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| − | <li>We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in <i>S. cerevisiae</i>.</li>
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| − | <li>By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents </li>
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| − | <li>With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.</li>
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| − | <li>Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.</li>
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| − | <li>By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox</li>
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| − | <li>We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.</li>
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| − | <li>With a high throughput assay we characterized the import efficiency of different PTS2 sequences.</li>
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| − | <li>To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.</li>
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| − | <li>For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.</li>
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