Difference between revisions of "Team:Lambert GA/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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<a  href="https://igem.org/2017_Judging_Form?team=Lambert_GA"class="dropbtn">JUDGING FORM</a>
 
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<center> <h1 id="MainTitle"><b> Safety </b></h1> <img src="https://static.igem.org/mediawiki/2017/2/26/T--LambertGA--purpleline.jpg" style="width:18%; margin:auto;"></center>
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<br>
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<center><h3 style="color: #D49AE6;"> Team Safety </h3></Center>
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<p style="width: 75%; margin: auto; font-size: 16px;"><b> All team members involved in lab procedures were required to adhere to certain lab guidelines as outlined by our advisors. The lab safety rules include but are not limited to:</b></p>
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<ul style="width: 75%; margin: auto; font-size: 16px;">
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<li>Wash in, wash out</li>
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<li>Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material</li>
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<li>Do not eat or drink when in the lab area</li>
 +
<li>Always use gloves and splash-proof goggles </li>
 +
<li>Tie back loose hair</li>
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<li>Disinfect all surfaces with 70% ethanol prior to working</li>
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<li>Disinfect all disposable tips, glassware, tubes by soaking in 10% bleach solution for 20 minutes and then disposing in normal waste</li>
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<li>Dispose of growth plates by disposing into a biohazard container which get autoclaved</li>
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<li>Check all equipment for good working order, no chips, torn cords, cracks. Report any issues to an instructor immediately</li>
 +
<li>When pipetting, don’t touch tip to side of container</li>
 +
<li>Don’t lay caps of tubes upside down. Use masking tape to hold to bottom of cabinets</li>
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<li>Clean work area with 70% ethanol after working</li>
 +
<li>Clean up all glassware and labware before leaving lab</li>
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<li>Place all backpacks and stools to the side of the lab to keep walkways clear</li>
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<li>Always know the correct procedure for disposal of lab materials</li>
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</ol>
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<br>
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<br>
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<br>
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<center><h3 style="color: #D49AE6;">Public Safety</h3>
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<p style="width: 100%; margin: auto; font-size: 16px;">We took certain measures to ensure that our project did not pose a threat to public safety. All <i>E. coli</i> strains handled were non-pathogenic, and are unlikely to survive in the wild even if released. All biomaterials were disposed of properly and all relevant lab equipment was autoclaved after use. Team members were not allowed outside of the lab while wearing gloves and refrained from touching doorknobs or any surfaces that would come in contact with the public. We cleaned all lab surfaces after use to ensure that others did not come in contact with our biomaterials.</p></center>
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<br>
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<br>
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<br>
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<center><h3 style="color: #D49AE6;">Environmental Safety</h3></center>
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<p style="width: 100%; margin: auto; font-size: 16px;">To ensure that our project did not propose a hazard to the environment, we used only transformation strains that were purchased through New England Biolabs or from the CSCG Keio Collection. Our project aim is to further characterize the degradation strength of two degradation tags, DAS and LAA, and as such our cell lines will never leave the lab, they will not pose a threat to the environment because they are non pathogenic and contained in a laboratory environment.</p>
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<center><h2>In The Lab</h2></center>
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<center>
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    <img src="https://static.igem.org/mediawiki/2017/9/9b/T--LambertGA--lab1.jpg" style="width:350px;">
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Revision as of 03:11, 25 June 2017


Safety


Team Safety

All team members involved in lab procedures were required to adhere to certain lab guidelines as outlined by our advisors. The lab safety rules include but are not limited to:

  • Wash in, wash out
  • Protect eyes, mucous membranes, open cuts and wounds from contact with biohazard material
  • Do not eat or drink when in the lab area
  • Always use gloves and splash-proof goggles
  • Tie back loose hair
  • Disinfect all surfaces with 70% ethanol prior to working
  • Disinfect all disposable tips, glassware, tubes by soaking in 10% bleach solution for 20 minutes and then disposing in normal waste
  • Dispose of growth plates by disposing into a biohazard container which get autoclaved
  • Check all equipment for good working order, no chips, torn cords, cracks. Report any issues to an instructor immediately
  • When pipetting, don’t touch tip to side of container
  • Don’t lay caps of tubes upside down. Use masking tape to hold to bottom of cabinets
  • Clean work area with 70% ethanol after working
  • Clean up all glassware and labware before leaving lab
  • Place all backpacks and stools to the side of the lab to keep walkways clear
  • Always know the correct procedure for disposal of lab materials



    • Public Safety

    We took certain measures to ensure that our project did not pose a threat to public safety. All E. coli strains handled were non-pathogenic, and are unlikely to survive in the wild even if released. All biomaterials were disposed of properly and all relevant lab equipment was autoclaved after use. Team members were not allowed outside of the lab while wearing gloves and refrained from touching doorknobs or any surfaces that would come in contact with the public. We cleaned all lab surfaces after use to ensure that others did not come in contact with our biomaterials.




    Environmental Safety

    To ensure that our project did not propose a hazard to the environment, we used only transformation strains that were purchased through New England Biolabs or from the CSCG Keio Collection. Our project aim is to further characterize the degradation strength of two degradation tags, DAS and LAA, and as such our cell lines will never leave the lab, they will not pose a threat to the environment because they are non pathogenic and contained in a laboratory environment.




    In The Lab