Our project aims to create a complete ammonia removal device by combining nitrification and denitrification capabilities within one single bacteria, Paracoccus denitrificans. Nitrification is the conversion of ammonia (NH₃) into nitrite (NO₂^-). Our bacteria is a facultative anaerobe that natively performs denitrification, which is the conversion of nitrites (NO₃^-) and nitrites (NO₂^-) into inert nitrogen gas (N₂). All of the above chemicals, except for nitrogen gas, are not only dangerous to humans, but they also happen to be the nutrients that fuel the process of water eutrophication. Our plan is to transform several genes responsible for nitrification from a nitrifying bacterium, Nitrosomonas europaea, into P. denitrificans. Next, we will perform full-scale characterization of our device and assess its ability to replace the co-culture of bacteria living in Wastewater Activated Sludge (WAS) at wastewater treatment plants.