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<td><b>April 2016</b></td> | <td><b>April 2016</b></td> | ||
− | <td> | + | <td>Settled of lab duties and poitions <br> Lab safety Training<br> |
− | Started regular meetings every week | + | Started to have regular meetings every week |
</td> | </td> | ||
<td rowspan="4">N/A</td> | <td rowspan="4">N/A</td> | ||
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<td><b>May 2016</b></td> | <td><b>May 2016</b></td> | ||
− | <td rowspan="4">Recruitment of | + | <td rowspan="4">Recruitment of projects and prepare preliminary data for selection. Project related to pancreatic cancer diagnosis by Toehold Switch is selected</td> |
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Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout. | Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout. | ||
</td> | </td> | ||
− | <td> Attended | + | <td> Attended The 4th iGEM Asia-Pacific iGEM Conference </td> |
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<td><b>September 2016</b></td> | <td><b>September 2016</b></td> | ||
<td>Identified | <td>Identified | ||
− | problems | + | problems in designing Toehold Switch manually and outline programme functions for semi-automatic toehold switch design. |
</td> | </td> | ||
− | <td> | + | <td> Held exhibition and questionnaire related with synthetic biology in CUHK information day and reached secondary school students in Hong Kong. </td> |
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<td><b>November 2016</b></td> | <td><b>November 2016</b></td> | ||
− | <td rowspan="4"> | + | <td rowspan="4">Brainstormed and did literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch. </td> |
<td rowspan="4"> | <td rowspan="4"> | ||
Constructed programs which embedded with Influenza A sequence database and Vienna RNA package. | Constructed programs which embedded with Influenza A sequence database and Vienna RNA package. | ||
<br><br> | <br><br> | ||
− | Failed to test | + | Failed to test in cell free system due to insufficient materials and resources. |
<br><br> | <br><br> | ||
− | T7- | + | T7-RNA-polymerase amplification system is cloning to yield higher “ON/OFF”ratio.</td> |
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<td><b>December 2016</b></td> | <td><b>December 2016</b></td> | ||
<td rowspan="2">Requested for sponsorship.<br><br> | <td rowspan="2">Requested for sponsorship.<br><br> | ||
− | + | Skype with the Pakistan iGEM team to explore the potential of collaboration. | |
− | + | ||
− | + | ||
</td> | </td> | ||
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<td><b>July 2017</b></td> | <td><b>July 2017</b></td> | ||
<td></td> | <td></td> | ||
− | <td rowspan="2"> | + | <td rowspan="2">Cloned of influenza switch and trigger into our standard backbone <br> |
− | + | Validated switch by co-transformation<br> | |
− | + | Clond switch into shipping backbone</td> | |
<td></td> | <td></td> | ||
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<td><b>September 2017</b></td> | <td><b>September 2017</b></td> | ||
<td>.</td> | <td>.</td> | ||
− | <td>Mentored UCCKE high school team by providing advice on their project, troubleshooting routine lab | + | <td>Mentored UCCKE high school team by providing advice on their project, troubleshooting routine lab problems and also helped them to analysis data.</td> |
<td>Hardware prototype design </td> | <td>Hardware prototype design </td> | ||
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<td><b>October 2017</b></td> | <td><b>October 2017</b></td> | ||
<td></td> | <td></td> | ||
− | <td></td> | + | <td>Sub-cloned the switches and trigger to standard backbones</td> |
<td>Draft the hardware model and kit for Dr. Switch.</td> | <td>Draft the hardware model and kit for Dr. Switch.</td> | ||
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Latest revision as of 03:45, 22 November 2017