Difference between revisions of "Team:XJTLU-CHINA/InterLab"
| Line 27: | Line 27: | ||
<hr> | <hr> | ||
<h1>Method</h1> | <h1>Method</h1> | ||
| − | <h3> | + | <h3>OD<sub>600</sub> reference</h3> |
| − | + | <p>1. Add 100μl of LUDOX into wells A1, B1, C1, D1.</br> | |
| − | <h3> | + | 2. Add 100μl of H<sub>2</sub> O into wells A2, B2, C2 D2.</br> |
| + | 3. Measure absorbance 600 nm of all samples in a microplate reader and record the data.</br> | ||
| + | 4. Add 1ml of LUDOX into cuvette for the measurement of OD600 in spectrophotometer. | ||
| + | </p> | ||
| + | <h3>Fluorescein fluorescence standard curve</h3> | ||
| + | <h3>Cell measurement</h3> | ||
<hr> | <hr> | ||
<h1>Result</h1> | <h1>Result</h1> | ||
| − | <h2> | + | <h2>OD<sub>600</sub> reference</h2> |
| − | + | <p>The average Abs<sub>600</sub> of LUDOX and H<sub>2</sub>O were calculated and showed in the table. The corrected Abs<sub>600</sub> is obtained by using the Abs<sub>600</sub> of H<sub>2</sub>O to minus the Abs<sub>600</sub> of LUDOX. The reference OD<sub>600</sub> was 1ml LUDOX measured by a reference spectrophotometer at 600nm. The value of OD<sub>600</sub>/Abs<sub>600</sub> is 4.25.</p> | |
| − | <h2> | + | <h2>Fluorescein fluorescence standard curve</h2> |
| + | <h2>Cell measurement</h2> | ||
<hr> | <hr> | ||
<h1>Discussion</h1> | <h1>Discussion</h1> | ||
Revision as of 03:13, 8 August 2017
Interlab
Background
The precise and reliable expression of objective genes is a core step in Synthetic Biology. Different combinations of promoters and ribosome binding sites can influence the efficiency of gene expression and even cell growth. Meanwhile, repeatable and comparable measurement is needed in any testing to verify the consistency of results. However, due to different units or different ways of manipulation, it is hard to compare the data between labs.
Aim
This year’s interlab aims to test some RBS devices that are intended to make gene expression more precise and reliable. Moreover, they establish a GFP measurement protocol to ensure teams to use this same protocol to produce common, comparable units for measuring GFP with different plate readers.
Method
OD600 reference
1. Add 100μl of LUDOX into wells A1, B1, C1, D1. 2. Add 100μl of H2 O into wells A2, B2, C2 D2. 3. Measure absorbance 600 nm of all samples in a microplate reader and record the data. 4. Add 1ml of LUDOX into cuvette for the measurement of OD600 in spectrophotometer.
Fluorescein fluorescence standard curve
Cell measurement
Result
OD600 reference
The average Abs600 of LUDOX and H2O were calculated and showed in the table. The corrected Abs600 is obtained by using the Abs600 of H2O to minus the Abs600 of LUDOX. The reference OD600 was 1ml LUDOX measured by a reference spectrophotometer at 600nm. The value of OD600/Abs600 is 4.25.
