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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Capture part</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Capture part</h3> | ||
<div> | <div> | ||
− | <h4><strong>1.1 SDS-PAGE verification for <I> | + | <h4><strong>1.1 SDS-PAGE verification for <I>oprF-3*LBT 4</I> & <I>oprF-Sitag</I> expression</strong> </h4> |
<p>Whether the oprF-3*LBT or oprF-Sitag can be expressed or not needs to be detected by SDS-PAGE.</p> | <p>Whether the oprF-3*LBT or oprF-Sitag can be expressed or not needs to be detected by SDS-PAGE.</p> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | ||
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<div class="col-xs-12"> | <div class="col-xs-12"> | ||
<h4><strong>1.3 Verification of Tb3+ capturing by precipitation titration of remained Tb3+</strong> </h4> | <h4><strong>1.3 Verification of Tb3+ capturing by precipitation titration of remained Tb3+</strong> </h4> | ||
− | <p> | + | <p>To measure REEBOT’s ability to capture Tb ions, one kind of lanthanide ions, we have done a precipitation titration of Tb hydroxide. We first add REEBOT into Tb ion solution. Each of them has certain concentration and volume. After incubation, we discard the bacteria, and then titrate the solution with sodium hydroxide. The less precipitation of Tb hydroxide generates, the more Tb ions are captured by the bacteria. </p> |
<img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b1/2017_HUST_China_demostrate005.png" class="col-xs-3 col-xs-offset-1" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b1/2017_HUST_China_demostrate005.png" class="col-xs-3 col-xs-offset-1" style="padding: 10px 0px;"> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/6/64/2017_HUST_China_demostrate006.png" class="col-xs-4 col-xs-offset-2" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/6/64/2017_HUST_China_demostrate006.png" class="col-xs-4 col-xs-offset-2" style="padding: 10px 0px;"> |
Latest revision as of 17:45, 15 December 2017