During the summer, several basic parts were constructed. Some of them are not construct in [http://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] assembly standard, but in [http://parts.igem.org/Assembly_standard_25 Rfc25], which help to create fusion protein.

We create basic part for any of our approach :

• Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - [http://parts.igem.org/Part:BBa_K2255004 BBa_K2255004]
• Multi-Tag (Rfc25) - [http://parts.igem.org/Part:BBa_K2255003 BBa_K2255003]

We design 3 parts for the quorum sensing approach :

• Enoyl-CoA hydratase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255000 BBa_K2255000]
• Acyl-CoA isomerase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]
• Thioesterase (Rfc10) - [http://parts.igem.org/Part:BBa_K2255002 BBa_K2255002]

We design a part for the disruption of the biofilm :

• EPS-Depolymerase (Rfc25) - [http://parts.igem.org/Part:BBa_K2255006 BBa_K2255006]

Finally, we design some basic part for the phage-like particle approach :

• Domain 3 of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255005 BBa_K2255005]
• Signal sequence of p3 from M13 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255007 BBa_K2255007]

The collection part use for the creation of phage-like particle, those part correspond to the domaine 1 (D1) and 2 (D2) of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium, we design a large scale of attachment protein :

• p3_E.coli (Rfc25) - [http://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
• p3_ N.gonorrhoeae (Rfc25) - [http://parts.igem.org/Part:BBa_K2255009 BBa_K2255009]
• p3_P.aeruginosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255010 BBa_K2255010]
• p3_R.solanacearum_RSM1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255011 BBa_K2255011]
• p3_R.solanacearum_RSS1 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255012 BBa_K2255012]
• p3_V.Cholerae_CTXΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255013 BBa_K2255013]
• p3_V.Cholerae_fs2 (Rfc25) - [http://parts.igem.org/Part:BBa_K2255014 BBa_K2255014]
• p3_V.Cholerea_VGJΦ (Rfc25) - [http://parts.igem.org/Part:BBa_K2255015 BBa_K2255015]
• p3_X.campestris (Rfc25) - [http://parts.igem.org/Part:BBa_K2255016 BBa_K2255016]
• p3_X.fastidiosa (Rfc25) - [http://parts.igem.org/Part:BBa_K2255017 BBa_K2255017]
• p3_X.fuscans (Rfc25) - [http://parts.igem.org/Part:BBa_K2255018 BBa_K2255018]