Difference between revisions of "Team:BOKU-Vienna/Protocol"
| Line 122: | Line 122: | ||
<li><a href="#Methods" class="page-scroll">METHODS</a> | <li><a href="#Methods" class="page-scroll">METHODS</a> | ||
<ul> | <ul> | ||
| − | + | <li class="small"><a href="#competentcoli" class="page-scroll">Preparation of chemically competent <i>E. coli</i></a></li> | |
</ul> | </ul> | ||
<ul> | <ul> | ||
| − | <li class="small"><a href="# | + | <li class="small"><a href="#transformationcoli" class="page-scroll">Transformation of chemically competent <i>E. coli</i></a></li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
| − | <li class="small"><a href="# | + | <li class="small"><a href="#tpreparationyeast" class="page-scroll">Preparation of electro competent <i>S. cerevisiae</i></a></li> |
| + | </ul> | ||
| + | <ul> | ||
| + | <li class="small"><a href="#miniprep" class="page-scroll">Plasmid DNA Extraction </a></li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
| Line 135: | Line 138: | ||
<ul> | <ul> | ||
<li class="small"><a href="#q5pcr" class="page-scroll">Q5 PCR</a></li> | <li class="small"><a href="#q5pcr" class="page-scroll">Q5 PCR</a></li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li class="small"><a href="#onetaq" class="page-scroll">oneTaq colony PCR</a></li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
Revision as of 13:08, 15 September 2017
Protocol
Materials
LB medium
| Name | Amount |
|---|---|
| Peptone | 10 g |
| Yeast Extract | 5 g |
| NaCl | 5 g |
| RO-water | 1000 mL |
LB agar
| Name | Amount |
|---|---|
| Peptone | 10 g |
| Yeast Extract | 5 g |
| NaCl | 5 g |
| RO water | 986 mL |
| Agar | 14 g |
Methods
Under construction...
