Difference between revisions of "Team:Mingdao"
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| − | According to research | + | According to research, about 7 adults in 10 suffer the problem of overweight or even obesity, and it is expected that more people are going to get into this kind of unhealthy problem. One of the most major factors that lead to obesity is “sugar”, which could be easily found in beverages, dessert, and almost any kind of food. Thus, it is quite difficult not to absorb too much amount of sugar in our daily lives. In hope of preventing our bodies from absorbing too much glucose, our team aims to engineer bacteria that absorb glucose more efficiently. Our team constructed glucose active transporters on the membrane of E.coli, enabling the glucose uptake to become faster than intestine cells. On the other hand, our team has designed a suicide circuit ensuring the bacteria don’t absorb all the sugar and the body can get adequate amount of sugar. |
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<img class="d-block w-100" src="https://static.igem.org/mediawiki/2017/6/6a/Mingdaophil1020-4.png" alt="Fourth slide"> | <img class="d-block w-100" src="https://static.igem.org/mediawiki/2017/6/6a/Mingdaophil1020-4.png" alt="Fourth slide"> | ||
<div class="carousel-caption d-none d-md-block"> | <div class="carousel-caption d-none d-md-block"> | ||
| − | <h3 style="letter-spacing: 0.1rem"> | + | <h3 style="letter-spacing: 0.1rem">Modeling & Acid-Resistant Pill</h3> |
<h4>Lactobacillus was encapsulated into gel sphere which is acid resistant in pH=2 (gastric acid) and released in pH=9 in small intestine environment</h4> | <h4>Lactobacillus was encapsulated into gel sphere which is acid resistant in pH=2 (gastric acid) and released in pH=9 in small intestine environment</h4> | ||
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<span class="w3-center w3-padding-large w3-black w3-xlarge w3-wide w3-animate-opacity">OUR <span class="w3-hide-small">ACHIEVEMENTS</span> </span> | <span class="w3-center w3-padding-large w3-black w3-xlarge w3-wide w3-animate-opacity">OUR <span class="w3-hide-small">ACHIEVEMENTS</span> </span> | ||
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| − | <img src="https://static.igem.org/mediawiki/2017/b/bb/MD-intro-banner-home.png" width="100% | + | <img src="https://static.igem.org/mediawiki/2017/b/bb/MD-intro-banner-home.png" width="100%"> |
<div class="w3-content w3-container w3-padding-64" id="about"> | <div class="w3-content w3-container w3-padding-64" id="about"> | ||
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| − | <div class="col-md- | + | <div class="col-md-12 col-sm-12"> |
| − | <div class="card-header text-white text-center" style="font-size: 1.4rem; background-color:#1dc7d7; letter-spacing: 0.5rem">Project Explained</div> | + | <div class="card-header text-white text-center" style="font-size: 1.4rem; background-color:#1dc7d7; letter-spacing: 0.5rem; margin-top: 3rem">Project Explained</div> |
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| + | <!-- <video width="100%" controls style="border: 1px solid black; border-top: none"> | ||
<source src="https://static.igem.org/mediawiki/2017/2/28/Mingdaochuck1031-2.mp4" type="video/mp4"> | <source src="https://static.igem.org/mediawiki/2017/2/28/Mingdaochuck1031-2.mp4" type="video/mp4"> | ||
| − | </video> | + | </video> --> |
| − | + | </div> | |
| + | <div class="col-md-5 col-sm-5"> | ||
| + | <blockquote style="font-size: 1.3rem"> | ||
| + | Sugar makes you happy but you may have a concern for obesity and diabetes. This year we “crushed” the sugar before absorbed into the small intestine using a genetically engineered microbe with a facilitated glucose transporter system. Until running out of the glucose, a suicide circuit in a glucose responsive repressor system will be triggered to lyse bacterial cells by lysis proteins and degrade gDNA by nucleases. We also created a Lactobacillus recombination vector with standard BioBrick assembly sites for stably transforming probiotics through chromosomal homologous recombination.<br> | ||
| + | <i class="fa fa-facebook" aria-hidden="true"></i> Learn more at our <a href="https://www.facebook.com/mingdaoigem" style="text-decoration: none; color: blue">Facebook</a> | ||
| + | </blockquote> | ||
| + | </div> | ||
</div> | </div> | ||
Latest revision as of 13:47, 15 December 2017
SUGAR
WHAT IS
- Probiotic-based sugar retrieval system
Project Slideshow
Glucose Transporter Device
Glucose transporter with high efficiency was cloned from Salmonella which enhanced the glucose absorption in the engineered E. coli.
Suicide Circuit
The suicide process was induced upon the loss of glucose which extends the functions of the existing parts glucose response promoter, repressor system, lysis and nuclease genes
Probiotic System
A novel homologous recombination vector for transforming Lactobacillus was created and designed based on the standard BioBrick assembly rule
Modeling & Acid-Resistant Pill
Lactobacillus was encapsulated into gel sphere which is acid resistant in pH=2 (gastric acid) and released in pH=9 in small intestine environment
Prototype & Applied Design
simulate a pill passing through esophagus (pH=7) and stomach (pH=2) to the small intestine (pH=9) in the digestive system.
OUR
- 1.5 Years - 18 comrades -
Not limitted to the Lab,
We dream big,
Achievement
- 1.5 Years - 18 comrades -
See how much We've achieved!
Human Practice
Not limitted to the Lab,
we make dreams into reality
Project
We dream big,
with substantial SynBio study
Project Explained
Sugar makes you happy but you may have a concern for obesity and diabetes. This year we “crushed” the sugar before absorbed into the small intestine using a genetically engineered microbe with a facilitated glucose transporter system. Until running out of the glucose, a suicide circuit in a glucose responsive repressor system will be triggered to lyse bacterial cells by lysis proteins and degrade gDNA by nucleases. We also created a Lactobacillus recombination vector with standard BioBrick assembly sites for stably transforming probiotics through chromosomal homologous recombination.
Learn more at our Facebook
© iGEM Mingdao 2017. Design: Kevin Li. All rights reserved.
