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| − | {{Heidelberg/title|Human Practices Overview}}
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| − | <div class="header-title heidelberg-red"> Human Practices </div>
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| − | <div class="header-subtitle" style="color: #393939 !important;"> Overview </div>
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| − | Successful in vivo directed evolution by PREDCEL and PACE requires the thorough consideration of experimental parameters, e.g. phage propagation times, culture dilution rates and inducer/inhibitor concentrations. We employed extensive ODE-based and stochastic modeling to identify the most sensitive parameters and adapt our experiments accordingly. First, we calibrated our models using phage propagation experiments from our wet lab complemented with literature data. Simulations showed that the phage titer is highly sensitive to culture dilution rates. We simulated batch times and transfer volumes for PREDCEL and corresponding flow rates for PACE to determine optimized conditions for gene pool selection while avoiding phage washout. We also estimated phage titer monitoring intervals for cost/labor efficient QC as well as inducer/inhibitor concentrations required to express the required mutagenic polymerases. Finally, provide a web-based, fully interactive modeling platform, not only extensively employed by our wet lab, but highly informs future iGEM teams building on our work.
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| − | {{Heidelberg/templateus/Heading|Human Practices - Gold Medal Criteria}}
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| − | {{Heidelberg/overviewpanel2|ffdb4d|
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| − | {{Heidelberg/panelelement2|ffdb4d|Engineering Cycle|https://static.igem.org/mediawiki/2017/9/90/T--Heidelberg--2017_Police_responsibility.png|https://2017.igem.org/Team:Heidelberg/HP/Gold_Integrated#1|
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| − | Responsible science requires responsible scientists to respond to responsible responses responsibly.
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| − | |Extended Engineering Cycle
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| − | {{Heidelberg/panelelement2|ffdb4d|SafetyNet|https://static.igem.org/mediawiki/2017/1/17/T--Heidelberg--2017_Connections_black.png|https://2017.igem.org/Team:Heidelberg/HP/Gold_Integrated#2|
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| − | Colololololololololo lolololo lolo lo lol ololololo lolololololololololo lolololol olololo lolololololololo loloololo lolaborations.
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| − | |More on our SafetyNet
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| − | {{Heidelberg/panelelement2|ffdb4d|Ecological & Medical Relevance|https://static.igem.org/mediawiki/2017/c/cd/T--Heidelberg--2017_Lab_Equip_black.png|https://2017.igem.org/Team:Heidelberg/HP/Gold_Integrated#3|
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| − | Many experienced experts in their respective expert fields were expertifically interviewed during our Expert Interviews.
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| − | |Relevance
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