Difference between revisions of "Team:XJTLU-CHINA/InterLab"
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<h1 style="color:white; font-size:80px; padding-top:100px;">Interlab</h1> | <h1 style="color:white; font-size:80px; padding-top:100px;">Interlab</h1> | ||
</div> | </div> | ||
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| + | <div class="container"> | ||
| + | <h1>Background</h1> | ||
| + | <p>The precise and reliable expression of objective genes is a core step in Synthetic Biology. Different combinations of promoters and ribosome binding sites can influence the efficiency of gene expression and even cell growth. Meanwhile, repeatable and comparable measurement is needed in any testing to verify the consistency of results. However, due to different units or different ways of manipulation, it is hard to compare the data between labs.</p> | ||
| + | <hr> | ||
| + | <h1>Aim</h1> | ||
| + | <p>This year’s interlab aims to test some RBS devices that are intended to make gene expression more precise and reliable. Moreover, they establish a GFP measurement protocol to ensure teams to use this same protocol to produce common, comparable units for measuring GFP with different plate readers.</p> | ||
| + | <hr> | ||
| + | <h1>Method</h1> | ||
| + | <h2>1. OD<sub>600</sub> reference</h2> | ||
| + | <h2>2. Fluorescein fluorescence standard curve</h2> | ||
| + | <h2>3. Cell measurement</h2> | ||
| + | <hr> | ||
| + | <h1>Result</h1> | ||
| + | <h2>1. OD<sub>600</sub> reference</h2> | ||
| + | <h2>2. Fluorescein fluorescence standard curve</h2> | ||
| + | <h2>3. Cell measurement</h2> | ||
| + | <hr> | ||
| + | <h1>Discussion</h1> | ||
| + | </div><!-- container --!> | ||
</body> | </body> | ||
Revision as of 03:06, 8 August 2017
Interlab
Background
The precise and reliable expression of objective genes is a core step in Synthetic Biology. Different combinations of promoters and ribosome binding sites can influence the efficiency of gene expression and even cell growth. Meanwhile, repeatable and comparable measurement is needed in any testing to verify the consistency of results. However, due to different units or different ways of manipulation, it is hard to compare the data between labs.
Aim
This year’s interlab aims to test some RBS devices that are intended to make gene expression more precise and reliable. Moreover, they establish a GFP measurement protocol to ensure teams to use this same protocol to produce common, comparable units for measuring GFP with different plate readers.
