Difference between revisions of "Team:Aix-Marseille/Bacteriophages"
(→Phages-like particules design) |
|||
| Line 15: | Line 15: | ||
In this project, we want to creat a M13-like phage like particle, in order to inject toxin in the bacterium. | In this project, we want to creat a M13-like phage like particle, in order to inject toxin in the bacterium. | ||
| − | |||
[[Team:Aix-Marseille/M13_Design|Read more…]] | [[Team:Aix-Marseille/M13_Design|Read more…]] | ||
| − | |||
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 13:56, 31 August 2017
Engineering bacteriophages
M13 phages
M13 is a filamentous phage that infects E. coli that carry the F-episome. Active infection with M13 does not kill the host cell. The M13 phage particle consists of a single-stranded DNA (ssDNA) genome encased in approximately 2700 copies of a major coat protein protein VIII.[1]
Phages-like particules design
Bacteriophages are capable of expressing their genomes, and generating new copies of themselves. We choose to limit the phage ability to reproduce itself in order to contain it. As it is possible to produce recombinant viruses that express foreign proteins, it is possible to restrain their capacity to reproduce themself. [2]
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome. They have been applied not only as prophylactic and therapeutic vaccines but also as vehicles in drug and gene delivery and, more recently, as tools in nanobiotechnology. [2]
In this project, we want to creat a M13-like phage like particle, in order to inject toxin in the bacterium.
References
- ↑ Smeal, S. W., Schmitt, M. A., Pereira, R. R., Prasad, A. & Fisk, J. D. Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation. Virology 500, 259–274 (2017).
- ↑ 2.0 2.1 Roldão, A., Silva, A. C., Mellado, M. C. M., Alves, P. M. & Carrondo, M. J. T. Viruses and Virus-Like Particles in Biotechnology: Fundamentals and Applications. in Reference Module in Life Sciences (Elsevier, 2017).
