Difference between revisions of "Team:Munich/Hardware"

 
(192 intermediate revisions by 5 users not shown)
Line 9: Line 9:
 
<link rel="stylesheet" type="text/css"  
 
<link rel="stylesheet" type="text/css"  
 
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 +
<link rel="stylesheet" type="text/css"
 +
href="https://2017.igem.org/Template:Munich/Filter?action=raw&ctype=text/css" />
 +
<link rel="stylesheet" type="text/css"
 +
href="https://2017.igem.org/Template:Munich/Body?action=raw&ctype=text/css" />
 
<head>
 
<head>
 
<style>
 
<style>
 +
#HQ_page h3{
 +
text-align: left;
 +
margin-bottom: 10px;
 +
}
  
−
</style>
+
#HQ_page h4{
−
</head><body>
+
text-align: left;
−
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
+
}
  
−
<!-- Bar -->
+
#HQ_page h3{
−
<tr><td height="32" bgcolor=#51A7f9></td><td class="no-padding" bgcolor=#51A7f9 height="32" width="960" align=center>
+
color: #51a7f9;
−
<div class="dropdown">
+
}
−
  <a href="/Team:Munich"><button class="dropbtnCascAid dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-content dropdown-content">
+
−
  </div>
+
−
</div><div class="dropdown">
+
−
  <a href="project" target=content><button class="dropbtnProject dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-contentDescription dropdown-content">
+
−
    <a href="description" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentDesign dropdown-content">
+
−
    <a href="design" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentProofofConcept dropdown-content">
+
−
    <a href="proofofconcept" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentDemonstration dropdown-content">
+
−
    <a href="demonstration" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentApplication dropdown-content">
+
−
    <a href="application" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentFinalResults dropdown-content">
+
−
    <a href="finalresults" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentEntrepreneurship dropdown-content">
+
−
    <a href="entrepreneurship" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentAchievements dropdown-content">
+
−
    <a href="achievements" target=content></a>
+
−
  </div>
+
−
</div><div class="dropdown" valign=center>
+
−
  <a href="wetlab" target=content><button class="dropbtnWetlab dropbtn" valign=center><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-contentProtocolsMethods dropdown-content">
+
−
    <a href="protocolsmethods" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentMaterials dropdown-content">
+
−
    <a href="materials" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentLabJournal dropdown-content">
+
−
    <a href="labjournal" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentSafety dropdown-content">
+
−
    <a href="safety" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentParts dropdown-content">
+
−
    <a href="parts" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentInterlab dropdown-content">
+
−
    <a href="interlab" target=content></a>
+
−
  </div>
+
−
</div><div class="dropdown">
+
−
  <a href="model" target=content><button class="dropbtnModel dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-content dropdown-content">
+
−
  </div>
+
−
</div><div class="dropdown">
+
−
  <a href="softhardware" target=content><button class="dropbtnSoftHardware dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-contentSoftware dropdown-content">
+
−
    <a href="software" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentHardware dropdown-content">
+
−
    <a href="hardware" target=content></a>
+
−
  </div>
+
−
</div><div class="dropdown">
+
−
  <a href="hp" target=content><button class="dropbtnHP dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-contentHPSilver dropdown-content">
+
−
    <a href="hpsilver" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentIHPgold dropdown-content">
+
−
    <a href="ihpgold" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentPublicEngagement dropdown-content">
+
−
    <a href="publicengagement" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentCollaborations dropdown-content">
+
−
    <a href="collaborations" target=content></a>
+
−
  </div>
+
−
</div><div class="dropdown">
+
−
  <a href="/Team:Munich/Team" target=content><button class="dropbtnTeam dropbtn"><img src="overlay.png"></button></a>
+
−
  <div class="dropdown-contentTeamMembers dropdown-content">
+
−
    <a href="teammembers" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentSupervisors dropdown-content">
+
−
    <a href="supervisors" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentPIs dropdown-content">
+
−
    <a href="pis" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentCollaboratingInstitutions dropdown-content">
+
−
    <a href="collaboratinginstitutions" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentSponsors dropdown-content">
+
−
    <a href="sponsors" target=content></a>
+
−
  </div>
+
−
  <div class="dropdown-contentAttributions dropdown-content">
+
−
    <a href="/Team:Munich/Team" target=content></a>
+
−
  </div>
+
−
</div>
+
−
</td><td height="32" bgcolor=#51A7f9></td></tr>
+
−
<!-- Content -->
+
−
<tr><td width="100%" colspan=3>
+
−
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
+
−
<tr>
+
−
<td width="40%">
+
−
</td>
+
−
<td id="myContent" width="20%" valign=top align=center>
+
−
<br>
+
−
<!-- Head End -->
+
−
<!-- Content Begin -->
+
−
<img width="960" src="https://static.igem.org/mediawiki/2017/a/ab/T--Munich--team-picture.png" style="margin-bottom: 15px">
+
−
<table width="900" border=0 cellspacing=0 cellpadding=10>
+
−
<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
+
−
<br><br><br><center><hr></center>
+
−
</td></tr>
+
−
</table>
+
−
<!-- Content End -->
+
  
  
 +
#myContent *{
 +
color: #444444;
 +
}
  
 +
#myContent tr p{
 +
margin-bottom: 10px;
 +
}
  
  
 +
#HQ_page .equationDiv img{
 +
height: 40px;
 +
margin-bottom: 10px;
 +
}
  
 +
#HQ_page .equationDiv img.largeEquation{
 +
height: 80px;
 +
}
  
 +
#HQ_page .equationDiv span{
 +
float: right;
 +
}
  
 +
#HQ_page .myTable .rightAligned{
 +
text-align: right;
 +
}
 +
#HQ_page .myTable .leftAligned{
 +
text-align: left;
 +
}
  
 +
#HQ_page .myTable th{
 +
  color: #919191;
 +
}
  
 +
#HQ_page #hardwareFrontPage td{
 +
padding:0;
 +
}
  
 +
#HQ_page #hardwareFrontPage img{
 +
background-size: 100% 100%;
 +
}
  
 +
#HQ_page #hardwareFrontPage img:hover{
 +
background-size: 100% 100%;
 +
}
  
 +
#HQ_page .picture1{
 +
width: 320px;
 +
height: 155px;
 +
background: url("https://static.igem.org/mediawiki/2017/4/45/Valve_hw_home.jpeg")
 +
}
  
−
<!-- Foot Begin -->
+
#HQ_page .picture1:hover{
−
<table id="myFooter" width=960 height=35 cellpadding=0 cellspacing=0 border=0><tr><td align=right class="no-padding" valign=center>
+
background: url("https://static.igem.org/mediawiki/2017/5/53/Pressure_supply_hw_home2.png")
 +
}
  
−
<table width=300 height=35 cellpadding=0 cellspacing=0 border=0><tr>
+
#HQ_page #picture2{
 +
width: 320px;
 +
height: 210px;
 +
background: url("https://static.igem.org/mediawiki/2017/e/e1/Paper_strip_hw_home.jpeg")
 +
}
  
−
<td align=center valign=center>
+
#HQ_page #picture2:hover{
−
<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
+
background: url("https://static.igem.org/mediawiki/2017/2/22/Paper_strip_hw_home3.png")
−
<img src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_coop_lmu_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg'">
+
}
−
</a>
+
−
</td>
+
  
−
<td align=center valign=center>
+
#HQ_page #picture3{
−
<a href="https://www.tum.de/" target="_blank4" >
+
width: 320px;
−
<img src="https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/1/1e/T--Munich--wiki_image_coop_tum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg'">
+
height: 210px;
−
</a>
+
background: url("https://static.igem.org/mediawiki/2017/3/39/Heater_hw_home.jpeg")
−
</td>
+
}
  
−
<td align=center valign=center>
+
#HQ_page #picture3:hover{
−
<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
+
background: url("https://static.igem.org/mediawiki/2017/5/5d/Heater_cad_hw_home.png")
−
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/72/T--Munich--wiki_image_coop_e14_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg'">
+
}
−
</a>
+
−
</td>
+
  
−
<td align=center valign=center>
+
#HQ_page #picture4{
−
<a href="http://www.biochem.mpg.de/en" target="_blank5" >
+
width: 640px;
−
<img src="https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg'">
+
height: 555px;
−
</a>
+
background: url("https://static.igem.org/mediawiki/2017/7/7d/Detector_hw_startpage.jpeg");
−
</td>
+
}
  
−
<td align=center valign=center>
+
#HQ_page #picture4:hover{
−
<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
+
background: url("https://static.igem.org/mediawiki/2017/2/2e/Detector_hw_home.png")
−
<img src="https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6b/T--Munich--wiki_image_coop_helmholtzzentrum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg'">
+
}
−
</a>
+
−
</td>
+
  
−
<td align=center valign=center>
+
</style>
−
<a href="https://igem.org/Main_Page" target="_blank6" >
+
</head></html>
−
<img src="https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_coop_igem_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg'">
+
{{Munich/Menu}}
−
</a>
+
<html>
 +
<body>
 +
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
 +
<!-- Content -->
 +
<tr><td width="100%" colspan=4>
 +
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
 +
<tr>
 +
<td width="40%">
 
</td>
 
</td>
 +
<td id="myContent" width="20%" valign=top align=center>
 +
<br>
 +
<!-- Head End -->
 +
<!-- Content Begin -->
 +
<table width="960" border=0 cellspacing=0 cellpadding=10>
 +
<tr>
 +
<td width=160></td>
 +
<td width=160></td>
 +
<td width=160></td>
 +
<td width=160></td>
 +
<td width=160></td>
 +
<td width=160></td>
 +
</tr>
 +
<tr id="hardwareFrontPage">
 +
<td colspan=2>
 +
<table width=320>
  
−
</tr></table></td><td class="no-padding" align=center valign=center><table width=35 height=35 cellpadding=0 cellspacing=0 border=0><tr><td width="35" align=center valign=center></td></tr></table></td><td class="no-padding" align=left valign=center><table width=200 height=35 cellpadding=0 cellspacing=0 border=0><tr>
+
<tr>
−
 
+
<td>
−
<td align=center valign=center>
+
<a href="/Team:Munich/Hardware/QuakeValve"><img class="picture1" src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></a>
−
<a href="https://www.facebook.com/Munich.iGEM" target="_blank7" >
+
−
<img src="https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/4/44/T--Munich--wiki_image_sm_facebook_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg'">
+
−
</a>
+
 
</td>
 
</td>
−
 
+
<td>
−
<td align=center valign=center>
+
−
<a href="https://twitter.com/iGEM_Munich" target="_blank8" >
+
−
<img src="https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/c/cc/T--Munich--wiki_image_sm_twitter_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg'">
+
−
</a>
+
−
</td>
+
−
 
+
−
<td align=center valign=center>
+
−
<a href="mailto:igem.munich@gmail.com">
+
−
<img src="https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/9/93/T--Munich--wiki_image_sm_mail_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg'">
+
−
</a>
+
−
</td>
+
−
 
+
−
<td align=center valign=center>
+
−
<a href="https://www.instagram.com/igem.munich.2017/" target="_blank9" >
+
−
<img src="https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6e/T--Munich--wiki_image_sm_instagram_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg'">
+
−
</a>
+
−
</td>
+
−
 
+
−
<td align=center valign=center>
+
−
<a href="https://2017.igem.org/Team:Munich" target="_blank10" >
+
−
<img src="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--wiki_image_sm_web_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/61/T--Munich--wiki_image_sm_web_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/1/1d/T--Munich--wiki_image_sm_web_low.svg'">
+
−
</a>
+
−
</td>
+
−
 
+
−
</tr></table>
+
−
</td></table>
+
−
</td>
+
−
<td width="40%">
+
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
−
</body>
 
−
</td></tr>
 
−
<tr><td height="25" bgcolor=#ffffff></td><td class="no-padding" bgcolor=#ffffff height="25" width="960" align=center>
 
−
<hr>
 
−
<table height=25 cellpadding=0 cellspacing=2 border=0><tr>
 
  
−
<td bgcolor=#ffffff height="25" width="90" align=center valign=center>
+
<a href="/Team:Munich/Hardware/SampleProcessing"><img id="picture3" src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></a>
−
<a href="https://www.eurofinsgenomics.eu/" target="_blank1" ><img src="https://static.igem.org/mediawiki/2017/c/c7/T--Munich--wiki_image_sponsors_eurofins.svg" border=0 height="15"></a>
+
<a href="/Team:Munich/Hardware/Paperstrip"><img id="picture2" src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></a>
 
</td>
 
</td>
−
 
+
<td colspan=4>
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
<a href="/Team:Munich/Hardware/Detector"><img id="picture4" src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></a>
−
 
+
−
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
−
<a href="https://www.neb.com/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/8/87/T--Munich--wiki_image_sponsors_neb.svg" border=0 height="21"></a>
+
 
</td>
 
</td>
 +
</tr>
  
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
<tr>
 +
<td colspan=6>
 +
<div class="captionPicture">
 +
<p>
 +
Complete overview of all modular hardware parts in our pathogen detection system. Shown are counterclockwise and starting in in the upper left corner: The Quake valve that controls fluid flow, our sample processing device, the paper strip where a reaction mix is stored and the readout reaction takes place and finally our low-cost fluorescence detector "Lightbringer" that performs the readout measurement. Images are clickable and linked to the corresponding wiki subsection.
  
−
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
</p>
−
<a href="https://www.biomers.net" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/a/a9/T--Munich--wiki_image_sponsors_biomers.png" border=0 height="21"></a>
+
</div></td>
−
</td>
+
</tr>
  
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
<tr><td colspan=6 align=left valign=center>
−
 
+
<font size=7 color=#51a7f9><b style="color: #51a7f9">Hardware</b></font>
−
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
−
<a href="https://www.gatc-biotech.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/5/59/T--Munich--wiki_image_sponsors_gatc.png" border=0 height="21"></a>
+
 
</td>
 
</td>
 +
</tr>
 +
<tr>
 +
<td  colspan = 6 align=center valign=center>
 +
<p class="introduction">
 +
 +
The liberation of diagnostic tests from expensive lab infrastructure requires innovative ways of sample processing and measuring. We therefore developed a set of portable hardware tools with the goal of providing an automated sample-to-answer solution. The heart of our system is <a class="myLink" href="https://2017.igem.org/Team:Munich/Hardware/Detector">‘Lightbringer’</a>, our fluorescence detector, which is capable of measuring kinetics of biological or chemical reactions on <a class="myLink" href= "https://2017.igem.org/Team:Munich/Hardware/Paperstrip" >paper.</a> Built from 3D–printed parts and standard electronic components, it can be assembled for less than 15$, while offering a sensitivity competitive to commercial fluorescence readers. Additionally, tackling the challenge of sample pre-processing in field, we developed a portable <a class="myLink" href="https://2017.igem.org/Team:Munich/Hardware/SampleProcessing">  fluidic system</a>, featuring a temperature control unit for lysis and isothermal PCR. Conceiving a platform independent of lab infrastructure, we demonstrate the feasibility of <a class="myLink" href="https://2017.igem.org/Team:Munich/Hardware/QuakeValve"> controlling fluid flow</a> with bike tires and air balloons. All hardware components are designed and documented with the aim of enabling the community to reproduce and extend our set of tools.
 +
                </p>
  
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
<div class="captionPicture">
 +
<img width=960 src="https://static.igem.org/mediawiki/2017/2/26/Schema_final_lowres.png">
 +
<p>
 +
</p>
 +
</div>
  
−
<td bgcolor=#ffffff height="25" width="70" align=center valign=center>
 
−
<a href="https://www3.gehealthcare.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/1/1b/T--Munich--wiki_image_sponsors_gehealthcare.svg" border=0 height="21"></a>
 
 
</td>
 
</td>
 +
</tr>
  
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
 
  
−
<td bgcolor=#ffffff height="25" width="85" align=center valign=center>
 
−
<a href="https://eu.idtdna.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/1/1a/T--Munich--wiki_image_sponsors_idt.png" border=0 height="21"></a>
 
−
</td>
 
  
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
<tr><td class="no-padding" colspan=6 align=right valign=center height=10>
−
 
+
<br><br><br><center><hr></center>
−
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
</td></tr>
−
<a href="https://www.nano-initiative-munich.de/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/a/a7/T--Munich--wiki_image_sponsors_nim.png" border=0 height="21"></a>
+
</table>
−
</td>
+
<!-- Content End -->
−
 
+
</html>
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
{{Munich/Footer}}
−
 
+
−
<td bgcolor=#ffffff height="25" width="120" align=center valign=center>
+
−
<a href="https://www.promega.de/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--wiki_image_sponsors_promega.png" border=0 height="21"></a>
+
−
</td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="40" align=center valign=center>
+
−
<a href="https://www.qiagen.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/9/99/T--Munich--wiki_image_sponsors_qiagen.svg" border=0 height="21"></a>
+
−
</td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="40" align=center valign=center>
+
−
<a href="https://www.carlroth.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/d/d6/T--Munich--wiki_image_sponsors_roth.png" border=0 height="21"></a>
+
−
</td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="130" align=center valign=center>
+
−
<a href="https://www.scienova.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/3/3b/T--Munich--wiki_image_sponsors_scienova.png" border=0 height="21"></a>
+
−
</td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
−
 
+
−
<td bgcolor=#ffffff height="25" width="120" align=center valign=center>
+
−
<a href="https://www.unternehmertum.de" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/b/b2/T--Munich--wiki_image_sponsors_unternehmertum.png" border=0 height="21"></a>
+
−
</td>
+
−
 
+
−
</tr></table>
+
−
 
+
−
 
+
−
</td><td height="25" bgcolor=#ffffff></td></tr>
+
−
</table></body></html>
+

Latest revision as of 09:14, 9 December 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Complete overview of all modular hardware parts in our pathogen detection system. Shown are counterclockwise and starting in in the upper left corner: The Quake valve that controls fluid flow, our sample processing device, the paper strip where a reaction mix is stored and the readout reaction takes place and finally our low-cost fluorescence detector "Lightbringer" that performs the readout measurement. Images are clickable and linked to the corresponding wiki subsection.

Hardware

The liberation of diagnostic tests from expensive lab infrastructure requires innovative ways of sample processing and measuring. We therefore developed a set of portable hardware tools with the goal of providing an automated sample-to-answer solution. The heart of our system is ‘Lightbringer’, our fluorescence detector, which is capable of measuring kinetics of biological or chemical reactions on paper. Built from 3D–printed parts and standard electronic components, it can be assembled for less than 15$, while offering a sensitivity competitive to commercial fluorescence readers. Additionally, tackling the challenge of sample pre-processing in field, we developed a portable fluidic system, featuring a temperature control unit for lysis and isothermal PCR. Conceiving a platform independent of lab infrastructure, we demonstrate the feasibility of controlling fluid flow with bike tires and air balloons. All hardware components are designed and documented with the aim of enabling the community to reproduce and extend our set of tools.