Difference between revisions of "Team:Exeter/Applied Design"

 
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         <nav class="nav nav-pills flex-column">
 
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           <a class="nav-link" href="#h1">Our Motivations</a>
 
           <a class="nav-link" href="#h1">Our Motivations</a>
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           <a class="nav-link" href="#h2">Our Filtration System</a>
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           <a class="nav-link" href="#h2">Our Filtration <br> System</a>
 
           <a class="nav-link" href="#h3">Stakeholder <br> Collaboration</a>
 
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<h1 id="pageHeader"><title><centre> Applied Design</centre></title></h1>
 
<h1 id="pageHeader"><title><centre> Applied Design</centre></title></h1>
  
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<div style="width:90%;
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<div class="d-block mx-auto w-100">
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  <a href="https://2017.igem.org/Team:Exeter/Current_Methods">
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<b><p style="text-align:center"> Click on the buttons below to find out more about our applied design. </p></b>
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  <figure class="mx-auto float-left w-25">
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<br></br>
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  <img class="d-block w-75 mx-auto" src="https://static.igem.org/mediawiki/2017/8/85/T--Exeter--Current_Methods_Icon.png">
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<div style="height:auto ;margin:0 ;width:15%; float:left">
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  <figcaption style="text-align:center">
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<a href="https://2017.igem.org/Team:Exeter/Hydrocyclone">
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    Current Methods
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<figure style="float:center;">
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  </figcaption>
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  <img src="https://static.igem.org/mediawiki/2017/6/64/T--Exeter--Hydrocyclone.png" width="104" />
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  </figure>
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<figcaption>Hydrocyclone</figcaption>
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  </a>
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</figure>
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</a>
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<a href="https://2017.igem.org/Team:Exeter/Hydrocyclone">
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</div>
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<figure class="mx-auto float-left w-25">
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  <img class="d-block w-75 mx-auto"  src="https://static.igem.org/mediawiki/2017/6/64/T--Exeter--Hydrocyclone.png">
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  <figcaption style="text-align:center">
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    Hydrocyclone
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  </figcaption>
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</figure>
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</a>
  
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<div
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<a href="https://2017.igem.org/Team:Exeter/MBR">
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<div style="height:auto ;margin:0 ;width:15%; float:left">
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<figure class="mx-auto float-left w-25">
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<a href="https://2017.igem.org/Team:Exeter/FMR">
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  <img class="d-block w-75 mx-auto" src="https://static.igem.org/mediawiki/2017/4/49/T--Exeter--FMR_Icon.png">
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<figure style="float:center">
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  <figcaption style="text-align:center">
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  <img src="https://static.igem.org/mediawiki/2017/6/64/T--Exeter--Hydrocyclone.png" alt="https://static.igem.org/mediawiki/2017/8/8a/T--Exeter--GroupedGraphs_EcoliperunitA2.png" width="104" />
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    Metal Binding Reactor
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<figcaption>Fluidised Media Reactor</figcaption>
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  </figcaption>
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</figure>
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</figure>
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</a>
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</a>
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</div>
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<div style="height:auto ;margin:0 ;width:15%; float:left">
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<a href="https://2017.igem.org/Team:Exeter/Bio-security">
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<a href="https://2017.igem.org/Team:Exeter/Bio-security">
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  <figure class="mx-auto float-left w-25">
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<figure style="float:center">
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  <img class="d-block w-75 mx-auto" src="https://static.igem.org/mediawiki/2017/d/dc/T--Exeter--Biosecurity_Icon.png">
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  <img src="https://static.igem.org/mediawiki/2017/d/dc/T--Exeter--Biosecurity_Icon.png" width="104" />
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  <figcaption style="text-align:center">
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<figcaption>Biosecurity Mechanism</figcaption>
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    Biosecurity Mechanism
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</figure>
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  </figcaption>
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</a>
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  </figure>
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</div>
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  </a>
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</div>
  
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<div style="height:auto ;margin:0 ;width:15%; float:left">
 
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<a href="https://2017.igem.org/Team:Exeter/Current_Methods">
 
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<figure style="float:center">
 
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  <img src="https://static.igem.org/mediawiki/2017/d/dc/T--Exeter--Biosecurity_Icon.png" width="104" />
 
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<figcaption>Current Methods</figcaption>
 
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</figure>
 
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</a>
 
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</div>
 
  
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<div style="height:auto ;margin:0 ;width:15%; float:left">
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<div class="d-block mx-auto w-50">
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<a href="https://2017.igem.org/Team:Exeter/Filter_Stakeholders">
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<a href="https://2017.igem.org/Team:Exeter/Filter_Stakeholders">
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<figure style="float:center">
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<figure class="mx-auto float-left w-50">
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  <img src="https://static.igem.org/mediawiki/2017/d/dc/T--Exeter--Biosecurity_Icon.png" width="104" />
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  <img class="d-block w-75 mx-auto"  src="https://static.igem.org/mediawiki/2017/5/5a/T--Exeter--Human_Practices_Icon.png">
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<figcaption>Stakeholders' Influence</figcaption>
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  <figcaption style="text-align:center">
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</figure>
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    Stakeholders' Influence
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</a>
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  </figcaption>
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</div>
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</figure>
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</a>
  
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<div style="height:auto ;margin:0 ;width:15%; float:left">
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<a href="https://2017.igem.org/Team:Exeter/Future">
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<a href="https://2017.igem.org/Team:Exeter/Future">
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<figure class="mx-auto float-left w-50">
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<figure style="float:center">
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  <img class="d-block w-75 mx-auto" src="https://static.igem.org/mediawiki/2017/7/77/T--Exeter--Filter_Future_Icon.png">
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  <img src="https://static.igem.org/mediawiki/2017/7/77/T--Exeter--Filter_Future_Icon.png" width="104" />
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  <figcaption style="text-align:center">
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<figcaption>Future</figcaption>
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    Future
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</figure>
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  </figcaption>
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</figure>
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</a>
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<div style="width=90%; clear:left; margin:30px auto">
 
<div style="width=90%; clear:left; margin:30px auto">
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<h3 id="h1">Our Motivations</h3>
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<h2 id="h1">Our Motivations</h2>
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<p>The pollution of heavy metal ions in the South West, as a result of the area historic and prolific mining industry,  
+
<p>The pollution of heavy metal ions in the South West, as a result of the areas historic and prolific mining industry,  
 
  is a significant problem due to leaching of the metal ions. Such leaching has serious consequential impacts on the surrounding ecology and on  
 
  is a significant problem due to leaching of the metal ions. Such leaching has serious consequential impacts on the surrounding ecology and on  
 
  human health. Motivated to find a solution our team at Exeter have spent the summer working on developing a cost effective and sustainable solution that will have a reduced effect on the environment compared to the existing technologies in use. This page is an introduction to how we have <b>designed</b>, <b>engineered</b> and <b>experimentally tested</b> a novel filtration system for the bio-remediation of water contaminated with heavy metal ions. </p>
 
  human health. Motivated to find a solution our team at Exeter have spent the summer working on developing a cost effective and sustainable solution that will have a reduced effect on the environment compared to the existing technologies in use. This page is an introduction to how we have <b>designed</b>, <b>engineered</b> and <b>experimentally tested</b> a novel filtration system for the bio-remediation of water contaminated with heavy metal ions. </p>
 +
 
<figure>
 
<figure>
 
   <img class="rounded mx-auto d-block w-50" src="https://static.igem.org/mediawiki/2017/5/50/T--Exeter--wheal_maid_background2.jpeg">
 
   <img class="rounded mx-auto d-block w-50" src="https://static.igem.org/mediawiki/2017/5/50/T--Exeter--wheal_maid_background2.jpeg">
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<figcaption>Figure 1: Wheal Maid Lagoon</figcaption>
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<figcaption><center><b>Figure 1:</b> Wheal Maid Lagoon</center></figcaption>
 
</figure>
 
</figure>
 
</p>
 
</p>
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<p><h4><I>"Areas affected by contaminated mine water are often damaged for many decades, if not centuries. Because the treatment of polluted mine water is usually expensive, active treatment is usually used in heavily populated areas, at working mines, or where governmental money was made available for treatment purposes." (Wolkersdorfer, C. 2008)</i></h4></p>
 
<p><h4><I>"Areas affected by contaminated mine water are often damaged for many decades, if not centuries. Because the treatment of polluted mine water is usually expensive, active treatment is usually used in heavily populated areas, at working mines, or where governmental money was made available for treatment purposes." (Wolkersdorfer, C. 2008)</i></h4></p>
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<h3 id="h2">Our Filtration System</h3>
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<h2 id="h2">Our Filtration System</h2>
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  <p> Many of the existing treatment methods currently employed to deal with the contamination are expensive and often produce tonnes of hazardous waste each year. The Pili Plus filtration system has been developed to provide a cheaper more environmentally conscious alternative to these methods based on  
+
  <p> Many of the existing treatment methods currently employed to deal with the contamination are expensive and often produce tonnes of hazardous waste each year. The Pili<sup>+</sup> filtration system has been developed to provide a cheaper more environmentally conscious alternative to these methods based on  
−
  the use of our re-purposed adhesion mechanism of <i>E.coli</i>. Additionally, the filtration system is modular meaning that each component is independent therefore they can be adapted or interchanged with another component that may be potentially more suited to the situation that the filtration system is being used in. For example if an alternative biosecurity mechanism is required, potentially due to materials available or limited funds, then since it is not incorporated directly within the other components this would be feasible to change.</p>
+
  the use of our re-purposed adhesion mechanism of <i>E. coli</i>. Additionally, the filtration system is modular meaning that each component is independent therefore they can be adapted or interchanged with another component that may be potentially more suited to the situation that the filtration system is being used in. For example if an alternative biosecurity mechanism is required, potentially due to materials available or limited funds, then since it is not incorporated directly within the other components this would be feasible to change.</p>
 
   
 
   
 
<h4> Our novel filtration system is comprised of three modular parts: </h4>
 
<h4> Our novel filtration system is comprised of three modular parts: </h4>
 
<div style="border:20px 0 20px 0">
 
<div style="border:20px 0 20px 0">
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<div class="d-block mx-auto w-100">
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<video  controls>
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<video  class="mx-auto d-block" controls autoplay loop>
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  <source src="https://static.igem.org/mediawiki/2017/7/7d/T--Exeter--Filter_Animation.mp4" type="video/mp4">
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        <source src="https://static.igem.org/mediawiki/2017/7/7d/T--Exeter--Filter_Animation.mp4" type="video/mp4">
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Your browser does not support the video tag.
 
Your browser does not support the video tag.
 
</video>
 
</video>
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<ul>
 
<ul>
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<li>The first component is a <b>Hydrocyclone</b> this is used to separate sediment from the water.</li>
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<li>The first component is a <b>Hydrocyclone</b> this is used to separate sediment from the water. The video, shows a sediment particle (brown) gets separated, while the metal ions (pink) do not get separated and flows up the inner vortex with the water.</li>
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<li>The second component is a <b>Fluidised Media Reactor</b> which houses the genetically modified <i>E. coli</i>. This is where the metal ions bind to the pili.</li>
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<li>The second component is a <b>Metal Binding Reactor</b> which houses the genetically modified <i>E. coli</i>. This is where the metal ions (pink) bind to the pili.</li>
 
<li>The third component is a <b>Biosecurity Mechanism</b> which is used to kill the <i>E. coli</i> to prevent the genetically modified organisms from escaping into the environment.</li>
 
<li>The third component is a <b>Biosecurity Mechanism</b> which is used to kill the <i>E. coli</i> to prevent the genetically modified organisms from escaping into the environment.</li>
 
</ul>
 
</ul>
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<br></br>
 
<br></br>
 
   
 
   
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  <h3 id="h3"> Stakeholder Collaboration</h3>
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  <h2 id="h3"> Stakeholder Collaboration</h2>
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<p> We would like to thank the following stakeholder for sparing their time and offering invaluable suggestion and advice to help with the
+
<p> We would like to thank the following stakeholders for sparing their time and offering invaluable suggestions, advice and equipment to help with the
 
  progression of our filtration system. <a href="https://2017.igem.org/Team:Exeter/Filter_Stakeholders">Click here to find out more about our continual collaboration with these external stakeholders.</a></p>
 
  progression of our filtration system. <a href="https://2017.igem.org/Team:Exeter/Filter_Stakeholders">Click here to find out more about our continual collaboration with these external stakeholders.</a></p>
 
<img class="rounded mx-auto d-block w-50" src="https://static.igem.org/mediawiki/2017/6/65/T--Exeter--greenpeace_logo.png">
 
<img class="rounded mx-auto d-block w-50" src="https://static.igem.org/mediawiki/2017/6/65/T--Exeter--greenpeace_logo.png">

Latest revision as of 09:38, 26 November 2017

Introduction

<centre> Applied Design</centre>

Current Methods
Hydrocyclone
Metal Binding Reactor
Biosecurity Mechanism
Stakeholders' Influence
Future

Our Motivations

The pollution of heavy metal ions in the South West, as a result of the areas historic and prolific mining industry, is a significant problem due to leaching of the metal ions. Such leaching has serious consequential impacts on the surrounding ecology and on human health. Motivated to find a solution our team at Exeter have spent the summer working on developing a cost effective and sustainable solution that will have a reduced effect on the environment compared to the existing technologies in use. This page is an introduction to how we have designed, engineered and experimentally tested a novel filtration system for the bio-remediation of water contaminated with heavy metal ions.

Figure 1: Wheal Maid Lagoon

"Areas affected by contaminated mine water are often damaged for many decades, if not centuries. Because the treatment of polluted mine water is usually expensive, active treatment is usually used in heavily populated areas, at working mines, or where governmental money was made available for treatment purposes." (Wolkersdorfer, C. 2008)

Our Filtration System

Many of the existing treatment methods currently employed to deal with the contamination are expensive and often produce tonnes of hazardous waste each year. The Pili+ filtration system has been developed to provide a cheaper more environmentally conscious alternative to these methods based on the use of our re-purposed adhesion mechanism of E. coli. Additionally, the filtration system is modular meaning that each component is independent therefore they can be adapted or interchanged with another component that may be potentially more suited to the situation that the filtration system is being used in. For example if an alternative biosecurity mechanism is required, potentially due to materials available or limited funds, then since it is not incorporated directly within the other components this would be feasible to change.

Our novel filtration system is comprised of three modular parts:

  • The first component is a Hydrocyclone this is used to separate sediment from the water. The video, shows a sediment particle (brown) gets separated, while the metal ions (pink) do not get separated and flows up the inner vortex with the water.
  • The second component is a Metal Binding Reactor which houses the genetically modified E. coli. This is where the metal ions (pink) bind to the pili.
  • The third component is a Biosecurity Mechanism which is used to kill the E. coli to prevent the genetically modified organisms from escaping into the environment.


Stakeholder Collaboration

We would like to thank the following stakeholders for sparing their time and offering invaluable suggestions, advice and equipment to help with the progression of our filtration system. Click here to find out more about our continual collaboration with these external stakeholders.

References

  • Wolkersdorfer, C., Chapter 11: Mine Water Treatment and Ground Water Protection in Water Management at Abandoned Flooded Underground Mines: Fundamentals, Tracer Tests, Modelling, Water Treatment, A. International Mine Water and I. ebrary, Editors. 2008, Springer: Berlin. p. 235.