Difference between revisions of "Team:KUAS Korea/Description"

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<h4>Agar Utilizing Dualcore EMFC</h4>
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<p><font size=4px>EFCs and MFCs have been studied for many years using variety of biological fuels. Many obstacle of these fuel cells is that they often use food sources for humans. Also they only produce a small amount of electricity. The goal of our project is to improve both of these problems. We used agar as the fuel, which is very abundant in the ocean but is not a primary food source for humans. We also combined traditional EFC and MFC to create EMFC which uses <em>shewanella oneidensis</em> MR-1 and diaphorase simultaneously to generate electricity. First, E.coli that displays agar degrading enzymes degrade agar to produce galactose and NADH. Galactose is then used by E.coli to produce lactate and formate which <em>shewanella oneidensis</em> can use to generate electricity. NADH is used by diaphorase to generate electricity as well.</font> </p>
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                         <img src="https://static.igem.org/mediawiki/2016/d/d2/Korea_U_Seoul_figure1.jpeg">
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                         <img src="https://2017.igem.org/File:Heme_final.pdf">
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<h4>2. Prepare catholyte and anolyte</h4><br>
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<p><font size=4>The hssR and hssS mechanism of our project is as follows.</font></p>
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<p> <font size=4>      ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>
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      ② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.<br>
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      ③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.<br>
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      ④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.<br>
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<h4>2. Prepare catholyte and anolyte</h4><br>
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<p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p>
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<p> <font size=4>      ①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>
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      ② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br>
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      ③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br>
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Revision as of 11:54, 7 October 2017

Description

Gelectricell

2. Prepare catholyte and anolyte


The hssR and hssS mechanism of our project is as follows.

① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.



2. Prepare catholyte and anolyte


Also, the mechanism of heme detection using hrtR protein is as follows.

①If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.