Difference between revisions of "Team:Toronto/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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<p>To our knowledge, this is the first time that a CRISPRi metabolite challenge assay has been applied both to test the growth of cells with CRISPRi system and to indirectly assay the binding of an anti-CRISPR protein to the pdCas9-sgRNA complex. The basis of this assay could be applied to numerous systems, especially cell culture where the phenotypes or survival of cells can be easily enumerated at different time points. In particular, we envision using this assay to interrogate the application of a CRISPR/anti-CRISPR switch to the treatment of metabolic diseases, as complex biochemical pathways can be easily halted or redirected by targeting certain enzymes.</p>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<p>iGEM Toronto has also created and demonstrated the utility of our light boxes by measuring the kinetics of our LacILOV-mCherry construct. In the future, these light boxes could be utilized by other teams to carefully regulate the intensity and duration of light pulses for other light-regulated synthetic biology constructs, in addition to reducing ambient light. To this end, we have created a guide to using these light boxes for CRISPR-Cas9 prokaryotic systems like our own, alongside a putative design for mammalian systems. Please see our guide page for more details.</p>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
 
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<h5> Standard teams </h5>
 
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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<h5> Special track teams </h5>
 
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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Latest revision as of 19:13, 7 December 2017

Demonstrate

To our knowledge, this is the first time that a CRISPRi metabolite challenge assay has been applied both to test the growth of cells with CRISPRi system and to indirectly assay the binding of an anti-CRISPR protein to the pdCas9-sgRNA complex. The basis of this assay could be applied to numerous systems, especially cell culture where the phenotypes or survival of cells can be easily enumerated at different time points. In particular, we envision using this assay to interrogate the application of a CRISPR/anti-CRISPR switch to the treatment of metabolic diseases, as complex biochemical pathways can be easily halted or redirected by targeting certain enzymes.

iGEM Toronto has also created and demonstrated the utility of our light boxes by measuring the kinetics of our LacILOV-mCherry construct. In the future, these light boxes could be utilized by other teams to carefully regulate the intensity and duration of light pulses for other light-regulated synthetic biology constructs, in addition to reducing ambient light. To this end, we have created a guide to using these light boxes for CRISPR-Cas9 prokaryotic systems like our own, alongside a putative design for mammalian systems. Please see our guide page for more details.

General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto