Difference between revisions of "Team:IISER-Mohali-INDIA/InterLab"

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<br />
 
<br />
 
<div>
 
<div>
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<h3>
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OD600 Reference Point
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</h3>
 
<table>
 
<table>
 
     <thead>
 
     <thead>
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</table>
 
</table>
 
<br />
 
<br />
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+
<h2>
 +
Fluorescence Standard Curves
 +
</h2>
 
<table>
 
<table>
 
     <thead>
 
     <thead>
−
        <tr>
+
<tr>
−
<th>&nbsp;</th>
+
<th>uM Fluorescein</th>
−
            <th>LUDOX-HS40</th>
+
<th>50.00</th>
−
            <th>H2O</th>
+
<th>25</th>
−
        </tr>
+
<th>12.5</th>
−
    </thead>
+
<th>6.25</th>
−
    <tbody>
+
<th>3.125</th>
−
        <tr>
+
<th>1.5625</th>
−
            <td>Replicate 1</td>
+
<th>0.78125</th>
−
            <td>0.0932</td>
+
<th>0.390625</th>
−
            <td>0.0973</td>
+
<th>0.1953125</th>
−
       
+
<th>0.09765625</th>
−
        </tr>
+
<th>0.048828125</th>
−
        <tr>
+
<th>0</th>
−
            <td>Replicate 2</td>
+
</tr>
−
            <td>0.0927</td>
+
</thead>
−
            <td>0.0777</td>
+
<tbody>
−
        </tr>
+
<tr>
−
        <tr>
+
<td>Replicate 1</td>
−
            <td>Replicate 3</td>
+
<td>60105</td>
−
            <td>0.0961</td>
+
<td>43268</td>
−
            <td>0.0796</td>
+
<td>29004</td>
−
        </tr>
+
<td class="info">16652</td>
−
        <tr>
+
<td class="info">12162</td>
−
            <td>Replicate 4</td>
+
<td class="info">8151</td>
−
            <td>0.0937</td>
+
<td class="info">8016</td>
−
            <td>0.0803</td>
+
<td class="info">8072</td>
−
        </tr>
+
<td class="info">8362</td>
−
        <tr>
+
<td class="info">7632</td>
−
            <td>Arith. Mean</td>
+
<td class="info">5075</td>
−
            <td>0.093925</td>
+
<td class="info">8434</td>
−
            <td>0.083725</td>
+
</tr>
−
        </tr>
+
<tr>
−
        <tr>
+
<td class="yaxis">Replicate 2</td>
−
            <td>Corrected Abs600</td>
+
<td class="info">34588</td>
−
            <td>0.0102</td>
+
<td class="info">27070</td>
−
        </tr>
+
<td class="info">18993</td>
−
        <tr>
+
<td class="info">18141</td>
−
            <td>Reference OD600</td>
+
<td class="info">11546</td>
−
            <td>0.0425</td>
+
<td class="info">10531</td>
−
        </tr>
+
<td class="info">8528</td>
−
        <tr>
+
<td class="info">8507</td>
−
            <td>OD600/Abs600</td>
+
<td class="info">7813</td>
−
            <td>4.166666667</td>
+
<td class="info">7638</td>
−
        </tr>
+
<td class="info">5168</td>
−
    </tbody>
+
<td class="info">8517</td>
 +
</tr>
 +
<tr>
 +
<td class="yaxis">Replicate 3</td>
 +
<td class="info">34302</td>
 +
<td class="info">28580</td>
 +
<td class="info">20980</td>
 +
<td class="info">16855</td>
 +
<td class="info">11988</td>
 +
<td class="info">9524</td>
 +
<td class="info">9391</td>
 +
<td class="info">8676</td>
 +
<td class="info">8107</td>
 +
<td class="info">7997</td>
 +
<td class="info">5116</td>
 +
<td class="info">8691</td>
 +
</tr>
 +
<tr>
 +
<td class="yaxis">Replicate 4</td>
 +
<td class="info">34386</td>
 +
<td class="info">27353</td>
 +
<td class="info">21253</td>
 +
<td class="info">16614</td>
 +
<td class="info">11953</td>
 +
<td class="info">10064</td>
 +
<td class="info">8787</td>
 +
<td class="info">8667</td>
 +
<td class="info">7569</td>
 +
<td class="info">7550</td>
 +
<td class="info">5128</td>
 +
<td class="info">8053</td>
 +
</tr>
 +
<tr>
 +
<td class="yaxis">Arith. Mean</td>
 +
<td class="warning">34616.25</td>
 +
<td class="warning">27822.75</td>
 +
<td class="warning">20430.25</td>
 +
<td class="warning">17065.5</td>
 +
<td class="warning">11912.25</td>
 +
<td class="warning">9567.5</td>
 +
<td class="warning">8680.5</td>
 +
<td class="warning">8480.5</td>
 +
<td class="warning">7962.75</td>
 +
<td class="warning">7704.25</td>
 +
<td class="warning">5121.75</td>
 +
<td class="warning">8423.75</td>
 +
</tr>
 +
<tr>
 +
<td class="yaxis">Arith. Std.Dev.</td>
 +
<td class="warning">400.2535655</td>
 +
<td class="warning">725.0707437</td>
 +
<td class="warning">1008.13735</td>
 +
<td class="warning">724.7631797</td>
 +
<td class="warning">260.7123255</td>
 +
<td class="warning">1030.082359</td>
 +
<td class="warning">571.830103</td>
 +
<td class="warning">283.1825089</td>
 +
<td class="warning">345.2886474</td>
 +
<td class="warning">199.2525617</td>
 +
<td class="warning">38.28293789</td>
 +
<td class="warning">269.3688611</td>
 +
</tr>
 +
</tbody>
 
</table>
 
</table>
 
</div>
 
</div>
  
 
</html>
 
</html>

Revision as of 20:11, 27 September 2017



OD600 Reference Point

  LUDOX-HS40 H2O
Replicate 1 0.0932 0.0973
Replicate 2 0.0927 0.0777
Replicate 3 0.0961 0.0796
Replicate 4 0.0937 0.0803
Arith. Mean 0.093925 0.083725
Corrected Abs600 0.0102
Reference OD600 0.0425
OD600/Abs600 4.166666667

Fluorescence Standard Curves

uM Fluorescein 50.00 25 12.5 6.25 3.125 1.5625 0.78125 0.390625 0.1953125 0.09765625 0.048828125 0
Replicate 1 60105 43268 29004 16652 12162 8151 8016 8072 8362 7632 5075 8434
Replicate 2 34588 27070 18993 18141 11546 10531 8528 8507 7813 7638 5168 8517
Replicate 3 34302 28580 20980 16855 11988 9524 9391 8676 8107 7997 5116 8691
Replicate 4 34386 27353 21253 16614 11953 10064 8787 8667 7569 7550 5128 8053
Arith. Mean 34616.25 27822.75 20430.25 17065.5 11912.25 9567.5 8680.5 8480.5 7962.75 7704.25 5121.75 8423.75
Arith. Std.Dev. 400.2535655 725.0707437 1008.13735 724.7631797 260.7123255 1030.082359 571.830103 283.1825089 345.2886474 199.2525617 38.28293789 269.3688611