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| | {{Hong_Kong-CUHK}} | | {{Hong_Kong-CUHK}} |
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| − | <head> | + | <p><a href="https://static.igem.org/mediawiki/2017/e/e2/Notebook.docx.pdf">Notebook</a></p> |
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| − | <button onclick="myFunction()">Click Me</button>
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| − | April 2016
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Settlement of lab duties and position.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Lab safety Training.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Started regular meetings every week.</li>
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| − | </ul>
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| − | May 2016
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Recruitment of ideas and prepare preliminary data for selection.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Project related to pancreatic cancer diagnosis by Toehold Switch is selected</li>
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| − | </ul>
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| − | <p>August 2016</p>
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| − | <div id="myDIV"> | + | |
| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Cloning of switches and itscorresponding triggers into pSB1A2 and pSB1 with significant “ON/OFF”ratio in vivo.</li>
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| − | </ul>
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| − | </div>
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| − | <p> </p>
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| − | <p>September 2016</p>
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| − | <div id="myDIV">
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Identified problems to design Toehold Switch manually and outline programme functions for semi-automatic toehold switch design.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout.</li>
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| − | </ul>
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| − | </div>
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| − | <p> </p>
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| − | <p>October 2016</p>
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| − | <div id="myDIV">
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A.</li>
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| − | </ul> | + | |
| − | <p>November 2016</p>
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| − | <div id="myDIV">
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Brainstorming and literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Constructed programs which embedded with Influenza A sequence database and Vienna RNA package.</li>
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| − | </ul>
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| − | <p>December 2016</p>
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| − | <div id="myDIV">
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| − | <ul>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Failed to test the system in cell free system due to insufficient materials and resources.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">T7-rna-polymerase amplification system is cloning to yield higher “ON/OFF”</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Requested for sponsorship.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Skyped the Pakistan iGEM team to explore the potential of collaboration.</li>
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| − | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Continued fundraising.</li>
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| − | </ul>
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| − | </div>
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| − | </body>
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| | </html> | | </html> |
