Difference between revisions of "Team:USNA Annapolis/Attributions"

Much of our main project was worked on by Seok Park and Cassie Garfola . Seok Park used the Gibson Assembly method to create the genetically engineered machine. The parts consist of the following: A pBBR backbone, pgaA (Our submitted part for IGEM), Green Fluorescent Protein (GFP) Ribosome Binding Site (RBS) GEP, Terminator, pLacZ, nhaR RBS, nhaR (Our second submitted part for IGEM), and lastly another terminator. This was a total of 9 total parts that needed to be stitched into a full construct. This led to the use of the Gibson Assembly method in order to increase the rate of success of creating our construct.

Cassie did most of the work with the ligations and digestion stuff.

On the Web Site

Heading up the wiki page this year is our Computer Science student, Joe Roehm. He produced the wiki using various sources such as CSS and Bootstrap. Many of the images are completely free to use, and the others were created by us. Many of the photos were edited in GIMP, a free image editing software produced by the GNU Project. The website was a continuous effort this year, and Joe kept himself busy, proving to be a valuable part of the USNA team. However, Joe could not have been so successful without the help of the rest of the team, since he stayed mostly out of the lab itself.

We each brought our own particular skillset to the table. It just so happened that I was more familiar with computers than the rest of the gang, so I decided to head up the web development part, and it was a great time!

Our iGEM team last year had been less experienced with building wiki pages, so this year the team made sure to get an early start to achieve an interactive, easy to navigate, and informational webpage for the competition.

InterLab

The InterLab study was conducted by the joint efforts of Natalia Barrow and Dwayne Williams. The two first cultured, then innoculated and grew bacteria colonies according to the interlab studies manual. They then measured various absorbances and flurescences of the bacteria at different times to compare to the results of other labs in the iGem projects to ensure that any future project results could be replicated in various labs around the world.