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− | <h2>Attributions | + | <h2>Attributions</h2> |
− | <p> | + | <p></p> |
− | < | + | <p>In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.</p> |
− | Preparation of electro-competent S. | + | <p> |
+ | <ul class="ulclass"> | ||
+ | <li>• <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?sprache_in=en&menue_id_in=101&id_in=441" target="_blank">Diethard Mattanovich,</a> <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?sprache_in=en&ansicht_in=&menue_id_in=101&id_in=1307" target="_blank">Brigitte Gasser,</a> <a href="https://forschung.boku.ac.at/fis/suchen.person_uebersicht?id_in=5812&menue_id_in=101&sprache_in=de" target="_blank"> Michael Sauer</a>and Hans Marx: organization of the iGEM project | ||
+ | </li><li>• Gabi Wilt ordered materials for our team | ||
+ | </li><li>• Corinna Rebnegger helped us doing flow cytometric experiments for characterization of our pGAL syn | ||
+ | </li><li>• Tom Gaßler advice on setting up our CRISPR/Cas9 mediated gene integration in <i> S. cerevisiae </i> by supporting us with primers for assembling the specific sgRNAs <nobr> (adapted from <a href="https://doi.org/10.1016/j.meteno.2015.03.001" target="_blank">Stovicek 2015).</a></nobr> | ||
+ | </li><li>• Martin Altvater: provided us with different plasmids for <i> Saccharomyces cerevisiae </i> ready for our Golden Gate system | ||
+ | </li><li>• Jürgen Zanghellini supported us with modeling | ||
+ | </li><li>• Florian Strobl advice and lab material | ||
+ | </li><li>• Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids | ||
+ | </li><li>• Franziska Doleschal organized our bank account and managed all our invoices | ||
+ | </li><li>• Anna Gindl designed our Logo | ||
+ | </li><li>• Nikolaus Huber helped us setting up the core code of our wiki page | ||
+ | </li><li>• Sauer group provided us BB1_01 – BB1_08 | ||
+ | </li><li>• Mattanovich/ Gasser group provided us material, all empty BBs for the Golden Gate system and many protocols: | ||
+ | <ul class="ulclass"> | ||
+ | <li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#CompetentColi">Preparation chemical competent <i>E. coli</i></a> | ||
+ | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationColi">Transformation chemical competent <i>E. coli</i></a> | ||
+ | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#PreparationYeast">Preparation of electro competent <i>S. cerevisiae</i></a> | ||
+ | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationYeast">Transformation of electro competent <i>S. cerevisiae</i></a> | ||
+ | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#mRNA">mRNA extraction</li></ul></li></a> | ||
+ | <li>• Bernd Albrecht helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters. | ||
+ | </li><li> • STV LBT helped us organizing the "Spritzerstand"</li></ul> | ||
+ | </p> | ||
+ | <p> Furthermore, we want to thank Matthias Steiger, Alexandra Graf, Stefanie Wiesauer, Christiane Veit, Gordana Wozniak-Knopp, Siniša Petrik, Roland Prielhofer, Hannes Rußmayer, Michael Egermeier, Valeria Ellena, Lina Heistinger, Dominik Jeschek, Richard Zahrl, Ursula Kieswetter and many more!</p> | ||
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Latest revision as of 13:10, 22 November 2017
Attributions
In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.
- • Diethard Mattanovich, Brigitte Gasser, Michael Sauerand Hans Marx: organization of the iGEM project
- • Gabi Wilt ordered materials for our team
- • Corinna Rebnegger helped us doing flow cytometric experiments for characterization of our pGAL syn
- • Tom Gaßler advice on setting up our CRISPR/Cas9 mediated gene integration in S. cerevisiae by supporting us with primers for assembling the specific sgRNAs
(adapted from Stovicek 2015). - • Martin Altvater: provided us with different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
- • Jürgen Zanghellini supported us with modeling
- • Florian Strobl advice and lab material
- • Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
- • Franziska Doleschal organized our bank account and managed all our invoices
- • Anna Gindl designed our Logo
- • Nikolaus Huber helped us setting up the core code of our wiki page
- • Sauer group provided us BB1_01 – BB1_08
- • Mattanovich/ Gasser group provided us material, all empty BBs for the Golden Gate system and many protocols:
- • Bernd Albrecht helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.
- • STV LBT helped us organizing the "Spritzerstand"
Furthermore, we want to thank Matthias Steiger, Alexandra Graf, Stefanie Wiesauer, Christiane Veit, Gordana Wozniak-Knopp, Siniša Petrik, Roland Prielhofer, Hannes Rußmayer, Michael Egermeier, Valeria Ellena, Lina Heistinger, Dominik Jeschek, Richard Zahrl, Ursula Kieswetter and many more!