Line 427: | Line 427: | ||
</div> | </div> | ||
</div> | </div> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="some-padding"></div> | ||
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
Line 433: | Line 435: | ||
<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P9-collapse" aria-expanded="false" aria-controls="P9-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P9-collapse" aria-expanded="false" aria-controls="P9-collapse"> | ||
<div> | <div> | ||
− | <div class="col-md-11"> | + | <div class="col-md-11">PCR </div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
Line 442: | Line 444: | ||
<div class="panel-body"> | <div class="panel-body"> | ||
<ol> | <ol> | ||
− | <li> | + | <ol> |
+ | <li>Set up PCR mixture as follow :</li> | ||
<table> | <table> | ||
<tr> | <tr> | ||
Line 476: | Line 479: | ||
<tr> | <tr> | ||
<th>Procedures</th> | <th>Procedures</th> | ||
− | <th>Temperature </th> | + | <th>Temperature (°C)</th> |
<th>Duration</th> | <th>Duration</th> | ||
<th>Cycle</th> | <th>Cycle</th> | ||
</tr> | </tr> | ||
+ | <li>Set up thermocycler for PCR reaction as follow:</li> | ||
+ | <li>Add the DNase-free ddH2O in PCR tube. <br></li> | ||
<tr> | <tr> | ||
− | <td>Initial denaturation | + | <td>Initial denaturation </td> |
+ | <td>98</td> | ||
<td>30 s</td> | <td>30 s</td> | ||
<td>1</td> | <td>1</td> | ||
Line 505: | Line 511: | ||
<tr> | <tr> | ||
<td>Sample keping</td> | <td>Sample keping</td> | ||
+ | <td> 4 </td> | ||
<td> ∞ </td> | <td> ∞ </td> | ||
− | <td> | + | <td> 1</td> |
</tr> | </tr> | ||
</table> | </table> | ||
− | |||
<li>Add the DNase-free ddH2O in PCR tube. <br></li> | <li>Add the DNase-free ddH2O in PCR tube. <br></li> | ||
<li>Add the other Component in the tube. If the added volume is less, pipetting up and down | <li>Add the other Component in the tube. If the added volume is less, pipetting up and down |
Revision as of 16:28, 22 August 2017