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| − | <img src="https://i.gyazo.com/b4232621940c0d1c20c6121dff14d204.png" width=90%"> | + | <h1><br><br><br><i>benth</i> Biofactory<br><br><br></h1> |
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| − | <h1> Using Plant Synthetic Biology to generate therapeutics for treatment of thyroid disease. </h1>
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| − | <p><b>The 2017 Cardiff-Wales project has dual aims.</b></p>
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| − | Primarily we will develop tools for plant synthetic biology using the golden gate phytobricks standard. We will take previously characterized promotor elements that respond to a variety of environmental stimuli and move these into the phytobrick standard. These promotor elements will be used to generate TUs that drive reporter gene expression and will represent useful tools for future iGEM teams. </p>
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| − | Secondly we will use these phytobricks to express the composite TSHantag protein, which is an antagonist for Graves Disease, caused by hyperthyroidism. The TSHantag has not previously been used as a therapeutic agent so we will use the tobacco gene expression system to produce high levels of the protein, which will then be tested in a human <i>in vitro</i> system by collaborators at the Cardiff University Medical School.</p>
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| − | We have expertise using the tobacco expression system so will welcome collaborations with iGEM teams who wish to test the function of their proteins in plants! </p>
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| − | <p><b>This promises to be an exciting year of iGEM research!
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| − | </b></p>
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| − | <img src="https://static.igem.org/mediawiki/2017/f/f3/T-Cardiff_Wales-Gilson_Tobacco.jpg"> | + | <p align="left"> <br><br><br><br>Primarily we will develop tools for plant synthetic biology using the golden gate phytobricks standard. We will take previously characterized promotor elements that respond to a variety of environmental stimuli and move these into the phytobrick standard. These promotor elements will be used to generate TUs that drive reporter gene expression and will represent useful tools for future iGEM teams. |
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| | + | Secondly we will use these phytobricks to express the composite TSHantag protein, which is an antagonist for Graves Disease, caused by hyperthyroidism. The TSHantag has not previously been used as a therapeutic agent so we will use the tobacco gene expression system to produce high levels of the protein, which will then be tested in a human <i>in vitro</i> system by collaborators at the Cardiff University Medical School. |
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| | + | We have expertise using the tobacco expression system so will welcome collaborations with iGEM teams who wish to test the function of their proteins in plants! <br><br><br><br><br></p> |
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| − | | + | <img align="right" src ="http://img.webmd.com/dtmcms/live/webmd/consumer_assets/site_images/article_thumbnails/video/how_does_your_thyroid_work_video/375x321_how_does_your_thyroid_work_video.jpg" width="40% " height="450"> |
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| − | <h5>Before you start: </h5> | + | |
| − | <p> Please read the following pages:</p>
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| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | </ul> | + | |
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| − | <h5> Styling your wiki </h5>
| + | <h2 align="center"> Project Abstract </h2> |
| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h5> Wiki template information </h5> | + | |
| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| | + | Grave’s Disease arises from the overproduction of the thyroid stimulating hormone (TSH). Such overproduction leads to increased thyroxine levels, consequently resulting in hyperthyroidism. Our project will involve the expression of the human thyroid stimulating hormone antagonist (TSHantag) using the Tobacco expression system. As of yet, there have been no examples using the tobacco system for expression of the TSHantag protein. Therefore, we will design and generate unique transcriptional units (TUs) for expression of TSHantag in tobacco. These TUs will be introduced into tobacco using agrobacterium-mediated transformation. We will optimise the amounts of heterologous protein that can be produced in this system before collaborating with researchers at University Hospital of Wales to test the efficacy of the TSHantag in an in vitro system. </p> |
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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| − | <a href="https://2017.igem.org/Special:Upload">
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| − | UPLOAD FILES
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