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The system that was already introduced in the project description looks a lot like in the figure below. The Scaffold Construct represents the three functional 14-3-3 monomers. We also described that the Scaffold construct would be a tetramer, but because we mutated the last pocket, the pocket has no function and could be left out of the model. At each functional pockets, an inducer can bind and facilitates the binding of the Binding Partner.<br/> | The system that was already introduced in the project description looks a lot like in the figure below. The Scaffold Construct represents the three functional 14-3-3 monomers. We also described that the Scaffold construct would be a tetramer, but because we mutated the last pocket, the pocket has no function and could be left out of the model. At each functional pockets, an inducer can bind and facilitates the binding of the Binding Partner.<br/> | ||
− | The Binding Partner represents the CT33 constructs, with include a | + | The Binding Partner represents the CT33 constructs, with include a Strep-tag®II. The CT33 site can bind in the pockets of the Scaffold construct filled with inducer and the Strep-tag®II site can bind to Strep-Tactin®XT, represented as the Center Point. The center point has four sites, which can individually bind to the Strep-tag®II and cause a valency of four.</h6> |
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Latest revision as of 13:48, 10 December 2017