Difference between revisions of "Team:Lambert GA/Hardware"

 
(33 intermediate revisions by 4 users not shown)
Line 88: Line 88:
 
#MainTitle {
 
#MainTitle {
 
padding-top: 50px;
 
padding-top: 50px;
−
padding-bottom: 7px;
+
padding-bottom: 20px;
 
font-size: 45px;
 
font-size: 45px;
 
color: #D49AE6; }
 
color: #D49AE6; }
Line 335: Line 335:
 
       <a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a>
 
       <a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a>
 
       <div class="dropdown-content">
 
       <div class="dropdown-content">
−
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part_Collection</a>
+
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;"  
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;"  
 
href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a>
 
href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a>
Line 356: Line 356:
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a>
−
      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Measurement">Measurement</a>
 
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a>
Line 373: Line 372:
  
 
<br>
 
<br>
−
 
 
<center> <h1 id="MainTitle"><b> Hardware </b></h1>  
 
<center> <h1 id="MainTitle"><b> Hardware </b></h1>  
 +
 
<img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;">
 
<img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;">
 
</center>
 
</center>
  
−
 
+
<br>
 
<center><h3 style="color: #D49AE6;">Function</h3></center>
 
<center><h3 style="color: #D49AE6;">Function</h3></center>
−
<p style="width: 100%; margin: auto; font-size: 16px;">The Chrome-Q Light Chamber is a 3D-printed imaging measurement system used to quantify results of nutrient deficiency. For our current project, it is also used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression.  
+
<br>
 +
<p style="width: 100%; margin: auto; font-size: 20px; text-indent:50px;;">The Chrome-Q Light Chamber is a 3D-printed imaging measurement system used to quantify chromoproteins/color-based data. For our current project, it is used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression.  
 
</p></center>
 
</p></center>
 
<br>
 
<br>
 
<div class="center">
 
<div class="center">
−
<img class="img-responsive" style="width:600px" src="https://static.igem.org/mediawiki/2017/b/b2/T--LambertGA--camera2.jpg">
 
 
</div>
 
</div>
 
<br>
 
<br>
 
<center><h3 style="color: #D49AE6;">Development</h3></center>
 
<center><h3 style="color: #D49AE6;">Development</h3></center>
−
<p style="width: 100%; margin: auto; font-size: 16px;">One of the most critical variables in imaging today is the consistency of light. Different wavelengths, intensity, power, wattage, and even angling of lights can affect the result of camera imaging. To this end, the Chrome-Q  is designed to control these variables of light. The dimensions and dome shape have been optimized for ideal imaging distance and lighting amount. A series circuit was used in order to disrtibute full and equal voltage to each LED in the Chrome-Q. This flowchart explains our process/iterations.</p><center><img src="https://static.igem.org/mediawiki/2017/5/5b/T--Lambert_GA--ChromeQFlowchart.png" style="height: 550px;"></center>
+
<br>
 +
<p style="width: 100%; margin: auto; font-size: 20px; text-indent:50px;;">One of the most critical variables in imaging today is the consistency of light. Different wavelengths, intensity, power, wattage, and even angling of lights can affect the result of camera imaging. To this end, the Chrome-Q  is designed to control these variables of light. The dimensions and dome shape have been optimized for ideal imaging distance and lighting amount. A parallel circuit was used in order to distribute full and equal voltage to each LED in the Chrome-Q. This flowchart explains our process/iterations.</p><center><img src="https://static.igem.org/mediawiki/2017/archive/7/7d/20171101161027%21T--Lambert_GA--EngineeringProcessChromeQ.png" style="height: 550px;"></center>
 
<br>
 
<br>
 
<center><h3 style="color: #D49AE6;">On-Site Application</h3></center>
 
<center><h3 style="color: #D49AE6;">On-Site Application</h3></center>
−
<p style="width: 100%; margin: auto; font-size: 16px;">To use the Chrome-Q, first a sample must be placed on the base under the center of the dome. Then, using the app, a phone can take a picture of the sample through the eyehole, and calculate degradation of protein by analyzing the RGB values in the pixels of the samples.</p>
+
<br>
 +
<p style="width: 100%; margin: auto; font-size: 20px; text-indent:50px;">To use the Chrome-Q, first a sample must be placed in the wells in the base under the dome. Then, using the app, a phone can take a picture of the sample through the eyehole, and calculate degradation of protein by analyzing the saturation and hue values derived from the RGB values in the pixels of the samples.</p>
 
<br>
 
<br>
 
<center><h3 style="color: #D49AE6;">Inexpensive</h3></center>
 
<center><h3 style="color: #D49AE6;">Inexpensive</h3></center>
−
<p style="width: 100%; margin: auto; font-size: 16px;">As a high school lab, one of the many problems we face in research is the lack of funding and monetary support. Sophisticated equipment is incredibly expensive, and so it is very hard to afford the necessary tools and measurement systems for our research. The Chrome-Q Light Chamber is designed to conquer the issue of the cost of a fluorimeter – instead of visualizing with fluorescence, the project uses chromoproteins, which can then be imaged with the chamber we built, and results can be obtained that way. This technology can be used by other teams and facilities for their research, and the full plan of the design is available below so that any team can build it themselves. </p>
+
<br>
 +
<p style="width: 100%; margin: auto; font-size: 20px; text-indent:50px;;">As a high school lab, one of the many problems we face in research is the lack of funding and financial support. Sophisticated equipment is incredibly expensive, and so it is very hard to afford or even gain access to the necessary tools and measurement systems for our research, such as plate readers and fluorimeters, that we use to measure our circuits expression. The Chrome-Q Dome is designed to conquer the issue of the cost of a fluorimeter – instead of visualizing with fluorescence, the project uses RGB and HSV values, which can then be imaged with the chamber we built, and results can be obtained. This technology can be used by other teams and facilities for their research, and the full plan of the design is available below so that any team can build it themselves. The materials needs for the Chrome-Q costs around $100 and is easily assembled, while plate readers and fluorimeters can easily cost upwards of $12,000. </p>
 
<br>
 
<br>
 
<center><h3 style="color: #D49AE6;">Build Guide</h3></center>
 
<center><h3 style="color: #D49AE6;">Build Guide</h3></center>
 +
<br>
 +
<center>The files can be downloaded <a style="color: #D49AE6;" href="https://static.igem.org/mediawiki/2017/0/05/T--Lambert_GA--Chrome-QFiles.zip">HERE</a>.</center>
 
<p style="width: 100%; margin: auto; font-size: 16px;">  
 
<p style="width: 100%; margin: auto; font-size: 16px;">  
 
<b style="font-size: 20px;">Tools</b>
 
<b style="font-size: 20px;">Tools</b>
 
<p style="width: 100%; margin: auto; font-size: 16px;">1. 3D Printer (Build Area of at least 7 x 7 x 8in)
 
<p style="width: 100%; margin: auto; font-size: 16px;">1. 3D Printer (Build Area of at least 7 x 7 x 8in)
 
<br>
 
<br>
−
2. Non-Reflective Spray Paint(Optional)
+
2. Non-Reflective Spray Paint
 
<br>
 
<br>
 
3. Camera </p><br><br>
 
3. Camera </p><br><br>
Line 408: Line 412:
 
<p style="width: 100%; margin: auto; font-size: 16px;">1. All the STL files included within the file.
 
<p style="width: 100%; margin: auto; font-size: 16px;">1. All the STL files included within the file.
 
<br>
 
<br>
−
2. LED Lights(3)
+
2. LED Lights(3, G4 LED Bulb - 2 Watt (20 Watt Equivalent) Bi-Pin LED Disc - White - 170 Lumens - Natural White)
 
<br>
 
<br>
 
3. 9-Volt batteries(2)<br>
 
3. 9-Volt batteries(2)<br>
−
4. Wire
+
4. Wire (18-22 gauge, at personal discretion)
 +
<br>
 +
5. 3 Ohm Resistor(2)
 
</p>
 
</p>
 +
<br><br><br>
 +
<h3 style="color: #D49AE6;"><center>Circuit Design</center></h3>
 +
<br>
 +
<center><img src="https://static.igem.org/mediawiki/2017/6/67/T--Lambert_GA--CircuitDesign.png" width="400px;">
 +
<br>
 +
<i style="font-size: 14px; color: white;"> Circuit design layout for the Chrome-Q system </i></center>
 
<br>
 
<br>
−
 
 
<b>Tips</b>
 
<b>Tips</b>
−
<p style="width: 100%; margin: auto; font-size: 16px;">For best results, print at a slow speed to ensure the best outcome possible and make sure the 3D printer is tuned correctly. Print each of the Parts at any desired infill percentage and layer height. Also, it’s recommended to print with brims so it does not curl when printing. If need be, the SLDPRT versions are included in the file. </p>
+
<p style="width: 100%; margin: auto; font-size: 20px; text-indent:50px;">For best results, print at a slow speed to ensure the best outcome possible and make sure the 3D printer is tuned correctly. Print each of the Parts at any desired infill percentage and layer height. Also, it’s recommended to print with brims so it does not curl when printing. If need be, the SLDPRT versions are included in the file. Be sure to wire the circuit with the batteries in a daisy-chain, with the resistors placed after the power source. The design will work if powered by a outlet, we used batteries to power the circuit in order to make it easily portable.</p>
 
<br><br>
 
<br><br>
  
Line 430: Line 441:
 
8. Sand any defects <br>
 
8. Sand any defects <br>
 
9. Test that the dome fits on base. <br>
 
9. Test that the dome fits on base. <br>
−
10. Build series circuit with batteries and LEDs. <br>
+
10. Build parallel circuit with batteries and LEDs. <br>
 
11. Align circuit such that LEDs fit into slots. <br>
 
11. Align circuit such that LEDs fit into slots. <br>
  

Latest revision as of 14:09, 15 December 2017


Hardware


Function


The Chrome-Q Light Chamber is a 3D-printed imaging measurement system used to quantify chromoproteins/color-based data. For our current project, it is used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression.



Development


One of the most critical variables in imaging today is the consistency of light. Different wavelengths, intensity, power, wattage, and even angling of lights can affect the result of camera imaging. To this end, the Chrome-Q is designed to control these variables of light. The dimensions and dome shape have been optimized for ideal imaging distance and lighting amount. A parallel circuit was used in order to distribute full and equal voltage to each LED in the Chrome-Q. This flowchart explains our process/iterations.


On-Site Application


To use the Chrome-Q, first a sample must be placed in the wells in the base under the dome. Then, using the app, a phone can take a picture of the sample through the eyehole, and calculate degradation of protein by analyzing the saturation and hue values derived from the RGB values in the pixels of the samples.


Inexpensive


As a high school lab, one of the many problems we face in research is the lack of funding and financial support. Sophisticated equipment is incredibly expensive, and so it is very hard to afford or even gain access to the necessary tools and measurement systems for our research, such as plate readers and fluorimeters, that we use to measure our circuits expression. The Chrome-Q Dome is designed to conquer the issue of the cost of a fluorimeter – instead of visualizing with fluorescence, the project uses RGB and HSV values, which can then be imaged with the chamber we built, and results can be obtained. This technology can be used by other teams and facilities for their research, and the full plan of the design is available below so that any team can build it themselves. The materials needs for the Chrome-Q costs around $100 and is easily assembled, while plate readers and fluorimeters can easily cost upwards of $12,000.


Build Guide


The files can be downloaded HERE.

Tools

1. 3D Printer (Build Area of at least 7 x 7 x 8in)
2. Non-Reflective Spray Paint
3. Camera



Parts

1. All the STL files included within the file.
2. LED Lights(3, G4 LED Bulb - 2 Watt (20 Watt Equivalent) Bi-Pin LED Disc - White - 170 Lumens - Natural White)
3. 9-Volt batteries(2)
4. Wire (18-22 gauge, at personal discretion)
5. 3 Ohm Resistor(2)




Circuit Design



Circuit design layout for the Chrome-Q system

Tips

For best results, print at a slow speed to ensure the best outcome possible and make sure the 3D printer is tuned correctly. Print each of the Parts at any desired infill percentage and layer height. Also, it’s recommended to print with brims so it does not curl when printing. If need be, the SLDPRT versions are included in the file. Be sure to wire the circuit with the batteries in a daisy-chain, with the resistors placed after the power source. The design will work if powered by a outlet, we used batteries to power the circuit in order to make it easily portable.



Instructions

1. Transfer all the STL files into preferred slicing software.
2. Change settings accordingly to the printer.
3. Orient the parts where it can be printed easiest.
4. Convert it into GCODE files.
5. Transfer the GCODE files into preferred printing software.
6. Start Printing.

7. Carefully remove the prints.
8. Sand any defects
9. Test that the dome fits on base.
10. Build parallel circuit with batteries and LEDs.
11. Align circuit such that LEDs fit into slots.
*Note: If you are interested in 3D-printing the Chrome-Q Light Chamber, please contact us at igem.lamb@gmail.com for the program print files.


References

Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2017, from http://www.colorcodepicker.com/

Purple color codes. (n.d.). Retrieved October 10, 2017, from http://www.rapidtables.com/web/color/purple-color.htm

RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2017, from http://www.perbang.dk/rgbgradient/

Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x