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| <img src="https://static.igem.org/mediawiki/2017/5/5a/T--IISER-Mohali-INDIA--Co-culture.png" width="100%" height="100%"></div> | | <img src="https://static.igem.org/mediawiki/2017/5/5a/T--IISER-Mohali-INDIA--Co-culture.png" width="100%" height="100%"></div> |
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− | <div class="page-title"><h5>Co-culture and Growth</h5> | + | <div class="page-title">Co-culture and Growth |
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| <section> | | <section> |
− | <h3>Here in we have the plan to co-culture our engineered strain of <i>E.coli</i> (which can independently detect and capture each of the noxious gases and harmful chemicals) with naturally occurring microbes which can consume these harmful gases and chemicals through their evolved metabolic pathways. This ensures the existence of a sink for these pollutants, and thereby the longevity of the synthetic microbes. </h3> | + | <h3>Here in we have the plan to co-culture our engineered strain of <i>E. coli</i> (which can independently detect and capture each of the noxious gases and harmful chemicals) with naturally occurring microbes which can consume these harmful gases and chemicals through their evolved metabolic pathways. This ensures the existence of a sink for these pollutants, and thereby the longevity of the synthetic microbes. </h3> |
| </section> | | </section> |
| <section><h3>Simply introducing the metabolic pathways of qualified co-culture cells (which can consume these harmful gases and chemicals) in a synthetic microbe induces immense burden and reduces its longevity. This approach has been taken up by many teams, only to result in the non-viability of these microbes. Therefore, we have taken a novel co-culturing method which is partially synthetic and partially natural to tackle pollution in a more efficient and effective manner.</h3></section> | | <section><h3>Simply introducing the metabolic pathways of qualified co-culture cells (which can consume these harmful gases and chemicals) in a synthetic microbe induces immense burden and reduces its longevity. This approach has been taken up by many teams, only to result in the non-viability of these microbes. Therefore, we have taken a novel co-culturing method which is partially synthetic and partially natural to tackle pollution in a more efficient and effective manner.</h3></section> |
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| <tr> | | <tr> |
| <td class="text-left">Nitric Oxide</td> | | <td class="text-left">Nitric Oxide</td> |
− | <td class="text-left">species of <i>Pseudomonas, Alkaligenes , Bacillus and algae -Dunaliella tertiolecta</i></td> | + | <td class="text-left">species of <i>Pseudomonas, Alkaligenes,</i> Bacillus and algae - <i>Dunaliella tertiolecta</i></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td class="text-left">Lead</td> | | <td class="text-left">Lead</td> |
− | <td class="text-left"><i>Clostridium formicoaceticum ,C.thermoaceticum, | + | <td class="text-left"><i>Clostridium formicoaceticum ,C.thermoaceticum,</i> |
− | algae -Chlorella vutgaris, Spirogyra</i></td> | + | algae - <i>Chlorella vutgaris, Spirogyra</i></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td class="text-left">Uranium</td> | | <td class="text-left">Uranium</td> |
− | <td class="text-left">algae -<i>Cladophora hutchinsiae</i></td> | + | <td class="text-left">algae - <i>Cladophora hutchinsiae</i></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td class="text-left">Chromium</td> | | <td class="text-left">Chromium</td> |
− | <td class="text-left">algae -<i>Oedogonium hatei</i></td> | + | <td class="text-left">algae - <i>Oedogonium hatei</i></td> |
| </tr> | | </tr> |
| <td class="text-left">Copper</td> | | <td class="text-left">Copper</td> |
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| </tr> | | </tr> |
| <td class="text-left">Zinc</td> | | <td class="text-left">Zinc</td> |
− | <td class="text-left"><i>algae -Gelidium</i></td> | + | <td class="text-left">algae - <i>Gelidium</i></td> |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
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| </center> | | </center> |
| <br/><br/> | | <br/><br/> |
− | <div class="img-data"><img src="https://static.igem.org/mediawiki/2017/b/bd/T--IISER-Mohali-INDIA--hrdwrppr.png"></div> | + | <center><div class="img-data"><img src="https://static.igem.org/mediawiki/2017/b/bd/T--IISER-Mohali-INDIA--hrdwrppr.png" height="30%" width="30%"></div></center> |
− | <section><div class="page-title"><h6>Culturing on paper</h6></div> | + | <section><div class="page-title">Culturing on paper</div><br/><br/> |
| <h3>The study of bacterial growth has been one of the most standard approaches in the field of microbiology | | <h3>The study of bacterial growth has been one of the most standard approaches in the field of microbiology |
− | and Escherichia coli as a workhorse has been considerably exploited for its use in many molecular | + | and <i>Escherichia coli</i> as a workhorse has been considerably exploited for its use in many molecular |
| biology techniques. The need of a nutrient rich solid or liquid medium is of utmost importance for the | | biology techniques. The need of a nutrient rich solid or liquid medium is of utmost importance for the |
| sustenance of bacterial growth. However, people over the past few years have tried using paper based | | sustenance of bacterial growth. However, people over the past few years have tried using paper based |
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| </section> | | </section> |
| <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/0/0b/T--IISER-Mohali-INDIA--hardware1.png" width="100%"></td></tr></table></center> | | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/0/0b/T--IISER-Mohali-INDIA--hardware1.png" width="100%"></td></tr></table></center> |
− | | + | <h5><center>Arrangement of cultured E. coli colonies after printing on a sheet containing agar [1]</center></h5> |
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| dispense bacteria. Am. J. Biochem. Biotechnol, 10, 81-87. | | dispense bacteria. Am. J. Biochem. Biotechnol, 10, 81-87. |
| </h4><h4> | | </h4><h4> |
− | Schmidt, A., Frensch, M., Schleheck, D., Schink, B., & Müller, N. (2014).Degradation of Acetaldehyde and Its Precursors by <i>Pelobactercarbinolicus</i> and <i>P. acetylenicus. PloS one</i>, 9(12), e115902.</h4> | + | 2. Schmidt, A., Frensch, M., Schleheck, D., Schink, B., & Müller, N. (2014).Degradation of Acetaldehyde and Its Precursors by <i>Pelobactercarbinolicus</i> and <i>P. acetylenicus. PloS one</i>, 9(12), e115902.</h4> |
| <h4> | | <h4> |
− | Jin, Y., Veiga, M. C., &Kennes, C. (2005).Bioprocesses for the removal of nitrogen oxides from polluted air. <i>Journal of Chemical Technology and Biotechnology</i>, 80(5), 483-494.</h4> | + | 3. Jin, Y., Veiga, M. C., &Kennes, C. (2005).Bioprocesses for the removal of nitrogen oxides from polluted air. <i>Journal of Chemical Technology and Biotechnology</i>, 80(5), 483-494.</h4> |
− | <h4>François, F., Lombard, C., Guigner, J. M., Soreau, P., Brian-Jaisson, F., Martino, G., ...&Peduzzi, J. (2012). Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury. <i>Applied and environmental microbiology</i>, 78(4), 1097-1106.</h4> | + | <h4>4. François, F., Lombard, C., Guigner, J. M., Soreau, P., Brian-Jaisson, F., Martino, G., ...&Peduzzi, J. (2012). Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury. <i>Applied and environmental microbiology</i>, 78(4), 1097-1106.</h4> |
− | <h4>Wang, X., Wang, Q., Li, S., & Li, W. (2015). Degradation pathway and kinetic analysis for p-xylene removal by a novel <i>Pandoraea sp.</i> strain WL1 and its application in a biotrickling filter. <i>Journal of hazardous materials</i>, 288, 17-24. | + | <h4>5. Wang, X., Wang, Q., Li, S., & Li, W. (2015). Degradation pathway and kinetic analysis for p-xylene removal by a novel <i>Pandoraea sp.</i> strain WL1 and its application in a biotrickling filter. <i>Journal of hazardous materials</i>, 288, 17-24. |
| </h4> | | </h4> |
− | <h4>Diekert, G. B., &Thauer, R. K. (1978).Carbon monoxide oxidation by <i>Clostridium thermoaceticum</i> and <i>Clostridium formicoaceticum. Journal of bacteriology</i>, 136(2), 597-606.</h4> | + | <h4>6. Diekert, G. B., &Thauer, R. K. (1978).Carbon monoxide oxidation by <i>Clostridium thermoaceticum</i> and <i>Clostridium formicoaceticum. Journal of bacteriology</i>, 136(2), 597-606.</h4> |
| | | |
− | <h4>Chappelle, E. W. (1962). Carbon monoxide oxidation by algae. <i>Biochimicaetbiophysicaacta</i>, 62(1), 45-62. | + | <h4>7. Chappelle, E. W. (1962). Carbon monoxide oxidation by algae. <i>Biochimicaetbiophysicaacta</i>, 62(1), 45-62. |
| Slomczynski, D. J. AND W J. DavisHoover*.CHARACTERIZATION OF PB2+ UPTAKE AND SEQUESTRATION IN <i>PSEUDOMONAS AERUGINOSA</i>, CHL004, LEAD.Presented at Third International Conference, Monterey, CA, 5/20-23/2002.</h4> | | Slomczynski, D. J. AND W J. DavisHoover*.CHARACTERIZATION OF PB2+ UPTAKE AND SEQUESTRATION IN <i>PSEUDOMONAS AERUGINOSA</i>, CHL004, LEAD.Presented at Third International Conference, Monterey, CA, 5/20-23/2002.</h4> |
− | <h4>Wolfe-Simon, F., Blum, J. S., Kulp, T. R., Gordon, G. W., Hoeft, S. E., Pett-Ridge, J., ...& Anbar, A. D. (2011). A bacterium that can grow by using arsenic instead of phosphorus. <i>science</i>, 332(6034), 1163-1166. | + | <h4>8. Wolfe-Simon, F., Blum, J. S., Kulp, T. R., Gordon, G. W., Hoeft, S. E., Pett-Ridge, J., ...& Anbar, A. D. (2011). A bacterium that can grow by using arsenic instead of phosphorus. <i>science</i>, 332(6034), 1163-1166. |
| </h4></section><br/><br/> | | </h4></section><br/><br/> |
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