Difference between revisions of "Team:Amazonas Brazil"

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             <h2>Building a standard BioBrick vector for bacterial genome editing easy as A, B, C… CRISPR</h2>
 
             <h2>Building a standard BioBrick vector for bacterial genome editing easy as A, B, C… CRISPR</h2>
 
               <p>
 
               <p>
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                 The building block of Synthetic Biology is to turn biological system easier to engineer.
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                 The building block of Synthetic Biology is to turn biological systems easier to engineer. Standardization is a fundamental key to that goal. CRISPR/Cas9 machinery paves the way for precisely edit living cell genomes. Although revolutionary, SynBio community has an obstacle: CRISPR/Cas9 protocol is superficially standardized and requires a considerable amount of wet lab work due to the multi-plasmid system. We aim to build a toolbox for one-step genome engineering based on a standard BioBrick vector. To go further and beyond, we unified human practice with pattern recognition and machine learning in order to overcome boundaries in the way of SynBio advancement. We also bring the concept of computational repository for lab "algorithms", the protocols, developing an open and integrated platform to expand the iGEM experience. Our perspective is to leave a legacy and provide a bacterial genome editing machinery based on BioBrick parts assembly easy to engineer as A-B-C… CRISPR.
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                Biological part standardization is a fundamental key for that goal. CRISPR system highlighted
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                a new scientific revolution.  sgRNA specificity associated to the Cas9 programmable activity pave
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                the way for precisely manipulation the genome sequences.
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                However, the synthetic biology community have major obstacles: CRISPR/Cas9 protocol is not standardized
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                and requires laborious time of wet lab work due to the multi-plasmid system. In 2017,
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                our proposal is to develop a novel solution for this core problem, we aim to build a framework
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                for a simple bacterial genome editing based on one standard BioBrick vector. Our perspective is to
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                provide a bacterial genome editing machinery based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
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               </p>
 
               </p>
 
             </div>
 
             </div>

Revision as of 01:54, 2 October 2017

Wiki_iGEM_Amazonas

CRISPeasy

CRISPeasy:

Building a standard BioBrick vector for bacterial genome editing easy as A, B, C… CRISPR

The building block of Synthetic Biology is to turn biological systems easier to engineer. Standardization is a fundamental key to that goal. CRISPR/Cas9 machinery paves the way for precisely edit living cell genomes. Although revolutionary, SynBio community has an obstacle: CRISPR/Cas9 protocol is superficially standardized and requires a considerable amount of wet lab work due to the multi-plasmid system. We aim to build a toolbox for one-step genome engineering based on a standard BioBrick vector. To go further and beyond, we unified human practice with pattern recognition and machine learning in order to overcome boundaries in the way of SynBio advancement. We also bring the concept of computational repository for lab "algorithms", the protocols, developing an open and integrated platform to expand the iGEM experience. Our perspective is to leave a legacy and provide a bacterial genome editing machinery based on BioBrick parts assembly easy to engineer as A-B-C… CRISPR.