Difference between revisions of "Team:Hong Kong-CUHK/Notebook"

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<li style="margin-left: 24.0pt; text-indent: -24.0pt;">Project related to pancreatic cancer diagnosis by Toehold Switch is selected</li>
 
<li style="margin-left: 24.0pt; text-indent: -24.0pt;">Project related to pancreatic cancer diagnosis by Toehold Switch is selected</li>
 
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<p>August 2016</p>
 
<p>August 2016</p>
 
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Revision as of 14:03, 10 August 2017

April 2016
  • Settlement of lab duties and position.
  • Lab safety Training.
  • Started regular meetings every week.
May 2016
  • Recruitment of ideas and prepare preliminary data for selection.
  • Project related to pancreatic cancer diagnosis by Toehold Switch is selected

August 2016

  • Cloning of switches and itscorresponding triggers into pSB1A2 and pSB1 with significant “ON/OFF”ratio in vivo.

 

September 2016

  • Identified problems to design Toehold Switch manually and outline programme functions for semi-automatic toehold switch design.
  • Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout.

 

October 2016

  • Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A.

November 2016

  • Brainstorming and literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch.
  • Constructed programs which embedded with Influenza A sequence database and Vienna RNA package.

December 2016

  • Failed to test the system in cell free system due to insufficient materials and resources.
  • T7-rna-polymerase amplification system is cloning to yield higher “ON/OFF”
  • Requested for sponsorship.
  • Skyped the Pakistan iGEM team to explore the potential of collaboration.
  • Continued fundraising.