Difference between revisions of "Team:Munich"

 
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<style>
 
<style>
 
body {line-height: initial;}
 
body {line-height: initial;}
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h1, h2,h3 {
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color: #51a7f9 !important;
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text-align: center !important;
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/*Time for the CSS*/
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* {margin: 0; padding: 0;}
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width: 900px; /*Same as width of the large image*/
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position: relative;
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/*Instead of height we will use padding*/
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padding-top: 600px; /*That helps bring the labels down*/
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/*Lets add a shadow*/
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}
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/*Last thing remaining is to add transitions*/
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.slider>img{
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position: absolute;
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left: 0; top: 0;
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transition: all 0.5s;
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display: none;
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.slider label {
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/*Lets add some spacing for the thumbnails*/
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margin: 30px 0 0 18px;
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float: left;
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cursor: pointer;
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transition: all 0.5s;
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/*Default style = low opacity*/
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.slider label img{
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display: block;
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}
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border-color: #666;
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/*Clicking any thumbnail now should change its opacity(style)*/
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/*Time to work on the main images*/
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.slider input[name='slide_switch'] ~ img {
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/*That hides all main images at a 110% size
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On click the images will be displayed at normal size to complete the effect
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*/
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.slider input[name='slide_switch']:checked+label+img {
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transform: scale(1);
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/*Clicking on any thumbnail now should activate the image related to it*/
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<!-- Head End -->
 
<!-- Head End -->
 
<!-- Content Begin -->
 
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<video height="270" width="960" autoplay="autoplay">
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<video width="980" autoplay="autoplay" controls>
 
<source src="https://static.igem.org/mediawiki/2017/4/44/T--Munich--FrontPageVideo.mp4" type="video/mp4">
 
<source src="https://static.igem.org/mediawiki/2017/4/44/T--Munich--FrontPageVideo.mp4" type="video/mp4">
 
Your browser does not support the video tag.
 
Your browser does not support the video tag.
 
</video>
 
</video>
 
<table width="900" border=0 cellspacing=0 cellpadding=10>
 
<table width="900" border=0 cellspacing=0 cellpadding=10>
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<tr>
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<font size=7 color=#444444><b>Abstract</b></font>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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<td width=160></td>
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<font size=7 ><b style="color: #51a7f9">Abstract</b></font>
 
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<p style="text-align:justify">
 
<p style="text-align:justify">
 
<font size=3 color=#444444>
 
<font size=3 color=#444444>
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The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
+
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from <i>E. coli</i>. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
 
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<p style="text-align:justify">
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<font size=3 color=#444444>
 
<font size=3 color=#444444>
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
 
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
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</p>
 
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<p style="text-align:justify">
 
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<font size=3 color=#444444>
 
 
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
 
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</font>
 
</p>
 
</p>
 
</td></tr>
 
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<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
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<tr><td colspan=6 align=middle valign=center>
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<font size=2>| The iGEM Munich 2017 Team | 2017.07.13 |</font><br><br><br><center><hr></center>
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<font size=10 ><b style="color: #51a7f9; font-size: 60px; margin-top: 20px">Awards & Achievements</b></font>
 
</td></tr>
 
</td></tr>
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<tr>
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<img src="https://static.igem.org/mediawiki/2017/e/e5/T--Munich--Banner_Awards.jpg" width=900>
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</td>
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</tr>
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<tr>
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<td align=center valign=center colspan=3>
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<div class="captionPicture">
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<h1>First Runner-up</h1>
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</div>
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</td>
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<td align=center valign=center colspan=3>
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<div class="captionPicture">
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<h1>Best Diagnostics Project</h1>
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</div>
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</td>
 +
</tr>
  
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<tr>
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<td align=center valign=center colspan=3>
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<div class="captionPicture">
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<h2>Best Model</h2>
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</div>
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</td>
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<td align=center valign=center colspan=3>
 +
<div class="captionPicture">
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<h2>Best Applied Design</h2>
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</div>
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</td>
 +
</tr>
  
 +
<tr>
 +
<td align=center valign=center colspan=3>
 +
<div class="captionPicture">
 +
<h2>Best Hardware</h2>
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</div>
 +
</td>
 +
<td align=center valign=center colspan=3>
 +
<div class="captionPicture">
 +
<h2>Best Software</h2>
 +
</div>
 +
</td>
 +
</tr>
  
  
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<tr>
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<td align=center valign=center colspan=2>
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<table id="myFooter" width=960 height=35 cellpadding=0 cellspacing=0 border=0><tr><td align=right class="no-padding" valign=center>
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<h3>Nominated for Best Wiki</h3>
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<table width=300 height=35 cellpadding=0 cellspacing=0 border=0><tr>
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−
 
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<td align=center valign=center>
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<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
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<img src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_coop_lmu_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg'">
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</a>
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</td>
 
</td>
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<td align=center valign=center colspan=2>
−
<td align=center valign=center>
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<h3>Nominated for Best Presentation</h3>
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<a href="https://www.tum.de/" target="_blank4" >
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−
<img src="https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/1/1e/T--Munich--wiki_image_coop_tum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg'">
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−
</a>
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</td>
 
</td>
−
 
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<td align=center valign=center colspan=2>
−
<td align=center valign=center>
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<h3>Nominated for Best Poster</h3>
−
<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
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−
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/72/T--Munich--wiki_image_coop_e14_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg'">
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<tr>
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<a href="http://www.biochem.mpg.de/en" target="_blank5" >
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<img src="https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg'">
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<h3>Nominated for Best Integrated Human Practices</h3>
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</a>
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</td>
 
</td>
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<td align=center valign=center colspan=2>
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<td align=center valign=center>
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<h3>Nominated for Best Education & Public Engagement</h3>
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<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
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<img src="https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6b/T--Munich--wiki_image_coop_helmholtzzentrum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg'">
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<h3>Nominated for Best Measurement</h3>
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<a href="https://igem.org/Main_Page" target="_blank6" >
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<img src="https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_coop_igem_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg'">
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</a>
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</tr>
  
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</tr></table></td><td class="no-padding" align=center valign=center><table width=35 height=35 cellpadding=0 cellspacing=0 border=0><tr><td width="35" align=center valign=center></td></tr></table></td><td class="no-padding" align=left valign=center><table width=200 height=35 cellpadding=0 cellspacing=0 border=0><tr>
 
  
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<td align=center valign=center>
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<tr>
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<a href="https://www.facebook.com/Munich.iGEM" target="_blank7" >
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<td align=center valign=center colspan=1></td>
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<img src="https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/4/44/T--Munich--wiki_image_sm_facebook_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg'">
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<td align=center valign=center colspan=2>
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</a>
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<h3 style="margin-bottom: 50px">Nominated for Best Composite Part</h3>
 
</td>
 
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<td align=center valign=center colspan=2>
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<td align=center valign=center>
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<h3 style="margin-bottom: 50px">Nominated for Best Part Collection</h3>
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<a href="https://twitter.com/iGEM_Munich" target="_blank8" >
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<img src="https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/c/cc/T--Munich--wiki_image_sm_twitter_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg'">
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</a>
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</tr>
  
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<td align=center valign=center>
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<tr><td colspan=6 align=middle valign=center>
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<a href="mailto:igem.munich@gmail.com">
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<font size=10 ><b style="color: #51a7f9; font-size: 60px; margin-top: 20px">Gallery</b></font>
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<img src="https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/9/93/T--Munich--wiki_image_sm_mail_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg'">
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We will make a slider with stylized thumbnails using CSS3
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The markup is very simple:
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Radio Inputs
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Labels with thumbnails to detect click event
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Main Image
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<input type="radio" name="slide_switch" id="id3" checked="checked"/>
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<label for="id3">
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<img src="https://static.igem.org/mediawiki/2017/9/99/T--Munich--Review2017_77.jpeg" width=900/>
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<img src="https://static.igem.org/mediawiki/2017/f/f9/T--Munich--Review2017_13.jpeg" width="100"/>
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<img src="https://static.igem.org/mediawiki/2017/f/f9/T--Munich--Review2017_13.jpeg" width=900/>
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<label for="id7">
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<img src="https://static.igem.org/mediawiki/2017/6/60/T--Munich--Review2017_17.jpeg" width="100"/>
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<img src="https://static.igem.org/mediawiki/2017/6/60/T--Munich--Review2017_17.jpeg" width=900/>
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<h3> <a style="color: #51a7f9 !important" href="/Team:Munich/Gallery">See the rest of our gallery...</a></h3>
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<br><br><br><center><hr></center>
 
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{Munich/Footer}
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{{Munich/Footer}}

Latest revision as of 20:49, 15 December 2017

Description

Design

Demonstrate

Measurement

Applied Design

Final Results

Achievements

Protocols

Notebook

Safety

Parts

Improved Part

Part Collection

InterLab

Model

Software

Collaboration

Detector

Quake Valve

Paperstrips

Sample Processing

Silver

Gold

Engagement

Collaborations

Team members

Sponsors

Attributions

Gallery

Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics. Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

Awards & Achievements

First Runner-up

Best Diagnostics Project

Best Model

Best Applied Design

Best Hardware

Best Software

Nominated for Best Wiki

Nominated for Best Presentation

Nominated for Best Poster

Nominated for Best Integrated Human Practices

Nominated for Best Education & Public Engagement

Nominated for Best Measurement

Nominated for Best Composite Part

Nominated for Best Part Collection
Gallery

See the rest of our gallery...