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+ | |||
+ | |||
+ | |||
+ | <section id="section01"> | ||
+ | <div class="splash" id="mainsplash"> | ||
+ | <div id="banner"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d5/Notebook_banner2.png" width="100%" height="auto" class=" igem-logo"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | <section id="section02"> | ||
+ | <table width="100%"> | ||
+ | |||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th>Administrative Work</th> | ||
+ | <th> Laboratory Work</th> | ||
+ | <th>Human Practice</th> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>April 2016</b></td> | ||
+ | <td>Settled of lab duties and poitions <br> Lab safety Training<br> | ||
+ | Started to have regular meetings every week | ||
+ | </td> | ||
+ | <td rowspan="4">N/A</td> | ||
+ | <td rowspan="4">N/A</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>May 2016</b></td> | ||
+ | <td rowspan="4">Recruitment of projects and prepare preliminary data for selection. Project related to pancreatic cancer diagnosis by Toehold Switch is selected</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>June 2016</b></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>July 2016</b></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>August 2016</b></td> | ||
+ | <td rowspan="3"> | ||
+ | Cloning of switches and its | ||
+ | corresponding triggers into backbones with significant “ON/OFF”ratio in vivo. | ||
+ | <br><br> | ||
+ | Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout. | ||
+ | </td> | ||
+ | <td> Attended The 4th iGEM Asia-Pacific iGEM Conference </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>September 2016</b></td> | ||
+ | <td>Identified | ||
+ | problems in designing Toehold Switch manually and outline programme functions for semi-automatic toehold switch design. | ||
+ | </td> | ||
+ | <td> Held exhibition and questionnaire related with synthetic biology in CUHK information day and reached secondary school students in Hong Kong. </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>October 2016</b></td> | ||
+ | <td>Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A.</td> | ||
+ | <td rowspan="2">Request for sponsorship.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>November 2016</b></td> | ||
+ | <td rowspan="4">Brainstormed and did literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch. </td> | ||
+ | <td rowspan="4"> | ||
+ | Constructed programs which embedded with Influenza A sequence database and Vienna RNA package. | ||
+ | <br><br> | ||
+ | Failed to test in cell free system due to insufficient materials and resources. | ||
+ | <br><br> | ||
+ | T7-RNA-polymerase amplification system is cloning to yield higher “ON/OFF”ratio.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><b>December 2016</b></td> | ||
+ | <td rowspan="2">Requested for sponsorship.<br><br> | ||
+ | Skype with the Pakistan iGEM team to explore the potential of collaboration. | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>January 2017</b></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>February 2017</b></td> | ||
+ | <td>Organised day camp, “One Day Biochemist”, for secondary school students to try out working in standard laboratory.</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>March 2017</b></td> | ||
+ | <td>Completed individual registration for iGEM 2018</td> | ||
+ | <td rowspan="2">Successful cloning of standard backbone, which allows standardized toehold switch cloning with less false positive results. | ||
+ | |||
+ | Unsuccessful cloning of T7-rna-polymerase amplification system. | ||
+ | </td> | ||
+ | <td>Meeting with Joseph Sung, who is a medical expert and Vice Chancellor of CUHK.</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>April 2017</b></td> | ||
+ | <td></td> | ||
+ | <td> | ||
+ | Designed the product construction of Toehold Switch and outlined the prototype (user manual). | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>May 2017</b></td> | ||
+ | <td>Reviewed the updated metal requirement and revised the division of work</td> | ||
+ | <td rowspan="2"> | ||
+ | Continued cloning Toehold switch for Influenza A subtyping. | ||
+ | |||
+ | Characterization on ability of fluorescent proteins by cloning amajLime and mRFP to pSB1A2 and pSB1C3 with constitutive promoter J23100 under different pH environment. | ||
+ | |||
+ | </td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>June 2017</b></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>July 2017</b></td> | ||
+ | <td></td> | ||
+ | <td rowspan="2">Cloned of influenza switch and trigger into our standard backbone <br> | ||
+ | Validated switch by co-transformation<br> | ||
+ | Clond switch into shipping backbone</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>August 2017</b></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>September 2017</b></td> | ||
+ | <td>.</td> | ||
+ | <td>Mentored UCCKE high school team by providing advice on their project, troubleshooting routine lab problems and also helped them to analysis data.</td> | ||
+ | <td>Hardware prototype design </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><b>October 2017</b></td> | ||
+ | <td></td> | ||
+ | <td>Sub-cloned the switches and trigger to standard backbones</td> | ||
+ | <td>Draft the hardware model and kit for Dr. Switch.</td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
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+ | {{Template:Hong_Kong-CUHK_footer}} |
Latest revision as of 03:45, 22 November 2017