1.Came to Peking University and Beijing University of Chemical Technology for project consultation
2.Determined our project:the degradation and utilization of enteromorpha
3.Determined our PI
4.Started to learn basic molecular experiments
5.Prepared experimental material

1.Team registration were completed
2.The circuits, parts, related reagents and equipment of our basic part were identified
3.Tried to explore the pretreatment conditions
4.Started to learn the balance analysis algorithm and programming language for modeling
5.Planned a briefing session for recruitment of next years'team
6.Set up publicity platforms

1.The yeast strain was identified:W303-1A
2.Came to QIBEBT for plasmid desigening and eukaryotic operation technology
3.Came to Shandong University for project consultation
4.Lab safety training for everyone
5.The recuiting started.Related posters and other propaganda had been prepared

1.Strains were identified:The yeast is BY4741,and the E.Coli is KO11
2.Held the recuiting teach-in
3.Started to make co-culture experiment
4.Got the related plasmids pYC230

1.Transformed and recovered pYC230
2.Activited KO11 and BY4741
3.Inoculated,transformed and cultured E.Coli with msa
4.The concept of co-culture between E.Coli and saccharomyces cerevisiae were prooved

1.The first draft of contract was finished
2.Comleted the budget
3.The wiki homepage was designd
4.Sent the primer
5.Held the recruiting interview

1.Sent the plasmids of basic part
2.Drawn the glucose concentration standard curve
3.Recovered plasmid containing J23106 from E.Coli and cellulase from PYC230
4.Publicity brochures was designed

1.Investigate and survey of Seawin Biotech Group
2.The mini system was tested by PCR
3.The gene of BirA was gotten form E.Coli by PCR

1.The yeast backbone pYC was tested by PCR
2.The inp and msa were tested by PCR
3.The birA was sent and synthesised
4.The inp and mas were connected by Gibson assemby
5.inp-msa-J23106 was enzyme linked and transformed into TOP10
6.Bira and pYC230-TP were connected by Gibson assemby and transformed into TOP10
7.Mini system was testd by PCR and connected with pYC230 by Gibson assemby
8.Prepared for national entrepreneurship projects

1.Our team was accepted
2.pUG6 and pSH65-CRE were transformed

1.4 parts of mini system were tested by PCR
2.pYC230 was tested by PCR

1.INP-msA was transformed and recovered
2.The strain with cellulase was in conservation
3.Path of metabolism of xylose was transformed into yeast
4.Related genes synthesising resveratrol were sent to synthesise
5.BAP and pYD1d were sent to sequencing
6.The co-culture medium was made and inoculated
7.The first circuit of mini system:Pmini-Tcyc1 was successfully connect

1.Designed theprimers of BAP and prepared to synthesise
2.INP-msA were successfully connected and recovered
3.The result of co-cuture was not ideal
4.Related genes of resveratrol were synthesised

1.Applied for visa
2.The transform of BirA failed
3.Successfully connected Pcyc1+Tmini and Pmini+Tcyc1
4.The recovering of INP-msA failed
5.The first version of homepage of wiki uploded

1.Detection of cellulase by SDS-PAGE successed
2.The expression of xylose metabolism circuit successe
3.The iGEM kit was received
4.The first circuit of resveratrol synthesis finished
5.The transformation of mini system into yeast successed

1.As a result of preparation for the final exam that we didn't do any experiments

1.Attended 2017 Synthetic Biology Young Schlar Forum in Shanghai

1.The first circuit of resveratrol synthesis was trasformed successfully
2.Determinated resveratrol standard curve by HPLC 3.Started working with Interlab and transformed related circuits 4.BirA connected successfully by Gibson assembly 5.Started connecting OmpA and CenA