Rstclair2012 (Talk | contribs) |
Rstclair2012 (Talk | contribs) |
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<h5>Cell Measurement Procedure</h5> | <h5>Cell Measurement Procedure</h5> | ||
− | Day 1: transform Escherichia coli DH5α with these following plasmids: | + | <h6>Day 1:</h6> |
+ | transform Escherichia coli DH5α with these following plasmids: | ||
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● Positive control | ● Positive control | ||
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● Test Device 6: J23117.BCD2.E0040.B0015 | ● Test Device 6: J23117.BCD2.E0040.B0015 | ||
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− | Day 2: Picked 2 colonies from each of plate and inoculate it on 5-10 mL LB medium + Chloramphenicol. | + | <h6>Day 2:</h6> |
+ | Picked 2 colonies from each of plate and inoculate it on 5-10 mL LB medium + Chloramphenicol. | ||
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Grow the cells overnight (16-18 hours) at 37°C and 220 rpm. | Grow the cells overnight (16-18 hours) at 37°C and 220 rpm. | ||
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− | Day 3: Cell growth, sampling, and assay | + | <h6>Day 3:</h6> |
+ | Cell growth, sampling, and assay | ||
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◻ Set instrument to read OD600 (as OD calibration setting) | ◻ Set instrument to read OD600 (as OD calibration setting) |
Revision as of 22:02, 29 September 2017
Florida_Atlantic