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− | <h3><font size="6">Method</font></h3>
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− | <table width="80%">
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− | <thead>
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− | <tr>
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− | <th colspan="2"><u>We follow exactly the interlab protocol provided by iGEM(link) with the below plate reader setting: </u></th>
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− | </tr>
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− | </thead>
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− | <tr>
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− | <th colspan="2">Microplate used: Corning® clear flat bottom black 96 well plates</th>
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− | </tr>
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− | <tr>
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− | <th colspan="2">Instrument used: BMG LABTECH’s CLARIOstar®</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Instrument Settings for measuring OD600 of LUDOX and Cells:</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Endpoint settings</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>No. of flashes per well</th>
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− | <th>10</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Scan mode</th>
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− | <th>orbital averaging</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Scan diameter [mm]</th>
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− | <th>3</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Optic settings</th>
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− | <tr>
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− |
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− | <tr>
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− | <th>Excitation</th>
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− | <th>600</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">General settings</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Top optic used</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Settling time [s]</th>
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− | <th>0.1</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Reading direction</th>
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− | <th>bidirectional, horizontal left to right, top to bottom</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Target temperature [°C]</th>
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− | <th>25</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Instrument Settings for measuring fluorecence of Fluorescein and GFP:</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Endpoint settings</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>No. of flashes per well</th>
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− | <th>8</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Scan mode</th>
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− | <th>orbital averaging</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Scan diameter [mm]</th>
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− | <th>3</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">Optic settings</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Excitation:</th>
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− | <th>470-15</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Emission</th>
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− | <th>515-20</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Gain</th>
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− | <th>500</th>
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− | </tr>
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− |
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− | <tr>
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− | <th>Focal height [mm]</th>
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− | <th>9</th>
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− | </tr>
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− |
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− | <tr>
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− | <th colspan="2">General settings</th>
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− | </tr>
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− |
| |
− | <tr>
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− | <th colspan="2">Top optic used</th>
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− | </tr>
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− |
| |
− | <tr>
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− | <th>Reading direction</th>
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− | <th>bidirectional, horizontal left to right, top to bottom</th>
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− | </tr>
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− |
| |
− | <tr>
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− | <th>Target temperature [°C]</th>
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− | <th>25
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− | </th>
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− | </tr>
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− |
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− | </table>
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| <h3>Experiment</h3> | | <h3>Experiment</h3> |