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<p class="question">15. Does your project include the use of anti-microbial resistance factors? For more information see the Safety Policy page and the White List. </p>
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<p class="question">16. Does your project include the use of anti-microbial resistance factors? For more information see the Safety Policy page and the White List. </p>
 
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<!-------------------------------------------------------------------------------------------- Question 17  --------------------------------------------------------------------------------------------------------->
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<h3>Parts and organisms not on the Whitelist </h3>
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<p class="question">17. Does your project include parts or organisms not on the Whitelist?</p>
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<!-------------------------------------------------------------------------------------------- Question 18  --------------------------------------------------------------------------------------------------------->
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p class="question">18. What is your chassis organism?</p>
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<p>Check all species you are genetically modifying in your project.</p>
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<ul>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_ecoli"> E. coli (give names of all strains you are using):</label> <textarea rows="2" name="E. coli strain" data-form-field="chassis_ecoli_describe" data-validation="[data-form-field=chassis_ecoli]"></textarea></li>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_yeast"> Yeast (Saccharomyces)</label></li>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_lactobacillus"> Lactobacillus</label></li>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_bsubtilis"> B. subtilis</label></li>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_other"> Others (give species names): </label> <textarea rows="2" name="Other chassis (give species names)" data-form-field="chassis_other_describe" data-validation="[data-form-field=chassis_other]"></textarea></li>
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<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_none"> No chassis organism (please comment):</label> <textarea rows="2" name="No chassis (comment)" data-form-field="chassis_none_describe" data-validation="[data-form-field=chassis_none]"></textarea></li>
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</ul>
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<p class="question">Comments:</p>
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<textarea rows="2" name="Chassis organism -- comments" data-form-field="chassis_comments"></textarea>
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Revision as of 14:05, 29 March 2017

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Final Safety Form

This form is for you to tell us all about your project, the organisms/parts you are using, the potential risks of your project, and what you are doing to reduce those risks.

  • We encourage STUDENTS, instead of instructors, to complete this form. However, you will need an Instructor or PI to sign and submit this form.
  • While you type, this form will remember your answers. When you are finished, press the "Submit" button at the bottom to send your form to the iGEM Safety Committee.
  • Submit this form by September 1.
  • If you will not be able to complete this form before the deadline, please email us (safety AT igem DOT org) and tell us about your situation.

Safety Form

Please choose a team

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Contact Information

Name

Email

Note

It is your responsibility to demonstrate that your work is safe. The easiest way to do this is through a well-researched and comprehensive safety form.

About our Lab

This part of the form is for you to tell us about your laboratory space and your safety equipment. If you need help, please consult your faculty advisor or laboratory manager. You can also read the Safety page and the Risk Groups page for basic information about different types of biology labs.

1. Please upload a photo or two of your lab, preferably showing the relevant safety features and paste the link here:

Biosafety Level

2. What is the Safety Level of your lab? [Help about Risk Groups and Safety Levels]

iGEM teams should not use Risk Group 3 or 4 organisms, and they should not work in Safety Level 3 or 4 labs. If you are planning to work at Safety Level 3 or 4, contact safety (AT) igem (DOT) org right away!!

Work areas

3 Which work areas do you use to handle biological materials? Please check all that apply.

Biosafety Training

4. Have your team members received any safety training yet?

  • Yes, we have already received safety training.
  • We plan to receive safety training in the future:
  • We will not have safety training:

For the purposes of iGEM, biosafety and biosecurity training covers the procedures and practices used to manage risks to your team, colleagues and institution, communities and the environment - from accidents or deliberate misuse of your projects. All team members are expected to be aware of these risks and to work to manage them

5. Please briefly describe the topics that you learned about (or will learn about) in your safety training.

  • Lab access and rules (including appropriate clothing, eating and drinking, etc.)
  • Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)
  • Differences between biosafety levels
  • Biosafety equipment (such as biosafety cabinets)
  • Good microbial technique (such as lab practices)
  • Disinfection and sterilization
  • Emergency procedures
  • Transport rules
  • Chemicals, fire and electrical safety
  • Other

6. Who provided your safety training? Did you get training from multiple people?

  • University biosafety office
  • Departmental specialist
  • PIs/instructors
  • Other

Oversight

7. Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety? Please give a link to these guidelines, or briefly describe them if you cannot give a link.

8. In your country / region, what are the laws and regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.

Uncertainties

9. Are there any areas where you have encountered uncertainties concerning institutional, regional, national or international rules and regulations and whether they are relevant to your lab and/or work?

10. Do you think the design of current regulations is sufficient to ensure safe and ethical practices?

(If not, how else could you approach the design? We’re interested in your ideas for strategies that could be used to promote safe and ethical practices as it becomes easier to engineer biology (i.e. monitoring people or information, building safety into the design of equipment, etc). Can you think of any useful examples from other fields?)

About our Project

This part of the form is for you to tell us about your primary project idea, what organisms and parts you will use and what your project will do. We know that iGEM teams often change project topics during the summer. When you change your primary project idea, please update this form to tell us about your new idea! If you are still working on multiple project ideas by June 30, you may choose one to describe on this form, or you may tell us about other ideas in the "Further Comments" section.

Project Overview

11. How will your project work?

Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)

Future applications

12. How would your project be used in the real world?

Imagine that your project was fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes and expand in the comments section.


  • (Examples: library of standardized promoters, system for communication between cells)

  • (Examples: reporter strain for measuring the strength of promoters)

  • (Examples: cells that make a flavor chemical for food, cells that make biofuel)

  • (Examples: cells that clean your clothes, bread made with engineered yeast)

  • (Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)

  • (Examples: a bio-sensing strip with cells that detect arsenic)

  • (Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought)

  • (Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants)

  • (Examples: bacteria that live on Mars)

13. What safety or ethical risks would be involved with such a use?

Specific risks

14. Does your project include the use of gene drives?


  • STOP: Gene Drives are not allowed in iGEM projects without a special exception from the Safety Committee. For more information see the Safety Policy page and the White List. Please contact the Safety Committee by emailing safety AT igem DOT org

For the purposes of iGEM, a gene drive includes Cas9 (and other endonucleases, such as dCas9 and Cpf1) integrated into the genome (including through the use of gRNA) of a sexually reproducing eukaryotic organisms (including organisms that reproduce both sexually and asexually, such as yeast) and/or the use of a drive to impact the progeny.

15. Does your project include the use of animals?


  • STOP: The use of animals is not allowed in iGEM projects without a special exception from the Safety Committee. For more information see the Safety Policy page and the White List. Please contact the Safety Committee by emailing safety AT igem DOT org

16. Does your project include the use of anti-microbial resistance factors? For more information see the Safety Policy page and the White List.

  • If you cannot provide such a reference you will need to complete a check-in form for this part.
  • You will need to complete a check-in form for this part.

BOX: For the purposes of iGEM, anti-microbial resistance factors are genetic sequences, or any other factor, that can increase an organism's resistance to drugs or therapeutics used to control them. This includes genes known to confer resistance to antibiotics or antiviral drugs.

Parts and organisms not on the Whitelist

17. Does your project include parts or organisms not on the Whitelist?

p class="question">18. What is your chassis organism?

Check all species you are genetically modifying in your project.

Comments:

Submit

Only a team Instructor or PI may submit the Safety Form.

Instructors/PIs, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Final Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.

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