method we use the J protein that resides in the tail region, which is a necessary component for LamB recognition. Our “Phage Gauge” will involve a purified phage tail solution conjugated to an enzyme that will act as a color indicator. The phage tail will bind to LamB in E. coli cells, and allow detection of E. coli at low concentrations. With a range of color brightness, our test will be quantitative in nature and provide a solution to the largely qualitative tests on the market. Because the test will yield results in minutes, it can compete with already marketed tests. Our testing method, while currently specific to E. coli could be applied and used to detect other bacterial strains based on the bacteriophage tail protein used.