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<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P3-collapse" aria-expanded="false" aria-controls="P3-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P3-collapse" aria-expanded="false" aria-controls="P3-collapse"> | ||
<div> | <div> | ||
− | <div class="col-md-11">Charaterization | + | <div class="col-md-11">Charaterization: pH stability</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
Line 210: | Line 210: | ||
</div> | </div> | ||
</div> | </div> | ||
+ | <div class="some-padding"></div> | ||
+ | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="P7"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P7-collapse" aria-expanded="false" aria-controls="P7-collapse"> | ||
+ | <div> | ||
+ | <div class="col-md-11">Miniprep </div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> | ||
+ | </div> | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="P7-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P7"> | ||
+ | <div class="panel-body"> | ||
+ | <ol> | ||
+ | <li>Harvest 3 mL cells at 1OD. <br></li> | ||
+ | <li>Centrifuge at 14,000 rpm for 30 s. </li> | ||
+ | <li>Discard the supernatant and resuspend the pellet with remnant.</li> | ||
+ | <li>Resuspend with 250 μl Resuspension Buffer.</li> | ||
+ | <li>Add 250 μl Lysis Buffer. </li> | ||
+ | <li>Add 350 μl Neutralization Buffer.</li> | ||
+ | <li>Centrifuge at 13,000 rpm for 10 mins.</li> | ||
+ | <li>Transfer supernatent to column.</li> | ||
+ | <li>Centrifuge at 13,000 rpm for 1 min.</li> | ||
+ | <li>Add 700 μl Washing Buffer B.</li> | ||
+ | <li>Centrifuge at 13,000 rpm for 1 min.</li> | ||
+ | <li>Column dring with centrifuge 13.000rpm for 1 min.</li> | ||
+ | <li>Inoculate with 30 μl distilled water. </li> | ||
+ | <li>Centrifuge at 13,000 rpm for 1 min.</li> | ||
+ | </ol> | ||
+ | |||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
<div class="some-padding"></div> | <div class="some-padding"></div> | ||
<div class="some-padding"></div> | <div class="some-padding"></div> |
Revision as of 14:27, 23 August 2017