Difference between revisions of "Team:Toronto/Parts"

 
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<h1>Parts</h1>
 
<h1>Parts</h1>
 
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<h2>Submitted Parts</h2>
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<figure>
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<table>
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<td>pSB1C3</td>
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      <td>Registry number </td>
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      <td>Type</td>
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      <td>Length (bp)</td>
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      <td>Function </td>
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      <td>Assembly Method</td>
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      <td>UNS</td>
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<td>Hello</td>
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<td>2 ads fads fdsa s </td>
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<td>1</td>
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<td>911 asd afd </td>
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<figcaption>Figure 1.1: Wildlife population is wildlife population is wildlife population is wildlife population</figcaption>
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<h2 class="text-red">Submitted Parts</h2>
  
 
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      <th>pSB1C3</th>
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      <th>Registry number </th>
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      <th>Type</th>
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      <th>Length (bp)</th>
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      <th>Function </th>
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      <th>Assembly Method</th>
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      <th>UNS</th>
 
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  <tr>
 
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      <td>LacILOV-mCherry </td>
 
      <td>LacILOV-mCherry </td>
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      <td>BBa_K2469003</td>
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      <td><a style="text-decoration: underline !important" href="http://parts.igem.org/Part:BBa_K2469003">BBa_K2469003</a></td>
 
      <td>Composite </td>
 
      <td>Composite </td>
 
      <td>1615</td>
 
      <td>1615</td>
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  <tr>
 
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      <td>HIS-AcrIIA4</td>
 
      <td>HIS-AcrIIA4</td>
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      <td>BBa_K2469002</td>
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      <td><a style="text-decoration: underline !important" href="http://parts.igem.org/Part:BBa_K2469002">BBa_K2469002</a></td>
 
      <td>Basic</td>
 
      <td>Basic</td>
 
      <td>398</td>
 
      <td>398</td>
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  <tr>
 
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      <td>LacILOV</td>
 
      <td>LacILOV</td>
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      <td>BBa_K2469001</td>
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      <td><a style="text-decoration: underline !important" href="http://parts.igem.org/Part:BBa_K2469001">BBa_K2469001</a></td>
 
      <td>Basic</td>
 
      <td>Basic</td>
 
      <td>728</td>
 
      <td>728</td>
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      <td>A fusion protein of the light, oxygen and voltage-sensitive domain of the Avena Sativa phototropin 1 (LOV2) and the LacI domain. This transcriptional regulator represses promoters under control of LacI. In the presence of blue light this protein will act as a repressor, and in its absence the protein will dissociate from the promoter </td>
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      <td>A fusion protein of the light, oxygen and voltage-sensitive domain of the Avena Sativa phototropin 1 (LOV2) and the LacI domain. This transcriptional regulator represses promoters under control of LacI. It is speculated that in the presence of blue light, LacILOV dissociates, causing it to unbind from DNA and allow transcription. In the absence of light, the protein dimerizes and binds to the promoter of lac operon, inhibiting the transcription of downstream genes.</td>
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      <td>Gibson assembly of amplified LacILOV part from LacILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
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<td>Gibson assembly of amplified LacILOV part from LacILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
 
      <td>2, 3</td>
 
      <td>2, 3</td>
 
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      <td>reporter construct </td>
 
      <td>reporter construct </td>
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      <td>BBa_K2469004</td>
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      <td><a style="text-decoration: underline !important" href="http://parts.igem.org/Part:BBa_K2469004">BBa_K2469004</a></td>
 
      <td>Composite </td>
 
      <td>Composite </td>
 
      <td>3253</td>
 
      <td>3253</td>
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<figcaption>Figure 1.1: Parts</figcaption>
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<figcaption>Table 1: List of parts that was submitted to iGEM registry.</figcaption>
 
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      <th>Length</th>
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<figcaption>Figure 1.1: Other parts</figcaption>
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<figcaption>Table 2: Other parts used in our project that was not submitted to iGEM registry.</figcaption>
 
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<h2 class="text-cyan">References</h2>
 
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<li id="ref1">Canadian Biosafety Standards and Guidelines - <a href="https://www.canada.ca/en/public-health/services/canadian-biosafety-standards-guidelines.html">https://www.canada.ca/en/public-health/services/canadian-biosafety-standards-guidelines.html</a></li>
 
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<li id="ref2">Biosafety Team in the Office of Environmental Health and Safety - <a href="https://ehs.utoronto.ca/our-services/biosafety/">https://ehs.utoronto.ca/our-services/biosafety/</a></li>
 
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<li id="ref3">NIH Guidelines - <a href="https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment.html">https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment.html</a></li>
 
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<li id="ref4">iGEM Risk Groups - <a href="https://2017.igem.org/Safety/Risk_Groups">https://2017.igem.org/Safety/Risk_Groups</a></li>
 
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Latest revision as of 19:53, 14 December 2017

Parts

Submitted Parts

pSB1C3 Registry number Type Length (bp) Function Assembly Method UNS
LacILOV-mCherry BBa_K2469003 Composite 1615 Light regulated reporter Digestion and ligation 2, 3
HIS-AcrIIA4 BBa_K2469002 Basic 398 Inhibits the function of S. pyogenes Cas9 and dCas9. Gibson assembly of amplified AcrIIA4 from G-block and amplified pSB1C3 backbone from LacILOV-mCherry Construct in pSB1C3 2, 3
LacILOV BBa_K2469001 Basic 728 A fusion protein of the light, oxygen and voltage-sensitive domain of the Avena Sativa phototropin 1 (LOV2) and the LacI domain. This transcriptional regulator represses promoters under control of LacI. It is speculated that in the presence of blue light, LacILOV dissociates, causing it to unbind from DNA and allow transcription. In the absence of light, the protein dimerizes and binds to the promoter of lac operon, inhibiting the transcription of downstream genes. Gibson assembly of amplified LacILOV part from LacILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3 2, 3
reporter construct BBa_K2469004 Composite 3253 Light regulated reporter switch, alternating between mCherry and YFP flourescence Gibson assembly of amplified reporter construct out of pkDL071 and amplified pSB1C3 backbone from the LacILOV-mCherry construct in pSB1C3 2, 3
Table 1: List of parts that was submitted to iGEM registry.

Other Parts

pKDLO71 Type Length Function Assembly Method UNS
LacILOV-mCherry Composite 1615 Light regulated reporter Gibson g-Block + Backbone with UNS 2, 3
reporter mCherry reporter construct, consisting of the CI repressor (with LVA degradation tag) and mCherry under the control of the Ptrc-2 promoter; used as a negative control in the sgRNA assay Gibson g-Block + Backbone with UNS 2, 4
reporter mCherry + sgRNA Half of the full construct, to test the function of the sgRNA (ensure it is targetting the correct sequence) Gibson assembly of sgRNA g-Block into reporter mCherry, with UNS 5 extension on backbone 4, 5
reporter mCherry + sgRNA+ AcrIIA4 Incorporation of the Anti-CRISPR protein into the sgRNA construct to have both expressed simultaneously and test the function of the Anti-CRISPR Gibson assemly of AcrIIA4 g-block into reporter mCherry + sgRNA, with UNS 6 extension on backbone 4, 6
reporter construct Composite 3253 Light regulated reporter switch, alternating between mCherry and YFP flourescence Gibson assembly of reporter YFP (LacILOV + YFP) into reporter mCherry with UNS 3 extension on backbone 3, 2
full construct Light-regulated switch controlling the activity of the CRISPR-Cas9 system. Gibson assembly of AcrIIA4 subpart (LacILOV + AcrIIA4 + YFP) into reporter mCherry + sgRNA, with UNS 3 extension on bacbone 3, 6
Table 2: Other parts used in our project that was not submitted to iGEM registry.

General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto