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</div> | </div> | ||
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+ | |||
+ | |||
+ | <body data-spy="scroll" data-target="#parent"> | ||
+ | <div class="jumbotron"> | ||
+ | <div class="container"> | ||
+ | <h1 style="padding: 0px; margin-bottom: 0px;">Notebook</h1> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-xs-2" id="parent"> | ||
+ | <ul class="nav nav-pills nav-stacked" data-spy="affix" data-offset-top="275" style="overflow-y: auto; height: 100%"> | ||
+ | <li class="active" ><a href="#week1">Week 1</a></li> | ||
+ | <li><a href="#week2">Week 2</a></li> | ||
+ | <li><a href="#week3">Week 3</a></li> | ||
+ | <li><a href="#week4">Week 4</a></li> | ||
+ | <li><a href="#week5">Week 5</a></li> | ||
+ | <li><a href="#week6">Week 6</a></li> | ||
+ | <li><a href="#week7">Week 7</a></li> | ||
+ | <li><a href="#week8">Week 8</a></li> | ||
+ | <li><a href="#week9">Week 9</a></li> | ||
+ | <li><a href="#week10">Week 10</a></li> | ||
+ | <li><a href="#week11">Week 11</a></li> | ||
+ | <li><a href="#week12">Week 12</a></li> | ||
+ | <li><a href="#week13">Week 13</a></li> | ||
+ | <li><a href="#week14">Week 14</a></li> | ||
+ | <li><a href="#week15">Week 15</a></li> | ||
+ | <li><a href="#week16">Week 16</a></li> | ||
+ | <li><a href="#week17">Week 17</a></li> | ||
+ | <li><a href="#week18">Week 18</a></li> | ||
+ | <li><a href="#week19">Week 19</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div class="col-xs-10"> | ||
+ | |||
+ | |||
+ | <!-- Wet Lab Overview | ||
+ | <li class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/6/6f/Cornell-nb-wet.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Wet Lab Overview</h4> | ||
+ | <p> | ||
+ | Our main goals were to 1) clone the three metal transport proteins and pea metallothionein, | ||
+ | 2) assemble the transport proteins and metallothionein to make our composite constructs, | ||
+ | and 3) monitor sequestration efficiency in our <i>in vivo</i> model using <i>E. coli</i>. | ||
+ | </p> | ||
+ | </div> | ||
+ | </li> | ||
+ | --> | ||
+ | <!-- Dry Lab template | ||
+ | <li class="media dry"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/2/28/Cornell-nb-dry.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Dry Lab Overview</h4> | ||
+ | <p> | ||
+ | Text goes here. | ||
+ | </p> | ||
+ | </div> | ||
+ | </li> | ||
+ | --> | ||
+ | <!-- nested template | ||
+ | <li class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/6/6f/Cornell-nb-wet.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Wet Lab Overview</h4> | ||
+ | <p> | ||
+ | Text | ||
+ | </p> | ||
+ | |||
+ | <div class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/2/28/Pb_small.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Lead Subteam</h4> | ||
+ | <p> | ||
+ | Text | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/a/a9/Hg_small.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Mercury Subteam</h4> | ||
+ | <p> | ||
+ | Text | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/d/dd/Ni_small.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Nickel Subteam</h4> | ||
+ | <p> | ||
+ | Text | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/4/40/Cornell_reporters.jpg"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Reporters Subteam</h4> | ||
+ | <p> | ||
+ | Text | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </li> | ||
+ | --> | ||
+ | <!----------------------------- Week 1 --------------------------------------------> | ||
+ | <h3 id="week1">Week 1 (June 2 - June 8)</h3> | ||
+ | <hr> | ||
+ | <ul class="media-list"> | ||
+ | <li class="media"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/6/6f/Cornell-nb-wet.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Wet Lab Overview</h4> | ||
+ | <p> | ||
+ | Transformation efficiency and protocols were tested - heat shock did not work well, and we came to the conclusion to stick with electroporation. | ||
+ | |||
+ | The first day of Wet Lab training began on the 7th! We started working on amplification of metallothionein genes - so far nixA and GST-PMT appear to be working. | ||
+ | </p> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li class="media dry"> | ||
+ | <img class="media object pull-left" src="https://static.igem.org/mediawiki/2014/2/28/Cornell-nb-dry.png"> | ||
+ | <div class="media-body"> | ||
+ | <h4 class="media heading">Dry Lab Overview</h4> | ||
+ | <p> | ||
+ | Today was the first Dry Lab meeting for the new project! We discussed the mechanics and optimization of general filtration systems. We also started looking for the best way to make hollow fiber reactors the focal point of our filtration system. | ||
+ | </p> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <br><br> |
Revision as of 15:46, 26 July 2017
Notebook
Week 1 (June 2 - June 8)
-
Wet Lab Overview
Transformation efficiency and protocols were tested - heat shock did not work well, and we came to the conclusion to stick with electroporation. The first day of Wet Lab training began on the 7th! We started working on amplification of metallothionein genes - so far nixA and GST-PMT appear to be working.
-
Dry Lab Overview
Today was the first Dry Lab meeting for the new project! We discussed the mechanics and optimization of general filtration systems. We also started looking for the best way to make hollow fiber reactors the focal point of our filtration system.