Difference between revisions of "Team:WLC-Milwaukee/Description"

 
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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h1>Description</h1>
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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<h2 class="alt">Step one</h2>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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Phage tail tip conjugated
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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to a colorometric enzyme is added to a water sample by drawing water into the syringe. The tail tip recognizes LamB, an outer membrane protein on <i>E. coli</i> and binds to any <i>E. coli</i> present in the sample.  
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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<h2 class="alt">Step two</h2>
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<p class="big">The solution is syringe filtered through a 0.45 micrometer filter to trap bacteria bound to phage tails and allow any unbound phage tail through. This is followed by a series of washes which prevents false positives by ridding the solution of excess phage tails.
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<h2 class="alt">Step three</h2>
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<p class="big">Substrate is added to the filter which will be cleaved by the enzyme. Color change occurs if <i>E. coli</i> is present. Note, each <i>E. coli</i> cell can bind hundreds of phage tail and each tail may bind several enzyme, which allows for high sensitivity. </p>
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Latest revision as of 00:05, 21 November 2017

Description

Step one

Phage tail tip conjugated to a colorometric enzyme is added to a water sample by drawing water into the syringe. The tail tip recognizes LamB, an outer membrane protein on E. coli and binds to any E. coli present in the sample.

Step two

The solution is syringe filtered through a 0.45 micrometer filter to trap bacteria bound to phage tails and allow any unbound phage tail through. This is followed by a series of washes which prevents false positives by ridding the solution of excess phage tails.

Step three

Substrate is added to the filter which will be cleaved by the enzyme. Color change occurs if E. coli is present. Note, each E. coli cell can bind hundreds of phage tail and each tail may bind several enzyme, which allows for high sensitivity.