Difference between revisions of "Team:Munich"

 
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<html>
 
<html>
 
<head>
 
<head>
 +
<link rel="stylesheet" type="text/css"
 +
href="https://2017.igem.org/Template:Munich/CSS?action=raw&ctype=text/css" />
 +
<link rel="stylesheet" type="text/css"
 +
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
<style>
 
<style>
−
/* Generated by http://css.spritegen.com CSS Sprite Generator */
+
body {line-height: initial;}
−
+
h1, h2,h3 {
−
.wiki-image-menu-achievements-high, .wiki-image-menu-achievements-low, .wiki-image-menu-application-high, .wiki-image-menu-application-low, .wiki-image-menu-attributions-high,
+
  color: #51a7f9 !important;
−
.wiki-image-menu-attributions-low, .wiki-image-menu-cascaid-high, .wiki-image-menu-cascaid-low, .wiki-image-menu-collaboratinginstitutions-high, .wiki-image-menu-collaboratinginstitutions-low,
+
  text-align: center !important;
−
.wiki-image-menu-collaborations-high, .wiki-image-menu-collaborations-low, .wiki-image-menu-demonstration-high, .wiki-image-menu-demonstration-low, .wiki-image-menu-description-high,
+
−
.wiki-image-menu-description-low, .wiki-image-menu-design-high, .wiki-image-menu-design-low, .wiki-image-menu-entrepreneurship-high, .wiki-image-menu-entrepreneurship-low,
+
−
.wiki-image-menu-finalresults-high, .wiki-image-menu-finalresults-low, .wiki-image-menu-hardware-high, .wiki-image-menu-hardware-low, .wiki-image-menu-hp-high,
+
−
.wiki-image-menu-hp-low, .wiki-image-menu-hpsilver-high, .wiki-image-menu-hpsilver-low, .wiki-image-menu-ihpgold-high, .wiki-image-menu-ihpgold-low,
+
−
.wiki-image-menu-interlab-high, .wiki-image-menu-interlab-low, .wiki-image-menu-labjournal-high, .wiki-image-menu-labjournal-low, .wiki-image-menu-materials-high,
+
−
.wiki-image-menu-materials-low, .wiki-image-menu-model-high, .wiki-image-menu-model-low, .wiki-image-menu-parts-high, .wiki-image-menu-parts-low,
+
−
.wiki-image-menu-pis-high, .wiki-image-menu-pis-low, .wiki-image-menu-project-high, .wiki-image-menu-project-low, .wiki-image-menu-proofofconcept-high,
+
−
.wiki-image-menu-proofofconcept-low, .wiki-image-menu-protocolsmethods-high, .wiki-image-menu-protocolsmethods-low, .wiki-image-menu-publicengagement-high, .wiki-image-menu-publicengagement-low,
+
−
.wiki-image-menu-safety-high, .wiki-image-menu-safety-low, .wiki-image-menu-softhardware-high, .wiki-image-menu-softhardware-low, .wiki-image-menu-software-high,
+
−
.wiki-image-menu-software-low, .wiki-image-menu-sponsors-high, .wiki-image-menu-sponsors-low, .wiki-image-menu-supervisors-high, .wiki-image-menu-supervisors-low,
+
−
.wiki-image-menu-team-high, .wiki-image-menu-team-low, .wiki-image-menu-teammembers-high, .wiki-image-menu-teammembers-low, .wiki-image-menu-wetlab-high,
+
−
.wiki-image-menu-wetlab-low
+
−
{ display: inline-block; background: url('https://static.igem.org/mediawiki/2017/d/df/T--Munich--menu-sprite.png') no-repeat; overflow: hidden; text-indent: -9999px; text-align: left; }
+
−
+
−
.wiki-image-menu-achievements-high { background-position: -0px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-achievements-low { background-position: -137px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-application-high { background-position: -274px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-application-low { background-position: -411px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-attributions-high { background-position: -548px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-attributions-low { background-position: -685px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-cascaid-high { background-position: -822px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-cascaid-low { background-position: -959px -0px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-collaboratinginstitutions-high { background-position: -0px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-collaboratinginstitutions-low { background-position: -137px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-collaborations-high { background-position: -274px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-collaborations-low { background-position: -411px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-demonstration-high { background-position: -548px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-demonstration-low { background-position: -685px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-description-high { background-position: -822px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-description-low { background-position: -959px -32px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-design-high { background-position: -0px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-design-low { background-position: -137px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-entrepreneurship-high { background-position: -274px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-entrepreneurship-low { background-position: -411px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-finalresults-high { background-position: -548px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-finalresults-low { background-position: -685px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hardware-high { background-position: -822px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hardware-low { background-position: -959px -64px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hp-high { background-position: -0px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hp-low { background-position: -137px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hpsilver-high { background-position: -274px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-hpsilver-low { background-position: -411px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-ihpgold-high { background-position: -548px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-ihpgold-low { background-position: -685px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-interlab-high { background-position: -822px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-interlab-low { background-position: -959px -96px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-labjournal-high { background-position: -0px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-labjournal-low { background-position: -137px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-materials-high { background-position: -274px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-materials-low { background-position: -411px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-model-high { background-position: -548px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-model-low { background-position: -685px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-parts-high { background-position: -822px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-parts-low { background-position: -959px -128px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-pis-high { background-position: -0px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-pis-low { background-position: -137px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-project-high { background-position: -274px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-project-low { background-position: -411px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-proofofconcept-high { background-position: -548px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-proofofconcept-low { background-position: -685px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-protocolsmethods-high { background-position: -822px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-protocolsmethods-low { background-position: -959px -160px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-publicengagement-high { background-position: -0px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-publicengagement-low { background-position: -137px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-safety-high { background-position: -274px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-safety-low { background-position: -411px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-softhardware-high { background-position: -548px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-softhardware-low { background-position: -685px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-software-high { background-position: -822px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-software-low { background-position: -959px -192px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-sponsors-high { background-position: -0px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-sponsors-low { background-position: -137px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-supervisors-high { background-position: -274px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-supervisors-low { background-position: -411px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-team-high { background-position: -548px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-team-low { background-position: -685px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-teammembers-high { background-position: -822px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-teammembers-low { background-position: -959px -224px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-wetlab-high { background-position: -0px -256px; width: 137px; height: 32px; }
+
−
.wiki-image-menu-wetlab-low { background-position: -137px -256px; width: 137px; height: 32px; }
+
−
 
+
−
 
+
−
 
+
−
 
+
−
#home_logo, #sideMenu { display:none; }
+
−
    #sideMenu, #top_title, .patrollink  {display:none;}
+
−
    #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;}
+
−
    body {background-color:white; font-size: 14px; }
+
−
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
−
 
+
−
 
+
−
#HQ_page #myFooter td{
+
−
  vertical-align: text-top;
+
 
}
 
}
  
−
#HQ_page td not(#myContent){
+
/*Time for the CSS*/
−
padding: 0;
+
* {margin: 0; padding: 0;}
−
}
+
  
−
#HQ_page table{
+
.slider{
−
font-size: 14px;
+
width: 900px; /*Same as width of the large image*/
 +
position: relative;
 +
/*Instead of height we will use padding*/
 +
padding-top: 600px; /*That helps bring the labels down*/
 +
 +
/*Lets add a shadow*/
 
}
 
}
  
−
hr{
 
−
height: 1px !important;
 
−
}
 
−
#HQ_page td{
 
−
border-style: none ;
 
−
vertical-align: middle;
 
−
}
 
  
−
#HQ_page table{
+
/*Last thing remaining is to add transitions*/
−
border-style: none !important;
+
.slider>img{
−
margin: 0 !important;
+
position: absolute;
 +
left: 0; top: 0;
 +
transition: all 0.5s;
 
}
 
}
  
−
html, body
+
.slider input[name='slide_switch'] {
−
{
+
display: none;
−
margin: 0;
+
−
padding: 0;
+
−
font-family: "Helvetica", Arial, Serif;
+
−
}
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−
.dropdown {
+
−
    width: 137px;
+
−
    margin: auto;
+
−
    position: relative;
+
−
    display: inline-block;
+
 
}
 
}
  
−
 
+
.slider label {
−
 
+
/*Lets add some spacing for the thumbnails*/
−
.dropbtnCascAid {
+
margin: 30px 0 0 18px;
−
    position: relative;
+
−
    padding: 0px;
+
float: left;
−
    border: none;
+
cursor: pointer;
−
    cursor: pointer;
+
transition: all 0.5s;
−
    width: 137px;
+
−
    height: 32px;
+
/*Default style = low opacity*/
 +
opacity: 0.6;
 +
 +
        z-index: 99;
 
}
 
}
−
.dropdown:hover .dropbtnCascAid {
 
−
    background-image: url("https://static.igem.org/mediawiki/2017/1/1f/T--Munich--wiki_image_menu_cascaid_high.gif");
 
−
}
 
−
 
−
 
  
−
.dropbtnProject {
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.slider label img{
−
    background-image: url("https://static.igem.org/mediawiki/2017/b/ba/T--Munich--wiki_image_menu_project_low.gif");
+
display: block;
−
    position: relative;
+
−
    padding: 0px;
+
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
 
}
 
}
−
.dropdown:hover .dropbtnProject {
 
−
    background-image: url("https://static.igem.org/mediawiki/2017/5/5d/T--Munich--wiki_image_menu_project_high.gif");
 
−
}
 
−
 
−
 
  
−
.dropbtnWetlab {
+
/*Time to add the click effects*/
−
    background-image: url("https://static.igem.org/mediawiki/2017/9/9d/T--Munich--wiki_image_menu_wetlab_low.gif");
+
.slider input[name='slide_switch']:checked+label {
−
    position: relative;
+
border-color: #666;
−
    padding: 0px;
+
opacity: 1;
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
 
}
 
}
−
.dropdown:hover .dropbtnWetlab {
+
/*Clicking any thumbnail now should change its opacity(style)*/
−
    background-image: url("https://static.igem.org/mediawiki/2017/e/e1/T--Munich--wiki_image_menu_wetlab_high.gif");
+
/*Time to work on the main images*/
 +
.slider input[name='slide_switch'] ~ img {
 +
opacity: 0;
 +
transform: scale(1.1);
 
}
 
}
−
 
+
/*That hides all main images at a 110% size
−
 
+
On click the images will be displayed at normal size to complete the effect
−
 
+
*/
−
.dropbtnSoftHardware {
+
.slider input[name='slide_switch']:checked+label+img {
−
    background-image: url("https://static.igem.org/mediawiki/2017/b/bb/T--Munich--wiki_image_menu_softhardware_low.gif");
+
opacity: 1;
−
    position: relative;
+
transform: scale(1);
−
    padding: 0px;
+
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
−
}
+
−
.dropdown:hover .dropbtnSoftHardware {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/1/18/T--Munich--wiki_image_menu_softhardware_high.gif");
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropbtnModel {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/7/7b/T--Munich--wiki_image_menu_model_low.gif");
+
−
    position: relative;
+
−
    padding: 0px;
+
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
−
}
+
−
.dropdown:hover .dropbtnModel {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/3/3d/T--Munich--wiki_image_menu_model_high.gif");
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropbtnHP {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/7/78/T--Munich--wiki_image_menu_hp_low.gif");
+
−
    position: relative;
+
−
    padding: 0px;
+
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
−
}
+
−
.dropdown:hover .dropbtnHP {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_menu_hp_high.gif");
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropbtnTeam {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_menu_team_low.gif");
+
−
    position: relative;
+
−
    padding: 0px;
+
−
    border: none;
+
−
    cursor: pointer;
+
−
    width: 137px;
+
−
    height: 32px;
+
−
}
+
−
.dropdown:hover .dropbtnTeam {
+
−
    background-image: url("https://static.igem.org/mediawiki/2017/5/57/T--Munich--wiki_image_menu_team_high.gif");
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropdown-contentDescription {
+
−
    background-image: url("wiki_image_menu_description_low.gif");
+
−
    display: none;
+
−
    position: absolute;
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    z-index: 1;
+
−
}
+
−
.dropdown:hover .dropdown-contentDescription {
+
−
    background-image: url("wiki_image_menu_description_high.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    display: block;
+
−
}
+
−
.dropdown-contentDescription a {
+
−
    background-image: url("wiki_image_menu_description_low.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    padding: 0px 0px;
+
−
    text-decoration: none;
+
−
    display: block;
+
−
}
+
−
.dropdown-contentDescription a:hover {
+
−
    background-image: url("wiki_image_menu_description_high.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropdown-contentDesign {
+
−
    background-image: url("wiki_image_menu_design_low.gif");
+
−
    display: none;
+
−
    position: absolute;
+
−
    top: 64;
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    z-index: 1;
+
−
}
+
−
.dropdown:hover .dropdown-contentDesign {
+
−
    background-image: url("wiki_image_menu_design_high.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    display: block;
+
−
}
+
−
.dropdown-contentDesign a {
+
−
    background-image: url("wiki_image_menu_design_low.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    padding: 0px 0px;
+
−
    text-decoration: none;
+
−
    display: block;
+
−
}
+
−
.dropdown-contentDesign a:hover {
+
−
    background-image: url("wiki_image_menu_design_high.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.dropdown-contentProofofConcept {
+
−
    background-image: url("wiki_image_menu_proofofconcept_low.gif");
+
−
    display: none;
+
−
    position: absolute;
+
−
    top: 96;
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    z-index: 1;
+
−
}
+
−
.dropdown:hover .dropdown-contentProofofConcept {
+
−
    background-image: url("wiki_image_menu_proofofconcept_high.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    display: block;
+
−
}
+
−
.dropdown-contentProofofConcept a {
+
−
    background-image: url("wiki_image_menu_proofofconcept_low.gif");
+
−
    height: 32px;
+
−
    width: 137px;
+
−
    padding: 0px 0px;
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/*Clicking on any thumbnail now should activate the image related to it*/
  
 +
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{{Munich/Menu}}
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<source src="https://static.igem.org/mediawiki/2017/4/44/T--Munich--FrontPageVideo.mp4" type="video/mp4">
 
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<table width="900" border=0 cellspacing=0 cellpadding=10>
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−
<font size=7 color=#444444><b>Abstract</b></font>
+
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 +
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 +
<font size=7 ><b style="color: #51a7f9">Abstract</b></font>
 
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<p style="text-align:justify">
 
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−
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
+
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from <i>E. coli</i>. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
 
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+
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+
 
<font size=3 color=#444444>
 
<font size=3 color=#444444>
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
−
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−
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Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
</font>
 
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−
<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
+
<tr><td colspan=6 align=middle valign=center>
−
<font size=2>| The iGEM Munich 2017 Team | 2017.07.13 |</font><br><br><br><center><hr></center>
+
<font size=10 ><b style="color: #51a7f9; font-size: 60px; margin-top: 20px">Awards & Achievements</b></font>
 
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−
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+
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+
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+
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+
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+
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+
−
<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
+
−
<img src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_coop_lmu_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg'">
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+
 
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−
 
+
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−
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+
<tr>
−
<a href="https://www.tum.de/" target="_blank4" >
+
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−
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+
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−
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+
<h1>First Runner-up</h1>
 +
</div>
 
</td>
 
</td>
−
 
+
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−
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+
<div class="captionPicture">
−
<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
+
<h1>Best Diagnostics Project</h1>
−
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/72/T--Munich--wiki_image_coop_e14_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg'">
+
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−
</a>
+
 
</td>
 
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 +
</tr>
  
−
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+
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−
<a href="http://www.biochem.mpg.de/en" target="_blank5" >
+
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−
<img src="https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg'">
+
<div class="captionPicture">
−
</a>
+
<h2>Best Model</h2>
 +
</div>
 
</td>
 
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−
 
+
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+
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−
<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
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<h3> <a style="color: #51a7f9 !important" href="/Team:Munich/Gallery">See the rest of our gallery...</a></h3>
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Latest revision as of 20:49, 15 December 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics. Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

Awards & Achievements

First Runner-up

Best Diagnostics Project

Best Model

Best Applied Design

Best Hardware

Best Software

Nominated for Best Wiki

Nominated for Best Presentation

Nominated for Best Poster

Nominated for Best Integrated Human Practices

Nominated for Best Education & Public Engagement

Nominated for Best Measurement

Nominated for Best Composite Part

Nominated for Best Part Collection
Gallery

See the rest of our gallery...