Basic Parts

During the summer, several basic parts were constructed.

We create basic part for any of our approach :

• Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
• Multi-Tag (Rfc25) - BBa_K2255003

We design 3 parts for the quorum sensing approach :

• Enoyl-CoA hydratase (Rfc10) - BBa_K2255000
• Acyl-CoA isomerase (Rfc10) - BBa_K2255001
• Thioesterase (Rfc10) - BBa_K2255002

We design a part for the disruption of the biofilm :

• EPS-Depolymerase (Rfc25) - BBa_K2255006

Finally, we design some basic part for the phage-like particle approach :

• Domain 3 of p3 from M13 (Rfc25) - BBa_K2255005
• Signal sequence of p3 from M13 (Rfc25) - BBa_K2255007

The collection part use for the creation of phage-like particle, those part correspond to the domaine 1 and 2 of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium we design a large scale of attachment protein :

• p3_E.coli (Rfc25) - BBa_K2255008
• p3_ N.gonorrhoeae (Rfc25) - BBa_K2255009
• p3_P.aeruginosa (Rfc25) - BBa_K2255010
• p3_R.solanacearum_RSM1 (Rfc25) - BBa_K2255011
• p3_R.solanacearum_RSS1 (Rfc25) - BBa_K2255012
• p3_V.Cholerae_CTXΦ (Rfc25) - BBa_K2255013
• p3_V.Cholerae_fs2 (Rfc25) - BBa_K2255014
• p3_V.Cholerea_VGJΦ (Rfc25) - BBa_K2255015
• p3_X.campestris (Rfc25) - BBa_K2255016
• p3_X.fastidiosa (Rfc25) - BBa_K2255017
• p3_X.fuscans (Rfc25) - BBa_K2255018