Difference between revisions of "Team:MSU-Michigan/Experiments"

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                     <button onclick="myFunction('openclose7')" class="w3-btn w3-block w3-black w3-left-align">LB Agar Plates</button>
 
                     <button onclick="myFunction('openclose7')" class="w3-btn w3-block w3-black w3-left-align">LB Agar Plates</button>
 
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                     <div id="openclose7" class="w3-container w3-hide">
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                        <h5>Materials</h5>
 
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                            <li><h5>Materials</h5></li>
 
 
                             <li>LB powder (Various, currently Miller Acumedic)</li>
 
                             <li>LB powder (Various, currently Miller Acumedic)</li>
 
                             <li>Bacto Agar (BD)</li>
 
                             <li>Bacto Agar (BD)</li>
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                         <h5>Media Preperation</h5>
 
                         <h5>Media Preperation</h5>
 
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                             <li>Add dH20 to autoclavable bottle (500mL in 1L bottle)</li>
 
                             <li>Add dH20 to autoclavable bottle (500mL in 1L bottle)</li>
 
                             <li>Add LB powder (12.5g for 500mL)</li>
 
                             <li>Add LB powder (12.5g for 500mL)</li>
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                             <li>Autoclave for 30 minutes on liquid cycle</li>
 
                             <li>Autoclave for 30 minutes on liquid cycle</li>
 
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                         </ol>
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                        <br>
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                        <h5>Plate Preperation</h5>
 
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                            <li><h5>Plate Preperation</h5></li>
 
 
                             <li>Ensure media is cooled to 50-60°C</li>
 
                             <li>Ensure media is cooled to 50-60°C</li>
 
                             <li>Add antibiotics to final concentratation</li>
 
                             <li>Add antibiotics to final concentratation</li>

Revision as of 15:44, 5 August 2017

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project
Inspiration

General

Title

This procedure is to make LB Broth Media.

Materials
  • LB powder (Various, currently Miller Acumedic)
  • Bacto Agar (BD)
  • dH20
  • Antibiotics
  • Petridishes

Media Preperation
  1. Add dH20 to autoclavable bottle (500mL in 1L bottle)
  2. Add LB powder (12.5g for 500mL)
  3. Add agar (7.5 for 500mL)
  4. Mix using magnetic stir bar or shaking
  5. Autoclave for 30 minutes on liquid cycle

Plate Preperation
  1. Ensure media is cooled to 50-60°C
  2. Add antibiotics to final concentratation
  3. Mix using stir bar or shaking
  4. Pour plates in biosafety hood, ~20mL in each
  5. Let cool with lid askew
  6. When cool stack with top down and slide back in petri dish bag
  7. Tape and Label

Title

This procedure is to make LB Broth Media.

Title

This procedure is to make LB Broth Media.

Testing the Strains

Title

This procedure is to test the fluorescence of modified Shewanella oneidensis MR-1.

Title

This procedure is to test the fluorescence of modified Shewanella oneidensis MR-1.

Title

This procedure is to test the current output of modified Shewanella oneidensis MR-1.

Building Measurement Devices

Purpose:
To create a large-scale liquid biosensor that uses a single chamber to conduct current when inoculated with modified strains of Shewanella Oneidensis and induced by IPTG
Materials:
For each bioreactor:
  • 250 mL mason jars
  • Rubber stoppers (2.5 cm tapering to 2 1/8 cm)
  • Titanium wire (~15cm per unit)
  • Carbon felt
  • Glass reference housing
  • Oxidized nickel wire + Small ~3 mm rubber stoppers
  • Large metal needles for sampling
  • 3 mL plastic syringe (to be cut to act as housing for a counter-electrode)
  • Small magnetic stir bars
  • Needles and syringes of various size (sterile)
Chemicals:
  • Carbon paste suspended in Xylene
  • M5 Minimal Media (100 Mm Hepes)
  • KCl, crystal
  • dH2O
  • Bacto Agar
  • Vitamins/Minerals
  • 200 mM Lactate
  • Spectinomycin Antibiotic
  • IPTG Inducer Stock
Bacterial strains:
  • Shewanella Oneidensis Δmtrb_GFP_mtrb (spec resistance)
  • Shewanella Oneidensis Δmtrb_GFP (spec resistance)
  • Shewanella Oneidensis Δmtrb
Other equipment:
  • Hot/stir plate
  • Multiple stir plate
  • Potentiostat
  • Autoclave

Creating Paper Microbial Fuel Cells

This procedure is to test the current output of modified Shewanella oneidensis MR-1 in a paper cell.