Difference between revisions of "Team:Berlin diagnostX"

 
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{{Berlin_diagnostX}}
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            <h1 class="text-center igem_blue">The fight against T. solium</h1>
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            <h3 class="text-center">The worldwide fight against Taenia solium
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(pork tapeworm) is facing three different challenges</h3>
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            <img class="img-fluid px-5" src="https://static.igem.org/mediawiki/2017/thumb/7/7c/T--Berlin_diagnostX--icon4.png/600px-T--Berlin_diagnostX--icon4.png">
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            <h5 class="igem_blue text-center mt-4 mb-1 pb-1">HIGH PREVALENCE</h5>
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            <p class="text-center px-5 pt-2">Estimated billions are affected - the infection causing epilepsy, blindness and death</p>
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        </div>
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        <div class="col-4 px-3">
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            <img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/f/f0/T--Berlin_diagnostX--icon3.png/600px-T--Berlin_diagnostX--icon3.png">
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<h5 class="igem_blue text-center mt-4 mb-1 pb-1">INSUFFICIENT DRUG DISTRIBUTION</h5>
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            <p class="text-center px-5 pt-2">Although affordable therapeutics exist, they don't reach patients in infectious hotspots </p>
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        <div class="col-4 px-3">
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            <img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/2/2d/T--Berlin_diagnostX--icon2.png/600px-T--Berlin_diagnostX--icon2.png">
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<h5 class="igem_blue text-center mt-4 mb-1 pb-1">EXPENSIVE DIAGNOSTICS</h5>
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            <p class="text-center px-5 pt-2">No methods suited for field use are yet available preventing the identification of infectious hotspots</p>
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            <h1 class="text-center igem_blue">The vision of diagnost-x</h1>
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            <h4 class="text-center">Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium</h5>
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            <img src="https://static.igem.org/mediawiki/2017/0/0c/T--Berlin_diagnostX--target.png" class="img-fluid">
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            <hr class="igem_seperator mt-0 ml-0">
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            <h5 class="igem_blue pb-1 mb-4">Target Identification</h5>
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            <p class="text-justify mb-1">We sequenced 96 million RNAs  and mapped them against about 12.000 Genes of T. saginata,  T. asiatica and T. solium. The expression level of unique RNAs was analyzed. We identified 215 unique, potential diagnost-x targets.</p>
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<p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p>
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            <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png">
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            <p class="d-inline pl-3"> First RNA isolation from T. solium eggs avoiding hatching procedure </p>
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            <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div>
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            <h5 class="igem_blue pb-1">Sensor Design</h5>
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            <p class="text-justify mb-4">Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.</p>
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            <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png">
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            <p class="d-inline pl-3"> Refined preselection of promising sensors thanks to established software</p>
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            <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div>
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            <img class="img-fluid" src="https://static.igem.org/mediawiki/2017/0/0f/T--Berlin_diagnostX--Switch.gif">
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              <video class="mr-4" width="100%" height="100%" controls>
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                  <source src="https://static.igem.org/mediawiki/2017/6/6f/T--Berlin_diagnostX--pipeline2.mp4" type="video/mp4">
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            <hr class="igem_seperator mt-0 ml-0">
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            <h5 class="igem_blue pb-1">Sensor Screening</h5>
 +
            <p class="text-justify mb-4">The designed switches from the In Silico Team were assembled using Extension PCR. Assembled Sensors passed all through the standard screening in liquid phase.</p>
 +
            <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png">
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            <p class="d-inline pl-3"> 6 successfully working sensors detected thanks to our high flow-through Screening Pipeline</p>
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            <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div>
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            <h5 class="igem_blue pb-1">Considerating future application from Day 1</h5>
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            <p class="text-justify mb-4">Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used - therefore we spared no expense and investigated local circumstances in India.</p>
 +
            <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png">
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            <p class="d-inline pl-3"> Find out more about what the insight we gained in India!</p>
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            <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/HP/Gold_Integrated" role="button">Discover More</a></div>
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            <h5 class="igem_blue pb-1">Raising awareness for T. solium </h5>
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             <p class="text-justify mb-4">Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. Our mission was to raise  awareness for NTDs and provide innovative impulses for the education of a new generation.</p>
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             <p class="d-inline pl-3"> Organisation of a congress with over 150 scientists, politicians & students </p>
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             <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/HP/Silver" role="button">Discover More</a></div>
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            <h5 class="igem_blue pb-1">Route to the Future</h5>
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            <p class="text-justify mb-4">The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time!
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Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!</p>
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            <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png">
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            <p class="d-inline pl-3">Development of an <strong>augmented reality app </strong> for innovative future education</p>
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            <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/AR" role="button">Discover More</a></div>
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         <div class="row"><div class="col-12"><h4 class="text-center igem_blue">Many Thanks to our Sponsors!</h4></div></div>
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{{Berlin_diagnostX2}}

Latest revision as of 01:15, 16 December 2017

The fight against T. solium

The worldwide fight against Taenia solium (pork tapeworm) is facing three different challenges

HIGH PREVALENCE

Estimated billions are affected - the infection causing epilepsy, blindness and death

INSUFFICIENT DRUG DISTRIBUTION

Although affordable therapeutics exist, they don't reach patients in infectious hotspots

EXPENSIVE DIAGNOSTICS

No methods suited for field use are yet available preventing the identification of infectious hotspots

The vision of diagnost-x

Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium

Target Identification

We sequenced 96 million RNAs and mapped them against about 12.000 Genes of T. saginata, T. asiatica and T. solium. The expression level of unique RNAs was analyzed. We identified 215 unique, potential diagnost-x targets.

The colour intensity is proportional to the expression of each identified possible target.

First RNA isolation from T. solium eggs avoiding hatching procedure

Discover More
Sensor Design

Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.

Refined preselection of promising sensors thanks to established software

Discover More
Sensor Screening

The designed switches from the In Silico Team were assembled using Extension PCR. Assembled Sensors passed all through the standard screening in liquid phase.

6 successfully working sensors detected thanks to our high flow-through Screening Pipeline

Discover More
Considerating future application from Day 1

Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used - therefore we spared no expense and investigated local circumstances in India.

Find out more about what the insight we gained in India!

Discover More
Raising awareness for T. solium

Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. Our mission was to raise awareness for NTDs and provide innovative impulses for the education of a new generation.

Organisation of a congress with over 150 scientists, politicians & students

Discover More
Route to the Future

The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!

Development of an augmented reality app for innovative future education

Discover More

Many Thanks to our Sponsors!