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<li>Steps on extinguishing the fire</li> | <li>Steps on extinguishing the fire</li> |
Revision as of 11:40, 30 August 2017
Safety
Decoding an SDS
Section 1: Identification
- Identifies chemical(s)
- States use of the chemical along with how not to use it
- Provides manufacturer's contact information
Section 2: Hazard(s) Identification
- Identifies hazards of chemicals and classification of harzard (ex: flammable liquid)
- Contains warming information for hazard(s) and precautionary statement(s)
- Pictograms and signal word
- Composition of mixture
Section 3: Compostion/Information on Ingredients
- Identifies ingredient(s) in product along with impurities and stabilizing additives.
- Substances
- Chemical name, synonyms, and Chemical Abstracts Service number
- Impurities and stabilizing additives
- Mixtures
- Same information required for substances
- Concentration for all ingredients that are health hazards and above concentration limit
- Trade secrets will allow the producer to not identify the chemical and/or concentration
Section 4: First-Aid Measures
- Contains necessary first-aid instructions by relevant routes of exposure (inhalation, skin and eye contact, and ingestion)
- Describes symptoms, delayed and present)
- Recommends special treatment and urgent medical care if needed
Section 5: Fire-Fighting Measures
- Steps on extinguishing the fire
- States hazards created from the chemical fire and special PPE needed
Section 6: Accidental Release Measures
- Provides steps on how to respond to releases (spills and leaks included)
- Includes precautions, emergency procedures, containment, and cleanup
Section 7: Handling and Storage
- Provides steps on how to handle and store the chemicals safely.
- Includes precautions on how to handle carefully, to deal with incompatible chemicals, and to minimize environmental release.
- Contains recommendations on storage along with incompatibilities.
Section 8: Exposure Controls/Personal Protection
- Indicates exposure limits and procedures that can be used to limit exposure
- States OSHA (Occupational Safety and Health Administration) exposure limit or other limit
- Includes additional required controls such as exhaust ventilation usage etc
- Recommends personal protective equipment best suited when interacting with chemical
Section 9: Physical and Chemical Properties
- Identifies the chemical's physical and chemical properties. May include but not limited to:
- Appearance and odor
- pH and solubility
- Melting/freezing point and boiling range
- Flammability and explosive limit
- Vapor pressure and vapor density
Section 10: Stability and Reactivity
- Describes reactivity of target chemical(s) when in contact with other chemicals
- Indicates whether the chemical is stable or unstable under room temperature
- Lists the conditions needed to store the chemical
- States potential hazardous reactions that might occur and conditions that should be avoided (high heat, vibrations, electricity)
Section 11: Toxicological Information
- Informs the routes of exposure
- Describes the symptoms and effects of short and long-term exposure
- Indicates whether chemical is a potential carcinogen and toxicity of chemical
Section 12: Ecological Information (non-mandatory)
- Provides information on environmental impact of chemicals if they were released on accident
- States impacts on wildlife, environment, and/or atmosphere
Section 13: Disposal Considerations (non-mandatory)
- Provides guidance on proper disposal procedures
- Describes the correct disposal containers to use
- States special precautions for disposal in landfills
Section 14: Transport Information (non-mandatory)
- Provides protocol for classifying the chemical when transporting it by land, air, or sea
- May contain envrionmental hazard, UN number/name, and precautions on shipping chemical
Section 15: Regulatory Information (non mandatory)
- Identifies remaining safety and regulations not covered elsewhere on SDS
Section 16: Other Information
- Indicates when SDS was last revised or when SDS was actually created
Team Safety
All team members involved in lab procedures were required to adhere to certain lab guidelines as outlined by our advisors. The lab safety rules include but are not limited to:
- Wash in, wash out
- Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material
- Do not eat or drink when in the lab area
- Always use gloves and splash-proof goggles
- Tie back loose hair
- Disinfect all surfaces with 70% ethanol prior to working
- Disinfect all disposable tips, glassware, tubes by soaking in 10% bleach solution for 20 minutes and then disposing in normal waste
- Dispose of growth plates by disposing into a biohazard container which get autoclaved
- Check all equipment for good working order, no chips, torn cords, cracks. Report any issues to an instructor immediately
- When pipetting, don’t touch tip to side of container
- Don’t lay caps of tubes upside down. Use masking tape to hold to bottom of cabinets
- Clean work area with 70% ethanol after working
- Clean up all glassware and labware before leaving lab
- Place all backpacks and stools to the side of the lab to keep walkways clear
- Always know the correct procedure for disposal of lab materials
Public Safety
We took certain measures to ensure that our project did not pose a threat to public safety. All E. coli strains handled were non-pathogenic, and are unlikely to survive in the wild even if released. All biomaterials were disposed of properly and all relevant lab equipment was autoclaved after use. Team members were not allowed outside of the lab while wearing gloves and refrained from touching doorknobs or any surfaces that would come in contact with the public. We cleaned all lab surfaces after use to ensure that others did not come in contact with our biomaterials.
Environmental Safety
To ensure that our project did not propose a hazard to the environment, we used only transformation strains that were purchased through New England Biolabs or from the CSCG Keio Collection. Our project aim is to further characterize the degradation strength of two degradation tags, DAS and LAA, and as such our cell lines will never leave the lab, they will not pose a threat to the environment because they are non pathogenic and contained in a laboratory environment.